The influence of hydrogel modulus on the proliferation and differentiation of encapsulated neural stem cells

Banerjee, Akhilesh; Arha, Manish; Choudhary, Soumitra; Ashton, Randolph S.; Bhatia, Surita R.; Schaffer, David V.; Kane, Ravi S.

doi:10.1016/j.biomaterials.2009.05.050

Cited by 589 publications

(541 citation statements)

References 41 publications

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“…Thus the elastic modulus of polymer hydrogels can influence their migration, development and differentiation [7][8]. Hydrogels also serve as mimics for the extracellular matrix (ECM), which is known to influence the adhesion, geometry and proliferation of cells within their environment [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…Alginates with high GulA content tend to yield hydrogels with greater mechanical stiffness and strength than those with high ManA content [22]. The elastic modulus depends on the number density of physical crosslinks between chains, conferred by the presence of cations [11]. Alginate hydrogels formed with slower rates of gelation tend to exhibit greater structural homogeneity and therefore larger modulus than those gelled rapidly [23].…”

Section: Introductionmentioning

confidence: 99%

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Mechanical properties of alginate hydrogels manufactured using external gelation

Kaklamani

Cheneler

Grover

et al. 2014

Journal of the Mechanical Behavior of Biomedical Materials

163

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Alginate hydrogels are commonly used in biomedical applications such as scaffolds for tissue engineering, drug delivery, and as a medium for cell immobilization. Multivalent cations are often employed to create physical crosslinks between carboxyl and hydroxyl moieties on neighbouring polysaccharide chains, creating hydrogels with a range of mechanical properties. This work describes the manufacture and characterisation of sodium alginate hydrogels using the divalent cations Mg 2+ , Ca 2+ and Sr 2+ to promote gelation via noncovalent crosslinks. The gelation time and Young's modulus are characterised as a function of cation and alginate concentrations. The implications of this work towards the use of environmental elasticity to control stem cell differentiation are discussed.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mechanical properties of alginate hydrogels manufactured using external gelation

Kaklamani

Cheneler

Grover

et al. 2014

Journal of the Mechanical Behavior of Biomedical Materials

163

View full text Add to dashboard Cite

show abstract

“…A decrease in NSC proliferation in 3-D alginate scaffolds is correlated with an increase in the material modulus, where the greatest differentiation expression was attributed to the softest hydrogels. These soft hydrogels possess an elastic modulus comparable to brain tissue (100-1,000 Pa) (Banerjee et al, 2009). In addition to mechanical constraints, the initiation of the cellular and molecular cascade following transplantation must be considered.…”

Section: Considerations For Development Of An Ideal Scaffoldmentioning

confidence: 99%

Stroke Repair via Biomimicry of the Subventricular Zone

Matta

Gonzalez

2018

Front. Mater.

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Stroke is among the leading causes of death and disability worldwide, 85% of which are ischemic. Current stroke therapies are limited by a narrow effective therapeutic time and fail to effectively complete the recovery of the damaged area. Magnetic resonance imaging of the subventricular zone (SVZ) following infarct/stroke has allowed visualization of new axonal connections and projections being formed, while new immature neurons migrate from the SVZ to the peri-infarct area. Such studies suggest that the SVZ is a primary source of regenerative cells for the repair and regeneration of stroke-damaged neurons and tissue. Therefore, the development of tissue engineered scaffolds that serve as a bioreplicative SVZ niche would support the survival of multiple cell types that reside in the SVZ. Essential to replication of the human SVZ microenvironment is the establishment of microvasculature that regulates both the healthy and stroke-injured blood-brain barrier, which is dysregulated poststroke. In order to reproduce this niche, understanding how cells interact in this environment is critical, in particular neural stem cells, endothelial cells, pericytes, ependymal cells, and microglia. Remodeling and repair of the matrix-rich SVZ niche by endogenous reparative mechanisms may then support functional recovery when enhanced by an artificial niche that supports the survival and proliferation of migrating vascular and neuronal cells. Critical considerations to mimic this area include an understanding of resident cell types, delivery method, and the use of biocompatible materials. Controlling stem cell survival, differentiation, and migration are key factors to consider when transplanting stem cells. Here, we discuss the role of the SVZ architecture and resident cells in the promotion and enhancement of endogenous repair mechanisms. We elucidate the interplay between the extracellular matrix composition and cell interactions prior to and following stroke. Finally, we review current cell and neuronal niche biomimetic materials that allow for a tissue-engineered approach in order to promote structural and functional restoration of neural circuitry. By creating an artificial mimetic SVZ, tissue engineers can strive to facilitate tissue regeneration and functional recovery.

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“…The combination of hydrogel bioprinting with melt extrusion has been successfully explored to design well-defined 3D constructs with improved mechanical properties, which is of special interest for load bearing tissues [80], but of limited application in soft tissues. The printing process of viscous bioinks might affect the distribution of cells throughout the construct ultimately leading to tissue heterogeneity, while highly crosslinked hydrogels may lead to excessively stiff matrices that impose severe restrictions to cell spreading, migration and proliferation [11,24,130]. Thus, it is crucial to ensure that the bioink has the appropriate rheological properties to be printed and, at the same time, is able to maintain the shape upon deposition.…”

Section: Hydrogel Bioinksmentioning

confidence: 99%

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

et al. 2017

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Bioprinting technologies are powerful additive biofabrication techniques to produce cellular constructs for skin tissue engineering owing to their unique ability to precisely pattern living and non-living materials in predefined spatial locations. This unique feature, combined with the computer controlled printing and medical imaging techniques, enable researchers and clinicians to generate patient specific constructs partly replicating the intricate compositional and architectural organization of the skin. Bioprinting has been used to automatically dispense hydrogels with skin cells located in prescribed sites that promote skin formation in vitro and in vivo. Current skin bioprinting approaches mostly rely on the sequential printing of fibroblasts and keratinocytes embedded within a homogeneous hydrogel. Although such approaches have already been translated to pre-clinical scenarios, they still present limitations in terms of fully replicating the cellular and extracellular matrix (ECM) heterogeneity in native skin. The success of bioprinting for skin repair strongly depends on the design of printable bioinks capable of supporting the function of printed cells and stimulating the production of new ECM components. To better mimic the human skin, novel developments in dedicated bioprinting technologies, in the design of bioinks, as well as in the printing of vascularised constructs are necessary. This paper presents an overview regarding the use of bioprinting for skin tissue engineering applications. The operating principles of bioprinting technologies are outlined along with requirements of printed skin constructs. Finally, preclinical results are summarized and future perspectives for the field are highlighted.

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The influence of hydrogel modulus on the proliferation and differentiation of encapsulated neural stem cells

Cited by 589 publications

References 41 publications

Mechanical properties of alginate hydrogels manufactured using external gelation

Mechanical properties of alginate hydrogels manufactured using external gelation

Stroke Repair via Biomimicry of the Subventricular Zone

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

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