The influence of hydrogel functional groups on cell behavior

Abstract

This study provides information on the behavior of macrophages on the surface of the subcutaneously implanted hydrogel strips. Hydrogel containing -OH, -CO-NH-, and (CH3)2N- groups induced a spreading of macrophages on the implants. The materials containing -SO3H groups slightly, and materials containing -COOH groups more intensively, inhibited spreading of the macrophages. The fusion of macrophages into multinucleate cells was inhibited on the surface of materials containing acidic groups (-SO3H, -COOH) and increased on the hydrogels containing 30 mol% of alkaline (CH3)2N- groups in comparison with hydrogels containing -OH or -CO -NH- groups. The differences of the behavior of macrophages on the surface of individual types of hydrogels are probably independent on the adsorption of plasma fibronectin onto the hydrogels. The correlation between the macrophages spreading and fusion and surface charge of the hydrogel implant can be hypothetically explained by electrostatic interaction between macrophages cell membrane and implant.

Macrophage recognition of polymers: effect of carboxylate groups

“…The increased electronegativity of the implant surface decreased the in vivo spreading of macrophages and their subsequent fusion in foreign-body giant multinucleate cells, which corresponds to our previous observations [10,11]. The data presented demonstrate that macrophages are able to recognize very subtle differences in the polymer surface chemical structure.…”

Platelet adhesion onto chargeable functional group gradient surfaces

“…After equilibrium with PBS, the functional groups grafted onto the surfaces can be charged differently: carboxylic acid groups for AAgrafted surfaces and sulfonate groups for NaSSgrafted surfaces are negatively chargeable while amine groups for DMAPAA-grafted surfaces are positively chargeable. [23][24][25][26][27] The PRP was incubated on the functional group gradient PE surfaces for 2 h at 37°C. The platelet adhesion on the different functional group gradient surfaces showed a similar trend, as seen in Figure 8: the platelet adhesion on the surfaces decreased gradually as the surface density of functional groups increased along the sample length.…”

Effect of cationic groups on the adhesivity of peritoneal macrophages to polymeric beads

“…Our previous studies demonstrated a remarkable effect of the functional chemical groups in certain polymeric matrices on the adhesion, spreading and fusion of macrophages on the surface of subcutaneously implanted polymers. Anionic groups were found to have an inhibitory influence on these parameters (-COOH >-SO3H ) while cationic groups stimulate macrophage fusion [3][4][5]. Cationized albumin is more actively internalized by macrophages than untreated albumin and anionic sugars of the macrophage surface were identified as the binding site for cationic molecules [6].…”