The influence of GnRH, oxytocin and vasoactive intestinal peptide on the secretion of β-endorphin and production of cAMP and cGMP by porcine pituitary cells in vitro

Bogacka, Iwona; Siawrys, Gabriela; Okrasa, S; Kamiński, Tadeusz; Przala, J

doi:10.1016/s0378-4320(01)00176-2

Cited by 19 publications

(20 citation statements)

References 32 publications

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“…AP tissues from each animal were enzymatically dispersed and then cultured as described previously (Bogacka et al 2002). The number and viabile cells was determined using a haemocytometer and trypan blue (MP Biomedicals, LLC, Santa Ana, CA) exclusion test.…”

Section: Tissue Isolation and Culture Of Anterior Pituitary Cellsmentioning

confidence: 99%

“…In addition, adult females also exhibit a positive, estradiol-dependent feedback of GnRH release as a unique surge-like peak that evokes ovulation by means of the consequent surge of luteinizing hormone release (Counis et al 2009, Naor andHuhtaniemi 2013). Besides GnRH and gonadal steroids, LH synthesis and release is also controlled by stimulatory and inhibitory influences of other hypothalamic-derived hormones such as: vasopressin, oxytocin (Bogacka et al 2002), galanin, substance P, opioids (Estienne and Barb 2005), ghrelin, orexin (Kirsz et al 2014) and recently discovered kisspeptin (Ezzat et al 2010) also controls LH release. Extensive studies, primarily on ruminants and laboratory rodents, have established that leptin might be also directly or indirectly involved in the regulation of LH secretion acting at both the hypothalamic and anterior pituitary levels (Barb et al 2004).…”

Section: Introductionmentioning

confidence: 99%

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In vitro effect of leptin on anterior pituitary cells LH secretory activity during early pregnancy in pig

Siawrys¹,

Gajewska²

2017

Polish Journal of Veterinary Sciences

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Leptin modulates reproductive activity but its potential influence on LH secretion from anterior pituitary (AP) cells during implantation period in pigs (days 14-16 of pregnancy) remained unexplored. This study focused on determination whether leptin affects basal and GnRH-induced LH secretion and intracellular accumulation and whether leptin receptor (OB-Rb) mRNA is expressed in the AP gland during implantation in pigs. Four individual AP glands were developed into separate primary cultures. 2x10 5 cells/ml were preincubated (72 h) and next, for 3.5 h, experimentally treated with GnRH (100 ng/ml), leptin (10 -11 , 10 -9, 10 -7, 10 -6 M) alone, or given in respective combinations with GnRH. In the AP gland, OB-Rb mRNA expression was determined by real-time PCR method. Leptin activated LH secretion and its concentration-dependent effect was observed as stimulation shown in a full range tested (culture 1) and exhibited only at 10 -6 M (culture 2). A pooled data analysis revealed that basal LH secretion increased at 10 -9 , 10 -7 and 10 -6 M, but GnRH-induced LH release decreased at 10 -6 M. Leptin down-regulated GnRH-induced LH secretion in all cultures, but only culture 3 exhibited sensitivity for all concentrations tested. Basal LH accumulation was activated in culture 1 (at 10 -11 M) and inhibited in culture 4 (at 10 -9 M). In the presence of GnRH leptin up-regulated LH accumulation with individual culture leptin-sensitivity (culture 1-3), while down-regulated LH accumulation in culture 4. Obtained data indicate that OB-Rb mRNA is expressed in the AP gland and leptin alone and in combination with GnRH specifically modulates LH activity during early pregnancy in pigs.

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Section: Tissue Isolation and Culture Of Anterior Pituitary Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro effect of leptin on anterior pituitary cells LH secretory activity during early pregnancy in pig

Siawrys¹,

Gajewska²

2017

Polish Journal of Veterinary Sciences

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“…The observation possibly reflected a relationship, which under physiological conditions may be a part of mechanism responsible for 'extinction' of the preovulatory LH surge. This hypothesis was discussed in our previous report (Bogacka et al, 2002b).…”

Section: The Effect Of Gnrh α-And β-Adrenergic Agents On β-End Secrementioning

confidence: 67%

“…The study of Barb et al (1990) has shown that β-END is capable of decreasing LH release from cultured porcine pituitary cells. On the basis of our data, endogenous catecholamines can be appended to a group of factors -besides GnRH (as considered above), OT, and VIP (Bogacka et al, 2002b) -which, transiently acting in concert (through pituitary β-END), may participate in the LH surge termination in sows.…”

Section: The Effect Of Gnrh α-And β-Adrenergic Agents On β-End Secrementioning

confidence: 98%

“…Many neuropeptides, such as thyrotropin-releasing hormone (TRH), vasopressin, neurotensin, galanin and the recently discovered PRL-releasing peptide (PrRP) could promote PRL release from pituitary (Lamberts and MacLeod, 1990;Ben-Jonathan and Liu, 1992;Hammond et al, 1996;Hinuma et al, 1998). In turn, release of β-END -proopiomelanocortin (POMC) processing product, co-released with ACTH -is mainly enhanced by corticotropin-releasing hormone (CRH), as well as by substance P, cholecystokinine (CCK), vasopressin, oxytocin (OT) and vasoactive intestinal peptide (VIP) (Matsumura et al, 1982(Matsumura et al, , 1983Sweep and Wiegant, 1989;Bogacka et al, 2002a). Catecholamines and gonadotrophinreleasing hormone (GnRH) are other candidates, which may influence pituitary PRL and β-END secretion (Denef and Baes, 1982;Gambacciani et al, 1988;Kerdelhue et al, 1988;Shin and Barton, 1993;Denef, 1994;Colthorpe et al, 2000).…”

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confidence: 99%

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The effects of GnRH and adrenergic agents on PRL and ß-endorphin secretion by porcine pituitary cells in vitro

Siawrys

Bogacka

Okrasa

et al. 2003

Acta Veterinaria Hungarica

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The direct effects of α-and β-adrenergic agents on PRL and β-endorphin (β-END) secretion in vitro by porcine pituitary cells have been investigated. Pituitary glands were obtained from mature gilts, which were ovariectomised (OVX) one month before slaughter. Ovariectomised gilts, assigned to four groups, were primed with: (1) vehicle (OVX); (2) and (3) oestradiol benzoate (EB; 2.5 mg/100 kg b.w.) at 30-36 h (OVX+EB I) and 60-66 h (OVX+EB II) before slaughter, respectively; and (4) progesterone (P 4 ; 120 mg/100 kg b.w.) for 5 consecutive days before slaughter (OVX+P 4 ). Isolated anterior pituitary cells were submitted to 3.5 h incubation in the presence of GnRH, α-and β-adrenergic agonists [phenylephrine (PHEN) and isoproterenol (ISOP), respectively], or α-and β-adrenergic blockers [phentolamine (PHENT) and propranolol (PROP), respectively]. The culture media were assayed for PRL (exp. I) and β-endorphin-like immunoreactivity (β-END-LI) (experiment II). In experiment I, GnRH did not influence PRL release by pituitary cells in all experimental groups. Some of tested doses of adrenergic agonists, PHEN and ISOP, increased PRL release from pituitary cells of OVX gilts, but not from those of OVX+EB I animals. In the OVX+EB II group, PHEN alone, but ISOP with PROP, potentiated PRL secretion by the cells. In OVX+P 4 animals, PHEN alone or in combination with PHENT and also ISOP alone or with PROP enhanced PRL output from the cells. In experiment II, addition of GnRH increased β-END-LI release from pituitary cells only in the OVX+EB II group. PHEN and PHENT potentiated β-END-LI secretion by pituitary cells in OVX+EB II and OVX+P 4 groups, while ISOP and PROP increased β-END-LI secretion by the cells of OVX and OVX+EB II animals. In turn, in the OVX+EB I group, effect of PHENT and PROP on PRL secretion by pituitary cells was inhibitory. In conclusion, our results suggest that adrenergic agents can modulate PRL and β-END secretion by porcine pituitary cells in a manner dependent on the hormonal status of gilts.

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Effect of kisspeptin and RFamide-related peptide-3 on the synthesis and secretion of LH by pituitary cells of pigs during the estrous cycle

Zmijewska

Czelejewska

Dziekoński

et al. 2020

Animal Reproduction Science

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The influence of GnRH, oxytocin and vasoactive intestinal peptide on the secretion of β-endorphin and production of cAMP and cGMP by porcine pituitary cells in vitro

Cited by 19 publications

References 32 publications

In vitro effect of leptin on anterior pituitary cells LH secretory activity during early pregnancy in pig

In vitro effect of leptin on anterior pituitary cells LH secretory activity during early pregnancy in pig

The effects of GnRH and adrenergic agents on PRL and ß-endorphin secretion by porcine pituitary cells in vitro

Effect of kisspeptin and RFamide-related peptide-3 on the synthesis and secretion of LH by pituitary cells of pigs during the estrous cycle

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