The influence of extraction method on the composition and analgesic activity of <i>Calligonum comosum</i> phenolic extracts

Chouikh, Atef; Rebiai, Abdelkrim

doi:10.2478/auoc-2020-0007

Cited by 4 publications

(1 citation statement)

References 17 publications

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“…The amount of total phenolics content in extract was determined according to Folin-Ciocalteu's method of Singleton-Rossi (Chouikh et al, 2020b) with slight modification; 0.2 ml of sample solution were introduced into a test tube containing 1 ml of Folin-Ciocalteu reagent (10%), and 0.8 ml of Na2CO3 (7.5%).…”

Section: Determination Of Total Phenolic Content (Tpc)mentioning

confidence: 99%

Comparative study of some physicochemical and biological properties of effect host species variation on the relationship Saharan parasitic plant Cistanche violaceae (Desf.) Beck.

ALIA¹,

Chouikh²,

Djahra³

et al. 2021

Not Sci Biol

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This work aims to study the effect of different host species on physicochemical and biological properties of the Saharan parasitic plant Cistanche violacea that grows parasitized on two hosts Haloxylon articulatum and a Limonistrum guyonianum in the eastern desert of Algeria. The physicochemical characteristics of C. violcea showed an affinity for the results of both ash and pH and it showed a difference in the amount content of carbohydrates and the value of electrical conductivity. For the content of polyphenols, flavonoids, anthocyanins and tannins the highest values were recorded in C. violcea, which was parasitized on H. articulatum. While, the results of HPLC have identified nine compounds in the crude extracts of the parasitic plants and their hosts in different concentrations. In antioxidant activity, the tannin and anthocyanin extracts from C. violcea parasitized on H. articulatum showed better inhibition of DPPH• radical and best the total antioxidant capacity respectively, but the tannins extract of C. violcea parasitized on L. guyonianum given best reducing power capacity. In SPF assay by UV-Vis spectrophotometry method, all extracts of the parasitic plants showed mild to moderate sun protection. Statically the host variation did not show any significant differences in the physicochemical analysis and the quantitative and qualitative total content of polyphenols. While the significant differences appeared through the antioxidant activity tests, especially between C. violcea and its host H. articulatum, between C. violacea and its host H. articulatum and between the two samples of parasitic plants.

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Section: Determination Of Total Phenolic Content (Tpc)mentioning

confidence: 99%

Comparative study of some physicochemical and biological properties of effect host species variation on the relationship Saharan parasitic plant Cistanche violaceae (Desf.) Beck.

ALIA¹,

Chouikh²,

Djahra³

et al. 2021

Not Sci Biol

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Phytochemical analysis and biological activities of Matthiola livida DC. extracts from Oued-Souf region: Insights into antioxidant and anti-inflammatory potential

Chouikh,

Ben Ali,

Brahim Bousbia

et al. 2024

Acta per tech

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This study aimed to assess the characteristics of Saharan plants by analyzing the chemical composition and evaluating the antioxidant and anti-inflammatory properties of raw extracts from Matthiola livida DC., collected from the Oued-Souf region. The primary metabolites analysis revealed carbo-hydrates as the dominant components, with a concentration of 35.70 ? 0.03 mg/g dry weight. Using the maceration technique, three unrefined extracts (methanolic, ethanolic, and aqueous) were produced, with the aqueous extract yielding the highest output at 14.61%. Total polyphenol content varied from 170.82 to 102.42 mg of GA/g dry extract, and the ethanol extract had the highest flavonoid content at 97.03 ? 4.14 mg of QE/g dry extract. The DPPH? test showed the methanolic extract as the most potent antioxidant, with an IC50 of 153.56 ?g/ml. The ethanolic extract offered the most protection to human erythrocytes, resulting in only 16.93% hemolysis at a concentration of 1 mg/ml. It also showed the strongest anti-inflammatory effects in vitro, with a protein denaturation inhibition equivalent to 11.29?1.10 mg of diclofenac sodium per milligram of dry extract. Additionally, IR spectroscopy identified several functional groups, including hydroxyl (O-H), carbonyl (C=O), and various carbon-hydrogen bonds (C-H, C=C, C-H). Future research should explore the clinical applicability of these findings to enhance the therapeutic potential of Matthiola livida extracts.

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Exploring Therapeutic Potential of Malcolmia Aegyptiaca Spr. and Matthiola Livida DC. Extracts in Rat Models Using Hot-Plate, Writhing and Carrageenan-Induced Paw Edema Tests

Chouikh,

Ben Ali,

Bousbia Brahim

2024

Acta Medica Bulgarica

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Background Malcolmia aegyptiaca (locally known as El Harra) and Matthiola livida (locally known as Chegara) are medicinal plants traditionally used to relieve pain and reduce inflammation. Little is known about the flavonoid content or the analgesie and anti-inflammatory properties of these plants. Aims The aim of the study was to explore the natural therapeutic potential of two xerophytic plants, M. aegyptiaca Spr. and Matthiola livida DC, for analgesic and anti-inflammatory activities using hot-plate, writhing and carrageenan-induced paw edema tests. Materials and Methods Flavonoid content was quantified using the AICI3 as reagent. Analgesic activity was assessed using hot-plate (in concentrations of 30 to 80 mg/kg) and writhing tests (20 and 40 mg/kg) in the rats treated. Anti-inflammatory activity was evaluated using the carrageenan-induced paw edema model in rats treated with two doses (25 and 50 mg/kg) of the two plant extracts. Results M. livida extract had a higher flavonoid concentration compared to M. aegyptiaca extract. Both the M. aegyptiaca and M. livida extracts exhibited dose-dependent analgesic effects in the hot-plate test, with higher doses inducing a stronger and more sustained analgesia. M. aegyptiaca extract displayed weaker dose-dependent anti-nociceptive effects in the writhing test compared to the standard NSAID indomethacin. The anti-nociceptive effects of the M. livida extract were mainly observed at the higher dose in the writhing test. Both extracts demonstrated dose-dependent anti-inflammatory activity in the carrageenan-induced paw edema model, with higher doses exhibiting greater inhibition at later time points. Conclusion The M. aegyptiaca and M. livida methanolic extracts possess analgesic and anti-inflammatory properties, supporting their traditional use for the pain and inflammation management. Further research is needed to elucidate the active components and mechanisms of action responsible for these activities.

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The influence of extraction method on the composition and analgesic activity of Calligonum comosum phenolic extracts

Cited by 4 publications

References 17 publications

Comparative study of some physicochemical and biological properties of effect host species variation on the relationship Saharan parasitic plant Cistanche violaceae (Desf.) Beck.

Comparative study of some physicochemical and biological properties of effect host species variation on the relationship Saharan parasitic plant Cistanche violaceae (Desf.) Beck.

Phytochemical analysis and biological activities of Matthiola livida DC. extracts from Oued-Souf region: Insights into antioxidant and anti-inflammatory potential

Exploring Therapeutic Potential of Malcolmia Aegyptiaca Spr. and Matthiola Livida DC. Extracts in Rat Models Using Hot-Plate, Writhing and Carrageenan-Induced Paw Edema Tests

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