The influence of exercise training status on antigen-stimulated IL-10 production in whole blood culture and numbers of circulating regulatory T cells

Handzlik, Michal K.; Shaw, Andrew J.; Dungey, Maurice; Bishop, Nicolette C.; Gleeson, Michael

doi:10.1007/s00421-013-2614-y

Cited by 73 publications

(71 citation statements)

References 27 publications

(35 reference statements)

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“…However, this is a temporary response and 1 hour after exercise the values returned to pre-exercise values. Because of this, the increase in Tregs observed at Post might simply reflect the increase in total numbers of lymphocytes (Handzlik et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Handzlik et al (2013) showed that high training loads are associated with higher resting Treg cell counts and greater IL-10 production following antigen stimulation. By showing a strong relationship between the increase in the number of Treg and high production of IL-10 in vitro, the authors suggested that athletes engaged in high training loads had a greater ability to secrete IL-10 because of the increase in Treg numbers.…”

Section: Discussionmentioning

confidence: 99%

“…Some researchers showed that exercise increases the number and function of Treg cells in murine models (Wang et al 2012;Uchiyama et al 2015), murine asthma models (Lowder et al 2010) and humans (Lages et al 2008;Handzlik et al 2013;Teixeira et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…An inversion relationship between CD127 and FoxP3 expressed in CD4+CD25++ cells has been described (Liu et al 2006), allowing accurate estimation of this Treg cell population via flow cytometry (Handzlik et al 2013) (Figure 1). Senescent T cells were detected based on the expression of KLRG1 + .…”

Section: Flow Cytometrymentioning

confidence: 99%

“…However, most studies have investigated the acute effects of exercise and there are few research related to chronic effects or longitudinal baseline studies. Evidence suggests that circulating numbers of Tregs increase following an acute bout of strenuous exercise in humans (Handzlik et al 2013). Moreover, evidence of an association between high training load, increased in vitro antigen-stimulated IL-10 production and greater numbers of circulating Tregs, support the hypothesis that Tregs mediate inhibitory effects through increases IL-10 production in highly physically active individuals, and this may play an important role in modifying respiratory infection risk (Gleeson and Walsh 2012;Handzlik et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Lifelong training improves anti-inflammatory environment and maintains the number of regulatory T cells in masters athletes

et al. 2017

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2 AbstractPurpose: The purpose of this study was to quantify and characterize peripheral blood regulatory T cells (Tregs), as well as the IL-10 plasma concentration, in Masters athletes at rest and after an acute exhaustive exercise test. Methods: Eighteen Masters athletes (self-reported training: 24.6±1.83 years; 10.27±0.24 months and 5.45±0.42 hours/week per each month trained) and an age-matched control group of 10 subjects (that never took part in regular physical training) volunteered for this study. All subjects performed an incremental test to exhaustion on a cycle ergometer. Blood samples were obtained before (Pre), 10min into recovery (Post) and 1h after the test (1h). Results: Absolute numbers of Tregs were similar in both groups at rest. Acute exercise induced a significant increase in absolute numbers of Tregs at Post (0.0490.021 to 0.0560.024 x10 9 /L, P=0.029 for Masters; 0.0480.017 to 0.0580.020 x10 9 /L, P=0.037 for control) in both groups. Treg mRNA expression for Foxp3, IL-10 and TGF-β in sorted Tregs was similar throughout the trials in both groups. Masters athletes showed a higher percentage of subjects expressing the FoxP3 (100% for Masters vs. 78% for Controls, P=0.038) and TGF- (89% for Masters vs. 56% for Controls, P=0.002) after exercise and a higher plasma IL-10 concentration (15.3907.032 for Masters vs. 2.4111.117 for control P=0.001, ES =2.57) at all time-points. KLRG1 expression in Tregs was unchanged. Conclusion: Our findings showed that Masters athletes have elevated anti-inflammatory markers and maintain the number of Tregs, may be an adaptive response to lifelong training.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Flow Cytometrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Lifelong training improves anti-inflammatory environment and maintains the number of regulatory T cells in masters athletes

et al. 2017

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Exercise intensity‐dependent immunomodulatory effects on encephalomyelitis

Fainstein

Tyk

Touloumi

et al. 2019

Ann Clin Transl Neurol

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Background Exercise training (ET) has beneficial effects on multiple sclerosis and its animal model experimental autoimmune encephalomyelitis (EAE). However, the intensity‐dependent effects of ET on the systemic immune system in EAE remain undefined. Objective (1) To compare the systemic immune modulatory effects of moderate versus high‐intensity ET protocols in protecting against development of EAE; (2) To investigate whether ET affects autoimmunity selectively, or causes general immunosuppression. Methods Healthy mice performed moderate or high‐intensity treadmill running programs. Proteolipid protein (PLP)‐induced transfer EAE was utilized to examine ET effects specifically on the systemic immune system. Lymph node (LN)‐T cells from trained versus sedentary donor mice were transferred to naïve recipients and EAE severity was assessed, by clinical assessment and histopathological analysis. LN‐T cells derived from donor trained versus sedentary PLP‐immunized mice were analyzed in vitro for proliferation assays by flow cytometry analysis and cytokine and chemokine receptor gene expression using real‐time PCR. T cell‐dependent immune responses of trained versus sedentary mice to the nonautoantigen ovalbumin and susceptibility to Escherichia coli‐induced acute peritonitis were examined. Results High‐intensity training in healthy donor mice induced significantly greater inhibition than moderate‐intensity training on proliferation and generation of encephalitogenic T cells in response to PLP‐immunization, and on EAE severity upon their transfer into recipient mice. High‐intensity training also inhibited LN‐T cell proliferation in response to ovalbumin immunization. E. coli bacterial counts and dissemination were not affected by training. Interpretation High‐intensity training induces superior effects in preventing autoimmunity in EAE, but does not alter immune responses to E. coli infection.

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Physical fitness modulates the expression of CD39 and CD73 on CD4⁺ CD25⁻ and CD4⁺ CD25⁺ T cells following high intensity interval exercise

Dorneles¹,

Silva²,

Peres

et al. 2019

J of Cellular Biochemistry

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Aim: To investigate the impact of physical fitness on the mobilization of CD4 + CD25 − CD39 + and CD4 + CD25 + CD39 + T cells in response to acute exercise.Methods: Fifteen high physical fitness (25.3 ± 1.4 years) and 15 low physical fitness (26.1 ± 1.9 years) men performed a single bout of high-intensity interval exercise (HIIE, 10 bouts of 60 seconds at 85% HRmax intercepted by 75 seconds of recovery at 50% HRmax). Blood lymphocytes were isolated before, immediately after and 1 hour after exercise for assessment of cell surface expression of CD25, CD39, and CD73 on CD4 + T cells. Effector memory T cells (mTeff) were identified by CD4 + CD25 − CD39 + coexpression, and memory regulatory T cells (mTReg) were defined as CD4 + CD25 + CD39 + T cells.Results: Exercise increased CD4 + and CD4 + CD25 + T cell frequencies immediately after followed by a decrease bellow to baseline values at 1 hour after the bout in both low and high physical fitness groups. At baseline, the proportions of mTeff were higher, while mTreg were lower in low physical fitness individuals. The frequency of mTreg increased immediately after HIIE in both groups, and remained higher 1 hour after the bout. However, high physical fitness individuals presented higher mTreg frequency in all periods evaluated. A significantly mobilization of mTeff cells was identified in both groups immediately after HIIE. High physical fitness individuals displayed a decrease in mTeff cells bellow to baseline, while the frequency of mTeff remained higher in low physical fitness group 1 hour after the bout. The peripheral frequency of CD4 + CD25 + CD73 + T cells increased in a similar way immediately after the bout in both groups, returning to the baseline values 1 hour after exercise. No differences in CD4 + CD25 − CD73 + T cells were observed after HIIE in both groups. Conclusion:Our results highlight the impact of physical activity status in the redistribution of CD4 + T cells expressing ectonucleotidases in response to HIIE. K E Y W O R D Saerobic fitness, effector memory T cells, interval exercise, memory regulatory T cells

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The influence of exercise training status on antigen-stimulated IL-10 production in whole blood culture and numbers of circulating regulatory T cells

Cited by 73 publications

References 27 publications

Lifelong training improves anti-inflammatory environment and maintains the number of regulatory T cells in masters athletes

Lifelong training improves anti-inflammatory environment and maintains the number of regulatory T cells in masters athletes

Exercise intensity‐dependent immunomodulatory effects on encephalomyelitis

Physical fitness modulates the expression of CD39 and CD73 on CD4⁺ CD25⁻ and CD4⁺ CD25⁺ T cells following high intensity interval exercise

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The influence of exercise training status on antigen-stimulated IL-10 production in whole blood culture and numbers of circulating regulatory T cells

Cited by 73 publications

References 27 publications

Lifelong training improves anti-inflammatory environment and maintains the number of regulatory T cells in masters athletes

Lifelong training improves anti-inflammatory environment and maintains the number of regulatory T cells in masters athletes

Exercise intensity‐dependent immunomodulatory effects on encephalomyelitis

Physical fitness modulates the expression of CD39 and CD73 on CD4+ CD25− and CD4+ CD25+ T cells following high intensity interval exercise

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Physical fitness modulates the expression of CD39 and CD73 on CD4⁺ CD25⁻ and CD4⁺ CD25⁺ T cells following high intensity interval exercise