The Influence of Ethanolic Root Extracts of <i>Ruellia tuberosa L</i>. on Pancreatic Protease Activity and MDA Level of Rats (<i>Rattus norvegicus</i>) Induced by MLD-STZ

Roosdiana, Anna; Sutrisno, Sutrisno; Mahdi, Chanif; Safitri, Anna

doi:10.1088/1755-1315/217/1/012041

Cited by 4 publications

(5 citation statements)

References 5 publications

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“…A qualitative test has demonstrated that triterpenoid compounds were present in the n-hexane R. tuberosa L. root extracts. Subsequently, an in vivo study of R. tuberosa L. hydroethanolic root extracts was conducted and discovered that the extracts had effects on the decrease of blood glucose and MDA levels and affected serum enzyme activity [11][12][13]. e phytochemical examination carried out in this study found that phytosterol, phenolic, and flavonoid compounds were enclosed in R. tuberosa L. hydroethanolic root extracts [11].…”

Section: Introductionmentioning

confidence: 75%

Histopathological Profiles of Rats (Rattus norvegicus) Induced with Streptozotocin and Treated with Aqueous Root Extracts of Ruellia tuberosa L.

Safitri

Sari

Refsilangi

et al. 2021

Veterinary Medicine International

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Diabetes mellitus (DM) is a serious worldwide health threat since the number of people with DM is forecasted to grow annually. Thus, effective and affordable treatment is urgently needed. Our previous studies used n-hexane and hydroethanolic root extracts of Ruellia tuberosa L. which significantly affected diabetic rats. In this study, aqueous R. tuberosa L. root extracts were used as treatments for the diabetic rat model and their effects were evaluated. Diabetes was generated by the administration of streptozotocin (STZ) at 20 mg/kg within 5 sequential days. Male Wistar rats were orally treated with the extracts and standard drug (metformin 200 mg/kg) and vehicle every day for 4 weeks. Hypoglycemic effects were assessed for normal, diabetic control, standard, and extract-treated groups. Histopathology was also carried out for the pancreatic, hepatic, and kidney tissues. The progression of diabetes was considerably diminished after extract treatment. In treated rats, the highest dose of extract induced a decline in blood glucose and serum malondialdehyde (MDA) levels at 25% and 35%, respectively. Furthermore, aqueous extract of R. tuberosa L. treatment decreased MDA levels in the pancreas by 12%. Histologic examination of the organ tissues of diabetic rats showed deteriorating alterations. After treatment, histopathological damages to the tissues and cells were reversed. The results of the experiments recommend that aqueous extract of R. tuberosa L. has antidiabetic effects on STZ-induced diabetic rats; nevertheless, a higher dose of the aqueous extracts is needed to achieve more significant results.

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Section: Introductionmentioning

confidence: 75%

Histopathological Profiles of Rats (Rattus norvegicus) Induced with Streptozotocin and Treated with Aqueous Root Extracts of Ruellia tuberosa L.

Safitri

Sari

Refsilangi

et al. 2021

Veterinary Medicine International

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“…Preparation of the hydroethanolic extracts was previously reported as in [ 23 ]. Preparations of animal models and all treatments applied were the same as in our previous studies [ 19 ]. In brief, 20 male Wistar rats (body weights 120–180 g) were acquired from the Biosains Institute, Brawijaya University.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, we have conducted an in vivo study on R. tuberosa L . hydroethanolic root extracts and found that the extracts lowered blood glucose levels and MDA levels on serum [ 18 ] and MDA levels on the pancreas [ 19 ] and also had effects on serum enzyme activity [ 20 ]. Phytochemical screening tests conducted on this study discovered that R. tuberosa L. roots extracted with ethanol and water containing mostly phytosterol and flavonoid compounds [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Ruellia tuberosa L. Extract Improves Histopathology and Lowers Malondialdehyde Levels and TNF Alpha Expression in the Kidney of Streptozotocin-Induced Diabetic Rats

Roosdiana

Permata

Fitriani

et al. 2020

Veterinary Medicine International

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Ruellia tuberosa L. is a therapeutic plant that is generally consumed in Indonesian traditional medicine to prevent or cure various illnesses, i.e., diabetes. The current study was conducted to investigate the effects of hydroethanolic root extracts of Ruellia tuberosa L. on the kidney of streptozotocin-induced diabetic Wistar rats. In this study, male Wistar rats were divided into 5 groups: healthy rats (group 1), diabetic rats (group 2), and treated rats which received extract at dosages of 250 (group 3), 375 (group 4), and 500 (group 5) mg/kg body weight for 21 days. Diabetes mellitus was experimentally induced by the administration of five doses of streptozotocin 20 mg/kg body weight within five consecutive days. Significant increases in the value of TNF alpha expression and malondialdehyde (MDA) levels were observed in streptozotocin-induced diabetes rats. Furthermore, severe histological alterations of kidney tissues occurred in the diabetic rats group. After treatment was applied, the value of TNF alpha expression and MDA levels on the kidney decreased considerably p < 0.05 in groups 3, 4, and 5. The optimum dosage was obtained at a dose of 250 mg/kg body weight (group 3), which had 42.24% and 52.70% decrease in TNF alpha expression and MDA levels, respectively. The histopathological profiles of the kidney also showed significant improvements in treated groups. The most prominent recoveries were also shown in group 3. The treatments induced repairment in the glomerular and renal tubular damages in the kidney tissues. To conclude, these results emphasize potentially health valuable properties of hydroethanolic root extracts of R. tuberosa L. in rats with streptozotocin-induced diabetes.

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“…Based on our previous studies, R. tuberosa L. mostly contained flavonoids and phytosterol compounds [15,16]. In addition, the R. tuberosa L. hydroethanolic extracts have showed anti-diabetic activity in an in-vivo study [17][18][19]. The LC-MS analysis revealed that flavonoids compounds, such as cirsimarin, cirsimaritin, and sorbifolin, were shown contained in the extracts [20].…”

Section: Introductionmentioning

confidence: 96%

Molecular Docking Approach of Potential Alpha-glucosidase Inhibitors from Extracts Compounds of R. tuberosa L

Safitri

Sari

Roosdiana³

et al. 2020

JSMARTech

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The present study investigates anti-diabetic capacity of compounds enclosed in the Ruellia tuberosa L. root extracts by molecular simulation approach to examine the potential of those compounds acting as alpha glucosidase inhibitors. Compounds chosen were cirsimarin, cirsimaritin, and sorbifolin; quercetin was used for the reference. Those compounds were downloaded from PubChem database, and human alpha glucosidase 3D structure was obtained from Protein Data Bank. The protein was docked to the flavonoid compounds using HEX 8.0 software and visualized using Discovery Studio 4.1. The interactions of cirsimarin, cirsimaritin, sorbifolin, and quercetin on alpha-glucosidase showed similar binding patterns. They interacted with the active sites of the enzyme, causing inhibition on enzyme activity. The interactions between proteins and ligands were mostly through formation of hydrogen bonds and Van der Waals forces. The binding energy of cirsimarin cirsimaritin, sorbifolin, and quercetin to alpha glucosidase were comparable at -323.3, -279.4, -256.8, and -241.5 kJ/mol, respectively. These confirm that compounds contained in the extracts of R. tuberosa L have capacity to be used as inhibitor for alpha glucosidase.

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The Influence of Ethanolic Root Extracts of Ruellia tuberosa L. on Pancreatic Protease Activity and MDA Level of Rats (Rattus norvegicus) Induced by MLD-STZ

Cited by 4 publications

References 5 publications

Histopathological Profiles of Rats (Rattus norvegicus) Induced with Streptozotocin and Treated with Aqueous Root Extracts of Ruellia tuberosa L.

Histopathological Profiles of Rats (Rattus norvegicus) Induced with Streptozotocin and Treated with Aqueous Root Extracts of Ruellia tuberosa L.

Ruellia tuberosa L. Extract Improves Histopathology and Lowers Malondialdehyde Levels and TNF Alpha Expression in the Kidney of Streptozotocin-Induced Diabetic Rats

Molecular Docking Approach of Potential Alpha-glucosidase Inhibitors from Extracts Compounds of R. tuberosa L

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