The Influence of Disinfectants on Microbial Biofilms of Dairy Equipment

Abstract

The aim of the research was to study the features of formation of dairy equipment microflora, the ability of microorganisms to form biofilms on the noncorrosive steel surface with the different roughness and to determine the effectiveness of disinfectants. It was established, that bacteria of Bacillus, Lactobacillus genera of Enterobacteriaceae family are most often extracted from the dairy equipment after the sanitary processing by modern disinfectors, in less number of cases – staphylococci, enterococci, streptococci and pseudomonades. Extracted bacteria form mainly biofilms of the high and middle density. In 100 % of cases biofilms of the high density were formed by Bacillus spp. and Enterococcus faecalis bacteria. It indicates the fact that at the disinfection of the dairy equipment, only stable bacteria that have the ability to produce a biofilm of the high density, remain on its surface. It was established, that the dairy equipment surface relief, namely roughness, has an influence on the process of biofilm formation in Escherichia coli. On the noncorrosive steel surface with the roughness 0,16±0,065 mcm Escherichia coli form biofilms of the lower density comparing with the surface with the density 0,63–0,072 mcm during 24 hours at the temperature 17 °С. It was established, that working solutions of disinfectants P3-ansep CIP, Eco chlor, Medicarine and Maxidez were more effective as to plankton bacteria. Microorganisms, formed in biofilms, turned out stable to these disinfectants. Most effective disinfectant for the influence on bacteria on biofilms is Р3-oxonia active – 150. So, the obtained data indicate that for the effective sanitary processing of the dairy equipment it is necessary to use disinfectants that influence bacteria in biofilms.

Formation of biofilms on dairy equipment and the influence of disinfectants on them

“…The equipment used in the experiment, as well as the techniques for determining the microbiological indicators of microflora of the equipment, are described in detail in paper [43].…”

Determination of biofilm formation and associated gene detection in Staphylococcus genus isolated from the oral cavity under inflammatory periodontal diseases

“…The results of our previous study [15] showed that during periodontitis, dysbacteriosis characterized by a growing number of opportunistic microorganisms was formed in the oral cavity. In particular, dysbiotic changes were observed in all 180 periodontitis patients investigated.…”

Modeling the process of microbial biofilm formation on stainless steel with a different surface roughness

“…Earlier research has found that dairy equipment and finished products at dairy enterprises release bacteria of the genera Bacillus, Lactobacillus, Staphylococcus, Enterococcus, Pseudomonas, and the family Enterobacteriaceae [2]. Staphylococci refer to the optional-anaerobic fixed bacteria of coccoid forms the size of 0.5×1.5 μm.…”

“…The presence of bacteria at surfaces of technological equipment in the dairy industry is regarded to be an important factor that could lead to the contamination of dairy products; it is considered an important hygienic problem [1]. At the same time, micro-organisms survive at the surfaces of technological equipment owing to the capability to create biofilm forms [2,3]. In this regard, certain hygienic requirements are put to the surface of technological equipment that is used in the dairy industry, especially regarding the mark of steel, relief, and roughness.…”