The influence of different fluoride salts on fluoride-mediated inhibition of peroxidase activity in human saliva

Abbeele, Astrid Vanden; Courtois, Philippe; Pourtois, Michel

doi:10.1016/0003-9969(95)00039-r

Cited by 6 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast to the antioxidant properties of Listerine ® , the stannous fluoride active ingredient of Meridol ® shows a dissimilar influence in vitro, i.e., total inhibition of the peroxidase activity in human saliva (Vanden Abbeele et al 1995), and thus may disturb the clearance of the toxic hydrogen peroxide (H 2 O 2 ) and its derivatives from oral fluids (Hannuksela et al 1994). The enzyme peroxidase is the main agent responsible for decomposition of hydrogen peroxide in saliva (Kraus et al 1957).…”

Section: Discussionmentioning

confidence: 99%

Effect of local antimicrobial agents on excisional palatal wound healing: a clinical and histomorphometric study in rats

Artzi

Hirshberg

Israeli-Tobias

et al. 2007

J Clinic Periodontology

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of local antimicrobial agents on excisional palatal wound healing: a clinical and histomorphometric study in rats

Artzi

Hirshberg

Israeli-Tobias

et al. 2007

J Clinic Periodontology

View full text Add to dashboard Cite

show abstract

“…Here, we describe for the first time a Mn 2+ -independent oxidation of organic substrates catalyzed by heme-containing MnPs exclusively in the presence of fluoride. Fluoride is known to be an inactivator or inhibitor for numerous enzymes − . In heme-containing peroxidases, fluoride binds to the heme iron as a distal ligand and may cause conformational changes in the protein structure by forming hydrogen bonds to amino acid side chains .…”

mentioning

confidence: 99%

Fluoride-Dependent Conversion of Organic Compounds Mediated by Manganese Peroxidases in the Absence of Mn²⁺ Ions

Spiteller

Ullrich

et al. 2010

Biochemistry

View full text Add to dashboard Cite

Manganese peroxidase generally mediates the oxidation of Mn(2+) to Mn(3+) with H(2)O(2) as an oxidant. Several manganese peroxidases purified from different lignin-degrading fungi were found to mediate a fluoride-dependent conversion of organic substrates such as monochlorodimedone or 2,6-dimethoxyphenol in the absence of manganese ions. Using the manganese peroxidase MnP-1 from Bjerkandera adusta strain Ud1, these fluoride-dependent reactions were studied with respect to different substrates converted, reaction products, and kinetic properties to shed some light on the reaction mechanism of manganese peroxidase. The analysis of the reaction products formed from monochlorodimedone and 2,6-dimethoxyphenol showed that the substrates were oxidized rather than fluorinated. The addition of fluoride to MnP-1 resulted in altered absorption spectra, indicating a coordinative binding of fluoride or HF to the heme iron; the fluoride:heme stoichiometry was determined to be 1:1 and the K(D) value to be approximately 2.5 mM at pH 3.4. The high K(D) value indicates weak binding of fluoride to the heme. Fluoride appeared to act as a partially competitive inhibitor with respect to hydrogen peroxide for binding to the heme as the sixth ligand. From the findings, a putative model for the fluoride-dependent reaction was developed. The data were interpreted to indicate that changes of the reaction center of manganese peroxidase as, for example, caused by fluoride binding may lead to the oxidation of organic compounds in the absence of manganese by opening a long-range electron transfer pathway.

show abstract

“…Application of a toothpaste containing sodium monofluorophosphate (41), caused no observable alteration in salivary peroxidase activity. However, it has been reported (20,21) that various fluoride salts can inhibit peroxidase activity. In our investigation, no differences were noted in saliva from adolescents with dental fluorosis, with the exception of the group with fluorosis scores of 4.…”

Section: Discussionmentioning

confidence: 99%

“…The effect of fluoride on the peroxidase activity of whole saliva (20,21) has been shown to be a dose dependent inhibition of the enzyme. It has also been reported that the fluoride remaining after rinsing with a fluoride containing product is able to inhibit the activity of peroxidase (22).…”

Section: Intoroductionmentioning

confidence: 99%

A study of some parameters in stimulated saliva from adolescents with dental fluorosis.

Martins-Gomes

Nicolau

Souza

et al. 2001

Journal of Oral Science

View full text Add to dashboard Cite

In this study, parameters such as the flow rate, buffer capacity, sialic acid, protein and electrolyte concentrations, and amylase and peroxidase activities were analyzed in stimulated whole saliva from adolescents with dental fluorosis. From 135 adolescents (13 and 14 years-old) attending a primary and secondary school in the coastal city of Vitoria-Brazil, 72 were selected to participate in this study. The degree of fluorosis was graded using the TSIF, and was carried out by a calibrated and trained dentist. No variation in the flow rate, pH and buffer capacity, protein concentration or amylase activity was observed between the groups with dental fluorosis and the control group (fluorosis score 0). The peroxidase activity and sialic acid concentration showed some differences compared to the control. Sialic acid concentrations were reduced in the groups with dental fluorosis scores above 2. The concentration of Na was lower in adolescents with dental fluorosis, while Mg concentrations were higher in two fluorosis groups, Ca concentration was reduced in two groups with fluorosis. We conclude, that 13 and 14 year-old adolescents attending a school in the coastal city of Vitoria-Brazil showed no variations relative to some parameters and some variations in relation to others of the salivary parameters studied.

show abstract

The influence of different fluoride salts on fluoride-mediated inhibition of peroxidase activity in human saliva

Cited by 6 publications

References 15 publications

Effect of local antimicrobial agents on excisional palatal wound healing: a clinical and histomorphometric study in rats

Effect of local antimicrobial agents on excisional palatal wound healing: a clinical and histomorphometric study in rats

Fluoride-Dependent Conversion of Organic Compounds Mediated by Manganese Peroxidases in the Absence of Mn²⁺ Ions

A study of some parameters in stimulated saliva from adolescents with dental fluorosis.

Contact Info

Product

Resources

About

The influence of different fluoride salts on fluoride-mediated inhibition of peroxidase activity in human saliva

Cited by 6 publications

References 15 publications

Effect of local antimicrobial agents on excisional palatal wound healing: a clinical and histomorphometric study in rats

Effect of local antimicrobial agents on excisional palatal wound healing: a clinical and histomorphometric study in rats

Fluoride-Dependent Conversion of Organic Compounds Mediated by Manganese Peroxidases in the Absence of Mn2+ Ions

A study of some parameters in stimulated saliva from adolescents with dental fluorosis.

Contact Info

Product

Resources

About

Fluoride-Dependent Conversion of Organic Compounds Mediated by Manganese Peroxidases in the Absence of Mn²⁺ Ions