The influence of different carbon sources on growth and single cell oil production in oleaginous yeasts Apiotrichum brassicae and Pichia kudriavzevii

Burgstaller, Lukas; Löffler, Sebastian; Marcellis, Luca De; Ghassemi, Khatereh; Neureiter, Markus

doi:10.1016/j.nbt.2022.02.003

Cited by 17 publications

(3 citation statements)

References 47 publications

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“…Among the tested carbon sources, only lactose appears to be the least suitable for supporting the growth of both the examined strains, which have probably used the yeast extract present in the medium (YP) to maintain their viability. This possibility was also described by Burgstaller et al ( 2022 ) that reported as the growth of some oleaginous yeast was observed in the respective lactose-containing medium without any decrease in lactose levels as determined by the analytic method. From these observations, we can hypothesize that Z. obscura LS31012019, as well as A. pullulans ATCC 15233, did not possess the enzymatic apparatus able to biochemically elaborate lactose for further processing pathways.…”

Section: Discussionsupporting

confidence: 70%

Growth ability, carbon source utilization and biochemical features of the new specie Zalaria obscura

Campana

Palma

Sisti

2022

World J Microbiol Biotechnol

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This research investigated the characteristics of Zalaria obscura LS31012019 in terms of growth ability in different media (SDB, YPD and TSB) and temperatures (22, 25 and 37 °C), utilization of several carbon sources (Glucose, Fructose, Lactose, Sucrose, Xylose, Glycerol and Mannitol at 5, 2 and 1%) and several biochemical features (total protein content, Glutathione, pigments), in comparison with those of the phylogenetically related Aureobasidium pullulans ATCC 15233. The best growth of Z. obscura LS31012019 was obtained in YPD at 25 °C with the highest OD value (0.45) after 144 h of incubation, similar to that of A. pullulans ATCC 15233 (0.48). Glucose resulted the preferred carbon source for both the considered yeasts but also sucrose resulted in efficacy supporting the growth of Z. obscura LS31012019 and A. pullulans ATCC 15233, for their ability in converting sucrose to glucose and fructose and the latter into glucose. Interestingly, Z. obscura LS31012019 utilized also glycerol and mannitol. The biochemical analysis showed the similarity of protein profile in Z. obscura LS31012019 and A. pullulans ATCC 15233 (from 90 to 20 kDa) and a reduced GSH content (0.321 and 0.233 µmol/mg). The pigments extraction with hexane generated a yellow oleaginous pellet in both the strains, while a yellow solid matrix more intensely coloured in A. pullulans ATTC 15233 was visible with the following solvent extractions. Overall, our data showed that Z. obscura LS31012019 can grow in different media and temperatures and utilize carbon sources apart from glucose and sucrose, shifting to a non-fermentative metabolism. These results improve the information regarding the characteristics of Z. obscura, opening a new field of investigation for the possible application of new species of black yeasts in human application.

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Section: Discussionsupporting

confidence: 70%

Growth ability, carbon source utilization and biochemical features of the new specie Zalaria obscura

Campana

Palma

Sisti

2022

World J Microbiol Biotechnol

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“…Acetic acid (C2) accumulates in hydrolysates from diverse biomasses with acetylated sugar backbone chains being considered a main inhibitor in 2nd generation (2G) biorefinery processes [ 4 – 6 ]. Acetic acid can also be used as a carbon (C) source by several yeast species [ 7 , 8 ], is an inhibitory product of yeast metabolism [ 9 ] and is widely used as food preservative [ 10 ]. Formic acid (C1) is another short-chain inhibitory weak acid present in lignocellulosic hydrolysates [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Shared and more specific genetic determinants and pathways underlying yeast tolerance to acetic, butyric, and octanoic acids

Mota,

Matos,

Bahri

et al. 2024

Microb Cell Fact

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Background The improvement of yeast tolerance to acetic, butyric, and octanoic acids is an important step for the implementation of economically and technologically sustainable bioprocesses for the bioconversion of renewable biomass resources and wastes. To guide genome engineering of promising yeast cell factories toward highly robust superior strains, it is instrumental to identify molecular targets and understand the mechanisms underlying tolerance to those monocarboxylic fatty acids. A chemogenomic analysis was performed, complemented with physiological studies, to unveil genetic tolerance determinants in the model yeast and cell factory Saccharomyces cerevisiae exposed to equivalent moderate inhibitory concentrations of acetic, butyric, or octanoic acids. Results Results indicate the existence of multiple shared genetic determinants and pathways underlying tolerance to these short- and medium-chain fatty acids, such as vacuolar acidification, intracellular trafficking, autophagy, and protein synthesis. The number of tolerance genes identified increased with the linear chain length and the datasets for butyric and octanoic acids include the highest number of genes in common suggesting the existence of more similar toxicity and tolerance mechanisms. Results of this analysis, at the systems level, point to a more marked deleterious effect of an equivalent inhibitory concentration of the more lipophilic octanoic acid, followed by butyric acid, on the cell envelope and on cellular membranes function and lipid remodeling. The importance of mitochondrial genome maintenance and functional mitochondria to obtain ATP for energy-dependent detoxification processes also emerged from this chemogenomic analysis, especially for octanoic acid. Conclusions This study provides new biological knowledge of interest to gain further mechanistic insights into toxicity and tolerance to linear-chain monocarboxylic acids of increasing liposolubility and reports the first lists of tolerance genes, at the genome scale, for butyric and octanoic acids. These genes and biological functions are potential targets for synthetic biology approaches applied to promising yeast cell factories, toward more robust superior strains, a highly desirable phenotype to increase the economic viability of bioprocesses based on mixtures of volatiles/medium-chain fatty acids derived from low-cost biodegradable substrates or lignocellulose hydrolysates.

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“…Most biotechnologically important and industrially utilized are specific strains of Yarrowia lipolytica and Rhodotorula toruloides, although other species have gained much attention in recent years [12][13][14] . In comparison, only a few yeast species have been identified for their ability to utilize SCFAs: Y. lipolytica, Cutaneotrichosporon oleaginosum, Cutaneotrichosporon curvatum, Naganishia albida, Trichosporon cutaneum, Saccharomyces cerevisiae, Rhodotorula toruloides, Rhodotorula glutinis, Apiotrichum brassicae and Pichia kudriavzevii 2,12,13,[16][17][18][19][20][21] . In particular, strains such as Y. lipolytica ACA DC 50 109, Y. lipolytica CICC 31,596, C. curvatum NRRL Y-1511, and C. curvatum ATCC 20,509 have a considerable ability to utilize SCFAs and accumulate lipids [22][23][24] .…”

mentioning

confidence: 99%

High-throughput screening of non-conventional yeasts for conversion of organic waste to microbial oils via carboxylate platform

Žganjar,

Ogrizović,

Matul

et al. 2024

Sci Rep

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Converting waste into high-value products promotes sustainability by reducing waste and creating new revenue streams. This study investigates the potential of diverse yeasts for microbial oil production by utilizing short-chain fatty acids (SCFAs) that can be produced from organic waste and focuses on identifying strains with the best SCFA utilisation, tolerance and lipid production. A collection of 1434 yeast strains was cultivated with SCFAs as the sole carbon source. Eleven strains emerged as candidates with promising growth rates and high lipid accumulation. Subsequent fermentation experiments in liquid SCFA-rich media, which focused on optimizing lipid accumulation by adjusting the carbon to nitrogen (C/N) ratio, showed an increase in lipid content at a C/N ratio of 200:1, but with a concurrent reduction in biomass. Two strains were characterized by their superior ability to produce lipids compared to the reference strain Yarrowia lipolytica CECT124: Y. lipolytica EXF-17398 and Pichia manshurica EXF-7849. Characterization of these two strains indicated that they exhibit a biotechnologically relevant balance between maximizing lipid yield and maintaining growth at high SCFA concentrations. These results emphasize the potential of using SCFAs as a sustainable feedstock for oleochemical production, offering a dual benefit of waste valorisation and microbial oil production.

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The influence of different carbon sources on growth and single cell oil production in oleaginous yeasts Apiotrichum brassicae and Pichia kudriavzevii

Cited by 17 publications

References 47 publications

Growth ability, carbon source utilization and biochemical features of the new specie Zalaria obscura

Growth ability, carbon source utilization and biochemical features of the new specie Zalaria obscura

Shared and more specific genetic determinants and pathways underlying yeast tolerance to acetic, butyric, and octanoic acids

High-throughput screening of non-conventional yeasts for conversion of organic waste to microbial oils via carboxylate platform

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