The Influence of Dietary Chicken Egg Lysozyme on the Growth Performance, Blood Health, and Resistance Against Escherichia coli in the Growing Rabbits' Cecum

El-Deep, M. H.; Amber, Khairy; Eid, Yahya; Al-Rashood, Sara T.; Khan, Haseeb A.; Sakr, Mohamed S; Dawood, Mahmoud A.O.

doi:10.3389/fvets.2020.579576

Cited by 23 publications

(19 citation statements)

References 38 publications

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“…In the present study, the creatine level significantly increased in treated groups due to renal failure that could be due to adverse side effects of amoxicillin on rabbits. Contrary to our result, a significant decrease in the creatinine level was revealed in the LZM-treated rabbit groups [ 14 ] ( Table 4 ).…”

Section: Discussioncontrasting

confidence: 99%

“…In the recent biochemical examination, we observed significantly increased concentrations of HD lipoproteins, LD lipoproteins, serum globulin, and calcium levels in antibiotic amoxicillin-treated groups with respect to the untreated control group. Similar to our results, increased concentrations of serum globulin are similar to LZM-fed rabbits [ 14 ]. In the present study, an increased concentration of high density lipoprotein is similar to another work in which Nigella sativa seed was fed to rabbits (16) A contrary to our result is reported by El-Deep et al when they fed LZM to rabbits and found no significant difference.…”

Section: Discussionsupporting

confidence: 92%

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Deleterious Effects of Amoxicillin on Immune System and Haematobiochemical Parameters of a Rabbit

Hussain

Lashari

Farooq

et al. 2022

BioMed Research International

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The present study is aimed at evaluating the haematobiochemical and immune system alterations in rabbit’s exposure to amoxicillin. Thirty-two healthy rabbits were randomly divided into four ( n = 8 ) groups comprising of three experimental groups and one control group. After 7 days of the acclimatization period, the study animals were given different doses of amoxicillin orally (100, 150, and 200 mg/kg body weight) for 21 days. The hematological results revealed that red blood cells, hemoglobin, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration decreased significantly ( P < 0.05 ) whereas white blood cells, neutrophils, and granulocyte exhibited a significantly increasing trend. Serum biochemical analysis showed a significantly increased concentration of HDL, LDL, serum globulin cholesterol, triglyceride, urea, uric acid, creatine, and calcium while plasma fibrinogen, blood sugar, albumin, and total protein were decreased significantly. Furthermore, liver function enzymes such as alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and bilirubin significantly increased. Antioxidant enzymes and oxidative stress parameters such as malondialdehyde concentrations (MDA) increased significantly while catalase, superoxide dismutase, reduced glutathione, and peroxidase reduced significantly in antibiotic amoxicillin-treated groups as compared to the untreated control group ( P < 0.05 ). Microscopic histopathological examination showed negative structural changes in liver, kidney, and heart tissues due to karyorrhexis; a disorganized hepatic cord in the liver; increased Bowman’s space, necrotic renal tubules, and degenerative glomerulus in the kidney; and necrotic cardiac cells and cytoplasmic vacuolization in the heart, in antibiotic amoxicillin-treated rabbit groups as compared to the control group. In conclusion, amoxicillin induced stress and physiological and immunological impairments due to the adverse effects on haematobiochemical parameters and histopathological and tissue protein changes in target animals.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 92%

Deleterious Effects of Amoxicillin on Immune System and Haematobiochemical Parameters of a Rabbit

Hussain

Lashari

Farooq

et al. 2022

BioMed Research International

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“…Probiotics such as yeast Sacharomyces cerevisiae have been evaluated among others as potential feed supplements to improve animal performance, improve feed utilization, enhance feed digestibility, and reduce the number of pathogens [ 5 ]. Supplementation of the rabbit’s diet with lysozyme (LZM) was able to improve the growth performance and hematological and serum biochemical parameters [ 6 ]. In addition, ginger powder and Chinese herbal medicine supplementation have been reported to improve the production performance, serum metabolites, and antioxidant activity of layer hens [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Clostridium butyricum and a Bacteriophage Cocktail on Growth Performance, Serum Biochemistry, Digestive Enzyme Activities, Intestinal Morphology, Immune Responses, and the Intestinal Microbiota in Rabbits

et al. 2021

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The objective of this study was to assess the effects of dietary supplementation with Clostridium butyricum (CB) and a bacteriophage cocktail (BP) on growth performance, serum biochemical parameters, intestinal digestive and oxidase enzymes, intestinal morphology, immune responses, and the cecum microbiota in rabbits. In total, 108 New Zealand rabbits (5 weeks old) were randomly and equally allotted into three dietary treatment groups (four replicates per treatment, n = 36/treatment): (1) the control (CN) group—rabbits fed the basal diet; (2) CB group—rabbits fed the basal diet supplemented with 100 mg/kg diet Clostridium butyricum; and (3) BP group—rabbits fed the basal diet supplemented with 200 mg/kg diet BP cocktail, respectively, for 6 weeks. Compared with the CN diet, dietary CB and BP inclusion increased the average daily gain (ADG) and average daily feed intake (ADFI) and decreased the feed/gain (F/G) ratio of rabbits. Furthermore, CB increased the digestive enzyme activity (α-amylase and trypsin in the ileum); the chymotrypsin activity was also significantly increased in the duodenum and jejunum. Supplementation with CB significantly enhanced antioxidant capacity (SOD and GSH-Px) in the jejunum and ileum and reduced MDA levels. Additionally, rabbits fed CB had significantly elevated villus height (V) and (V/C) ratios but reduced crypt depth (C). Moreover, dietary CB supplementation markedly increased the ileal expression of tight junction proteins (occludin, ZO-1, and claudin-1) and increased secretory immunoglobulin A (sIgA) production. High-throughput sequencing indicated that the microbiota in the rabbit intestine was altered by CB and BP. Venn diagrams and heatmap plots revealed that the gut microbial community composition varied obviously among rabbits fed different diets. Specifically, CB increased the relative abundance of beneficial bacteria to maintain intestinal barrier homeostasis, whereas BP decreased the relative abundance of Gammaproteobacteria, which included a plenty of pathogenic bacteria.

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“…It is common practice to add enzyme preparations to conventional diets to improve feed digestibility, efficiency, and performance in animals [ 7 , 8 ]. Lysozyme extracted from avian egg whites has been applied in rabbit and broiler production as an effective natural growth promoter and antibacterial agent [ 9 , 10 , 11 ], and it plays an important defense role in the innate immune system in most mammals [ 12 ]. Lysozyme is a 1,4-β-N-acetylmuramidase with antimicrobial effects due to its ability to break down the peptidoglycan found in bacterial cell walls, which results in the loss of cellular membrane integrity, causing cell death [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Exogenous Lysozyme Supplementation on Growth Performance, Caecal Fermentation and Microbiota, and Blood Constituents in Growing Rabbits

Hafsa

Mahmoud

Fayed

et al. 2022

Animals

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The effects of exogenous lysozyme supplementation (LYZ) on growth performance, caecal fermentation and microbiota, and blood characteristics were investigated in growing rabbits. A total of 420 growing male V-Line rabbits (30 d old; weighing 528 ± 16 g) were randomly divided into four groups of 105 rabbits each, and monitored for 42 days. Experimental groups included a control group (LYZ0) fed a basal diet without LYZ supplementation, and three treated groups fed the same basal diet supplemented with LYZ at 50, 100, and 150 mg/kg diet, respectively. The results showed a quadratic improvement in the final body weight, daily growth rate, FCR, and digestibility of DM, while the digestibility of OM, CP, EE, NDF, and ADF improved linearly when LYZ supplementation was increased. The dressing percentage increased quadratically when LYZ levels were increased in the rabbit diets. In rabbits fed LYZ diets, L. acidophilus counts increased linearly (p < 0.05) and L. cellobiosus, and Enterococcus sp. counts increased quadratically, whereas E. coli counts decreased. In the LYZ-supplemented groups, the caecal pH value and NH3-N concentration declined quadratically, whereas total VFA, acetic, and butyric acids increased. Total lipids decreased linearly, whilst triglycerides and cholesterol decreased quadratically with LYZ supplementation. Total antioxidant capacity, superoxide dismutase, glutathione S-transferase, and catalase increased quadratically, while malondialdehyde decreased linearly in the LYZ-supplemented groups. In conclusion, exogenous lysozyme administration improved rabbit growth performance and antioxidant status while lowering the blood lipid profile, altering the bacterial population, and regulating caecal fermentation. Therefore, LYZ up to 150 mg/kg can be used as a potential supplement in rabbit feed.

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The Influence of Dietary Chicken Egg Lysozyme on the Growth Performance, Blood Health, and Resistance Against Escherichia coli in the Growing Rabbits' Cecum

Cited by 23 publications

References 38 publications

Deleterious Effects of Amoxicillin on Immune System and Haematobiochemical Parameters of a Rabbit

Deleterious Effects of Amoxicillin on Immune System and Haematobiochemical Parameters of a Rabbit

Effects of Clostridium butyricum and a Bacteriophage Cocktail on Growth Performance, Serum Biochemistry, Digestive Enzyme Activities, Intestinal Morphology, Immune Responses, and the Intestinal Microbiota in Rabbits

The Effect of Exogenous Lysozyme Supplementation on Growth Performance, Caecal Fermentation and Microbiota, and Blood Constituents in Growing Rabbits

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