The influence of dental metal alloys on cell proliferation and fibronectin arrangement in human fibroblast cultures

Grill, Vittorio; Sandrucci, Maria A.; Basa, M; Lenarda, Roberto Di; Dorigo, E; Narducci, Paola; Martelli, Alberto M.; Delbello, Giorgio; Bareggi, Renato

doi:10.1016/s0003-9969(97)00055-1

Cited by 23 publications

(24 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In our previous investigation, we showed that when fibronectin is organized in both fibrils and adhesion plaques, better biocompatibility of a biomaterial is shown because this fibronectin organization is correlated to higher cell proliferation rates. 5 In the present investigation we found that cultures in the presence of the alloys L/3 and L/11 in which there was a prevalent fibronectin arrangement in fibrils and adhesions plaques presented the highest cell proliferation rate expressed as percentage of BrdU-positive cells at the same culture time. On the contrary, cultures together the L/C alloy exhibited very low fibronectin expression.…”

Section: Discussionsupporting

confidence: 50%

Biocompatibility evaluation of dental metal alloysin vitro: Expression of extracellular matrix molecules and its relationship to cell proliferation rates

Grill

Sandrucci

Lenarda

et al. 2000

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

The biocompatibility in vitro of dental biomaterials has been widely studied, with consideration of cell viability and cell proliferation rates. In the present study we evaluated the biocompatibility in vitro of three single-phase dental metal alloys, all provided by the same manufacturer. To this aim, we considered the percentage of proliferating cells revealed by 5-bromodeoxyuridine incorporation in human fibroblast cultures in the presence of these biomaterials, performing a short time test (72 h). These data were correlated with immunocytochemical expression of four molecules of the extracellular matrix, i.e., fibronectin, type I collagen, beta(1)-integrin subunit, and chondroitin sulfate, because the capability of cells to adhere to substrata is widely related to cell proliferation rates. Alloys presenting higher amounts of noble elements were more biocompatible even when they contained significant amount of both Ag and Cu. As regards the expression of the extracellular matrix molecules, the organization level of fibronectin in fibrils was correlated with higher cell proliferation rates, whereas no difference was detected for the expression of the other antigens. On these bases, we assume that expression of fibronectin could be a useful parameter in evaluation of biocompatibility in addition to cell proliferation capability.

show abstract

Section: Discussionsupporting

confidence: 50%

Biocompatibility evaluation of dental metal alloysin vitro: Expression of extracellular matrix molecules and its relationship to cell proliferation rates

Grill

Sandrucci

Lenarda

et al. 2000

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

show abstract

“…Studies have evaluated the adhesion, proliferation, and metabolism of cells such as 3T3, Balb/c, L929, W138, and human fibroblasts and osteoblasts. 1,2 In the present study, the behavior of mice fibroblasts was assessed by modifications of the parameters of Stanford. 12 This is similar to the studies of Wigg et al 14 and Grill et al, 2 who advocated the investigation of proliferation by microscopic analysis of cell growth and division.…”

Section: Discussionmentioning

confidence: 99%

“…[1][2][3] This is because when metal ions are released into tissue sites, the sites may show symptoms similar to allergic reactions, ie, inflammation in the skin or mucous membranes. The reactions can range from simple erythema to necrosis, depending on the toxicity of the primary irritant, concentration, and exposure time.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Silver Solder Employed in Orthodontics

Freitas

Oshima

Menezes

et al. 2009

The Angle Orthodontist

View full text Add to dashboard Cite

Objective: To test the null hypothesis that the silver soldering employed in orthodontics is not cytotoxic for fibroblasts. Materials and Methods: This in vitro study was performed using a culture of mice fibroblasts (lineage NIH/3T3), divided into four groups (n ϭ 10 each): control, negative control (stainless steel archwire), positive control (amalgam disks), and test group (silver soldering). After cell culture in complete Dulbecco modified eagle medium and achievement of confluence in 80%, the suspension was added to the plates of 24 wells containing the specimens and incubated in an oven at 37ЊC for 24 hours. The plates were analyzed on an inverted light microscope, photomicrographs were obtained, and the results were recorded as response rates based on modifications of the parameters of Stanford according to the size of the diffusion halo of the toxic substance and quantity of cell lysis. Results:The results revealed a maximum response rate for the silver soldering group, as well as severe inhibition of cell proliferation and growth, more round cells with mostly darkened and granular aspects, suggesting lysis with cell death. A similar response was seen in the positive control group. Conclusion: The hypothesis is rejected. The silver soldering used in orthodontics represents a highly cytotoxic material for the cells analyzed. (Angle Orthod. 2009;79:939-944.)

show abstract

“…The biocompatibility of the implant itself is determined by both the physical and chemical characteristics of the material and particular features of implant surface, such as the thickness of the oxide layer, microstructure and porosity. The surface of the dental implant material should enhance firm attachment of the implant to junction epithelium, soft connective tissue and bone 38,112 . In summary, a number of markers produced by osteoblasts or by cells of periodontal ligament have been proposed for testing the dental implant biocompatibility, e.g.…”

Section: Resultsmentioning

confidence: 99%

“…It is also found in association with dental basement membrane during tooth formation, polarization and differentiation of odontoblasts 30,35 . Fibronectin is considered to play important roles in the maintenance of normal tissue order and in interface interactions, since it mediates cell-matrix interactions, recognizing different cell types as well as bacteria 36,37 Fibronectin is also organized in focal adhesions which participate in cell locomotion and in cell adhesion [38][39][40][41] .…”

Section: Fibronectin (Fn)mentioning

confidence: 99%