The Influence of Coating Thickness of Polymer on Chiral Discrimination of Cellulose Tris(3,5-Dimethyl Phenylcarbamate) Chiral Stationary Phase

Abstract

The influence of the coating thickness of the polymer on the chiral discrimination of cellulose tris(3,5-dimethyl phenylcarbamate) (CDMPC) chiral stationary phase (CSP) is investigated. CDMPC-CSPs with different thicknesses of polymer were prepared, and their chromatographic performances were investigated. Thinner coat of polymer will increase the interactions between the samples and the support of CSPs and decrease the selectivity of CSPs. Too thick coat of polymer will not increase the performance of CSPs significantly but will decrease the performance of the column. The suited thickness of polymer is very important for the selectivity of the chiral stationary phase.

HPLC with cellulose Tris (3,5‐DimethylPhenylcarbamate) chiral stationary phase: Influence of coating times and coating amount on chiral discrimination

“…The influence of coating amount on retention factor k 1 ′ could be seen in Figure 9. As shown in Figure 12, the effect of coating amount on column efficiency N was the same as that of previous studies, 22 and an excessive amount of CDMPC affected the intraparticle diffusion of the samples, lowering performance of the columns. In terms of separation factor α (shown in Figure 11B), except for B, they hardly changed with the change of coating amount, which indicated that the coating amount had little effect on the separation factor.…”

“…In terms of separation factor α (shown in Figure 11B), except for B, they hardly changed with the change of coating amount, which indicated that the coating amount had little effect on the separation factor. As shown in Figure 12, the effect of coating amount on column efficiency N was the same as that of previous studies, 22 and an excessive amount of CDMPC affected the intraparticle diffusion of the samples, lowering performance of the columns.…”