The influence of clinostat rotation on the fertilized amphibian egg

Tremor, J. W.; Souza, K. A.

doi:10.1007/bf00928162

Cited by 11 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Egg laying and fertilization of amphibians, fish and birds in a µG environment has been found to be similar to that on earth 2–9 . Interesting findings in the field of reproductive biology have been reported sporadically, for example, eggs developing for a certain period after fertilization in space 10 .…”

Section: Introductionmentioning

confidence: 99%

Human sperm motility in a microgravity environment

Ikeuchi

Sasaki

Umemoto

et al. 2005

Reprod Medicine & Biology

View full text Add to dashboard Cite

Background and Aims:We carried out clinostat and parabolic flight experiments to examine the effects of a microgravity (µG) environment on human sperm motility.Methods: Semen samples were obtained manually from 18 healthy men (aged 27.4 ± 5.4 years) who had given their informed consent. In clinostat experiments, samples that were left stationary were used as a stationary control. Samples rotated vertically and horizontally were used as a rotation control and a clinostat rotation, respectively. In parabolic flight experiments using a jet plane, sperm motility was compared for each parameter at µG, 1G and 2G. The state of 1G during the flight was used as a control. Sperm motility was determined using an automatic motility analyzer HT-M2030 in a microgravity environment.Results: All parameters of sperm motility tended to be lower in clinostat rotation compared with rotation control at both low-speed and high-speed, but the differences were not statistically significant. In parabolic flight, sperm motility and parameters of linear movement were decreased (P < 0.05). There was no significant difference between µG and 2G, but sperm motility was significantly decreased at µG than at 1G. Conclusions:

show abstract

Section: Introductionmentioning

confidence: 99%

Human sperm motility in a microgravity environment

Ikeuchi

Sasaki

Umemoto

et al. 2005

Reprod Medicine & Biology

View full text Add to dashboard Cite

show abstract

“…Because of limited access to space flight, many efforts have been made to establish alternative methods for simulating microgravity on Earth, and one of these—the ‘clinostat’—is considered to be a suitable device. The clinostat mimics µG by ‘nullifying the gravitational vector’ through continuous averaging of G forces using three-dimensional (3D) rotation (see Movie S1) [20]. Moreover, this device can subject the specimen to µG conditions immediately, without exposure to the hypergravity and strong vibrations of a space launch.…”

Section: Introductionmentioning

confidence: 99%

Detrimental Effects of Microgravity on Mouse Preimplantation Development In Vitro

Wakayama¹,

Kawahara

et al. 2009

PLoS ONE

View full text Add to dashboard Cite

Sustaining life beyond Earth either on space stations or on other planets will require a clear understanding of how the space environment affects key phases of mammalian reproduction. However, because of the difficulty of doing such experiments in mammals, most studies of reproduction in space have been carried out with other taxa, such as sea urchins, fish, amphibians or birds. Here, we studied the possibility of mammalian fertilization and preimplantation development under microgravity (µG) conditions using a three-dimensional (3D) clinostat, which faithfully simulates 10–3 G using 3D rotation. Fertilization occurred normally in vitro under µG. However, although we obtained 75 healthy offspring from µG-fertilized and -cultured embryos after transfer to recipient females, the birth rate was lower than among the 1G controls. Immunostaining demonstrated that in vitro culture under µG caused slower development and fewer trophectoderm cells than in 1G controls but did not affect polarization of the blastocyst. These results suggest for the first time that fertilization can occur normally under µG environment in a mammal, but normal preimplantation embryo development might require 1G.

show abstract

“…These systems offer at best a few seconds to minutes of microgravity simulation. Clinostatic rotation is another system that is used to model microgravity for both plant and animal cells [5]. Specifically, clinostats were used to show a suppressed response of lymphocytes to concanavalin A (Con A) that correlated with a suppressed response observed during later orbital flight studies [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Suppressed PHA activation of T lymphocytes in simulated microgravity is restored by direct activation of protein kinase C

Cooper

Pellis

1998

Journal of Leukocyte Biology

View full text Add to dashboard Cite

Utilizing clinostatic rotating wall vessel (RWV) bioreactors that simulate aspects of microgravity, we found phytohemagglutinin (PHA) responsiveness to be almost completely diminished. Activation marker expression was significantly reduced in RWV cultures. Furthermore, cytokine secretion profiles suggested that monocytes are not as adversely affected by simulated microgravity as T cells. Reduced cell-cell and cell-substratum interactions may play a role in the loss of PHA responsiveness because placing peripheral blood mononuclear cells (PBMC) within small collagen beads did partially restore PHA responsiveness. However, activation of purified T cells with cross-linked CD2/CD28 and CD3/CD28 antibody pairs was completely suppressed in the RWV, suggesting a defect in signal transduction. Activation of purified T cells with PMA and ionomycin was unaffected by RWV culture. Furthermore, sub-mitogenic doses of PMA alone but not ionomycin alone restored PHA responsiveness of PBMC in RWV culture. Thus our data indicate that during polyclonal activation the signaling pathways upstream of PKC activation are sensitive to simulated microgravity.

show abstract

The influence of clinostat rotation on the fertilized amphibian egg

Cited by 11 publications

References 13 publications

Human sperm motility in a microgravity environment

Human sperm motility in a microgravity environment

Detrimental Effects of Microgravity on Mouse Preimplantation Development In Vitro

Suppressed PHA activation of T lymphocytes in simulated microgravity is restored by direct activation of protein kinase C

Contact Info

Product

Resources

About