Human sperm motility in a microgravity environment

Ikeuchi, Takahito; Sasaki, Shoichi; Umemoto, Yukihiro; Kubota, Yasue; Kubota, Hirokazu; Kaneko, Tomoyoshi; Kohri, Kenjiro

doi:10.1111/j.1447-0578.2005.00092.x

Cited by 18 publications

(12 citation statements)

References 17 publications

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“…Changes in sperm motility were more pronounced under hypergravity than under microgravity, which is consistent with data obtained on human sperm in parabolic flights [ 11 ]. A decrease in speed under 2g conditions was observed after 1 h, and after 3 h, the number of motile cells begins to decrease.…”

Section: Discussionsupporting

confidence: 91%

Sperm Motility of Mice under Simulated Microgravity and Hypergravity

Biryukov

Zhdankina

2020

IJMS

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For deep space exploration, reproductive health must be maintained to preserve the species. However, the mechanisms underlying the effect of changes in gravity on male germ cells remain poorly understood. The aim of this study was to determine the effect of simulated micro- and hypergravity on mouse sperm motility and the mechanisms of this change. For 1, 3 and 6 h, mouse sperm samples isolated from the caudal epididymis were subjected to simulated microgravity using a random position machine and 2g hypergravity using a centrifuge. The experimental samples were compared with static and dynamic controls. The sperm motility and the percentage of motile sperm were determined using microscopy and video analysis, cell respiration was determined by polarography, the protein content was assessed by Western blotting and the mRNA levels were determined using qRT-PCR. The results indicated that hypergravity conditions led to more significant changes than simulated microgravity conditions: after 1 h, the speed of sperm movement decreased, and after 3 h, the number of motile cells began to decrease. Under the microgravity model, the speed of movement did not change, but the motile spermatozoa decreased after 6 h of exposure. These changes are likely associated with a change in the structure of the microtubule cytoskeleton, and changes in the energy supply are an adaptive reaction to changes in sperm motility.

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Section: Discussionsupporting

confidence: 91%

Sperm Motility of Mice under Simulated Microgravity and Hypergravity

Biryukov

Zhdankina

2020

IJMS

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“…In contrast, in mammals, the speed of sperm movement decreased. This result has been shown in experiments with animals that underwent antiorthostatic suspension [ 22 , 38 ] and during exposure of isolated sperm of humans and mice to simulated microgravity conditions [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 53%

Drosophila melanogaster Sperm under Simulated Microgravity and a Hypomagnetic Field: Motility and Cell Respiration

Burtseva

Biryukov

Zhdankina

et al. 2020

IJMS

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The role of the Earth’s gravitational and magnetic fields in the evolution and maintenance of normal processes of various animal species remains unclear. The aim of this work was to determine the effect of simulated microgravity and hypomagnetic conditions for 1, 3, and 6 h on the sperm motility of the fruit fly Drosophila melanogaster. In addition to the usual diet, the groups were administered oral essential phospholipids at a dosage of 500 mg/kg in medium. The speed of the sperm tails was determined by video recording and analysis of the obtained video files, protein content by western blotting, and cell respiration by polarography. The results indicated an increase in the speed of movement of the sperm tails after 6 h in simulated microgravity. The levels of proteins that form the axoneme of the sperm tail did not change, but cellular respiration was altered. A similar effect occurred with the administration of essential phospholipids. These results may be due to a change in the level of phosphorylation of motor proteins. Exposure to hypomagnetic conditions led to a decrease in motility after 6 h against a background of a decrease in the rate of cellular respiration due to complex I of the respiratory chain. This effect was not observed in the flies that received essential phospholipids. However, after 1 h under hypomagnetic conditions, the rate of cellular respiration also increased due to complex I, including that in the sperm of flies receiving essential phospholipids.

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“…At the same time, the addition of landing overloads neutralizes this effect; immediately after “landing”, the speed of spermatozoa movement is the same as in the control. It was previously shown that the effect of overloads leads to a decrease in the speed of spermatozoa movement [ 26 , 27 , 28 ]. This means that the absence of differences in speed in the sµg+oll and ols+sµg+oll groups from the control group cannot be considered as no effect.…”

Section: Discussionmentioning

confidence: 99%

Sperm of Fruit Fly Drosophila melanogaster under Space Flight

Zhdankina¹,

Kotov²

2022

IJMS

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Studies of reproductive function under long-term space flight conditions are of interest in planning the exploration of deep space. Motility, including the use of various inhibitors, cellular respiration, and the content of cytoskeletal proteins were studied, assessing the level of expression of the corresponding genes in spermatozoa of Drosophila melanogaster, which were in space flight conditions for 12 days. The experiment was carried out twice on board the Russian Segment of the International Space Station. Sperm motility speed after space flight, and subsequently 16 h after landing, is reduced relative to the control by 20% (p < 0.05). In comparison with the simulation experiment, we showed that this occurs as a result of the action of overloads and readaptation to the Earth’s gravity. At the same time, cellular respiration, the content of proteins of the respiratory chain, and the expression of their genes do not change. We used kinase inhibitor 6-(dimethylamino)purine (6-DMAP) and phosphatase inhibitors; 6-DMAP restored the reduced the speed of spermatozoa in the flight group to that of the control. These results can be useful in developing a strategy for protecting reproductive health during the development of other bodies in the solar system.

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Human sperm motility in a microgravity environment

Cited by 18 publications

References 17 publications

Sperm Motility of Mice under Simulated Microgravity and Hypergravity

Sperm Motility of Mice under Simulated Microgravity and Hypergravity

Drosophila melanogaster Sperm under Simulated Microgravity and a Hypomagnetic Field: Motility and Cell Respiration

Sperm of Fruit Fly Drosophila melanogaster under Space Flight

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