The influence of cigarette smoking on human sperm quality and DNA fragmentation

Sepaniak, Sandrine; Forges, Thierry; Gérard, Hubert; Foliguet, B; Béné, Marie‐Christine; Monnier‐Barbarino, Patricia

doi:10.1016/j.tox.2006.03.001

Cited by 172 publications

(129 citation statements)

References 51 publications

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“…The present fi ndings agree with those of previous studies (1,3,(25)(26)(27)(28). On the contrary, some researches reported different results (29)(30)(31)(32). They reported that smoking did not affect conventional semen variables.…”

Section: Discussionsupporting

confidence: 92%

Apoptotic markers in semen of infertile men: association with cigarette smoking

El-Melegy

Ali

2011

Int. braz j urol.

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Objectives: (i) To examine the role of apoptosis in the pathogenesis of DNA damage in semen from infertile men. (ii) To assess the effects of smoking on apoptotic markers and seminal parameters among infertile men. (iii) To assess the correlation of apoptosis with conventional semen parameters. Materials and Methods:The study was carried out on 70 men with idiopathic infertility, divided into two groups: thirty infertile non smokers and forty infertile smokers. In addition to 60 fertile men (30 non smokers and 30 smokers) as control group. Each subject provided semen for analysis of parameters, determination of % of DNA fragmentation, s-Fas, caspase-3 activity levels and cotinine levels. Results:The results revealed that infertile men, particularly smokers have significantly lower semen variables and significantly higher levels of apoptotic variables (% of DNA fragmentation, s-Fas and caspase-3 activity) in addition to cotinine. Conclusions:The present findings provide additional evidence supporting the importance of the evaluation of apoptotic markers to test male infertility particularly among smokers.

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Section: Discussionsupporting

confidence: 92%

Apoptotic markers in semen of infertile men: association with cigarette smoking

El-Melegy

Ali

2011

Int. braz j urol.

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“…Increases in sperm chromosomal abnormalities (Robbins et al, 1997;Rubes et al, 1998;Shi et al, 2001), sperm DNA damage as measured by comet (Hoffmann et al, 2005) TUNEL (Sepaniak et al, 2006) and SCSA (Potts et al, 1999) assays have all been reported in human smokers. Also, it was recently shown in mice that MS smoke induced mutations at specific repeat DNA loci in sperm (Yauk et al, 2007).…”

Section: Discussionmentioning

confidence: 97%

Differential sensitivity of male germ cells to mainstream and sidestream tobacco smoke in the mouse

Polyzos

Schmid

Piña-Guzmán

et al. 2009

Toxicology and Applied Pharmacology

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Cigarette smoking in men has been associated with increased chromosomal abnormalities in sperm and with increased risks for spontaneous abortions, birth defects and neonatal death.Little is known, however, about the reproductive consequences of paternal exposure to secondhand smoke. We used a mouse model to investigate the effects of paternal exposure to sidestream (SS) smoke, the main constituent of second-hand smoke, on the genetic integrity and function of sperm, and to determine whether male germ cells were equally sensitive to mainstream (MS) and SS smoke. A series of sperm DNA quality and reproductive endpoints were investigated after exposing male mice for two weeks to MS or SS smoke. Our results indicated that: (i) only SS smoke significantly affected sperm motility; (ii) only MS smoke induced DNA strand breaks in sperm; (iii) both MS and SS smoke increased sperm chromatin structure abnormalities; and (iv) MS smoke affected both fertilization and the rate of early embryonic development, while SS smoke affected fertilization only. These results show that MS and SS smoke have differential effects on the genetic integrity and function of sperm and provide further evidence that male exposure to second-hand smoke, as well as direct cigarette smoke, may diminish a couple's chance for a successful pregnancy and the birth of a healthy baby.

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“…Outra condição que estabelece um declínio na capacidade reprodutiva masculina de maneira seja controversa na literatura atual 16,17 , existem inúmeros trabalhos demonstrando este efeito deletério do cigarro sobre a fertilidade masculina 14,15 . Uma meta-análise demonstra que pacientes fumantes apresentam um decréscimo médio de 10% na motilidade espermática, 13% na concentração espermática e 3% na morfologia espermática 22 .…”

Section: Variáveisunclassified

“…O volume seminal apresenta-se diminuído em pacientes cigarros dia, quando comparados aos pacientes não fumantes 14,23 . O tabagismo pode igualmente causar diminuição da fertilidade por alterar os níveis hormonais séricos de testosterona e estradiol bem como alteração no DNA dos espermatozóides 14,15 . A fertina medida em que ocorre um decréscimo nas taxas de gravidez e alteração nos parâmetros seminais de pacientes tabagistas 23 .…”

Section: Variáveisunclassified

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A análise seminal deve ser requisitada para homens com histórico de fertilidade prévia?

Pasqualotto¹,

Ferreira²,

Fonseca³

et al. 2006

Rev. Bras. Ginecol. Obstet.

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/mL versus 23,1x10 6 /mL; p=0,07), motilidade (45,2 versus 48,1%; p=0,08) e morfologia (6,1 versus 6,4%; p=0,09) entre os grupos de pacientes com infertilidade primária e secundária. Conclusões: a análise seminal deve ser solicitada mesmo em casos de fertilidade masculina prévia. Os médicos não devem presumir que um paciente possui uma análise seminal normal, baseados no fato de este possuir história de estabelecimento de uma gravidez no passado. PALAVRA-CHAVE:Infertilidade masculina; Semen/análise; Fertilidade; Contagem de espermatozóides Abstract Purpose: to determine if the previous fertility history can predict current fertility status of a patient examined for couple's infertility. Methods: retrospective study involving semen analyses from 183 consecutive subfertile patients evaluated from September 2002 to March 2004. We excluded those patients who had undergone radio or chemotherapy, orchiectomy or vasectomy. Mean values of all analyses were used for patients with multiple semen analysis. Patients with more than 20x10 6 sperm/mL, motility higher than 50% and with normal strict sperm morphology higher than 14% were considered normal. Patients were divided into two groups, according to the fertility status: primary infertility (118 patients) and secondary infertility (65 patients). Data were analyzed according to the 2 test and the Student t-test. Results: no differences were detected in the mean age between patients with primary infertility, 37.3 6.3, and secondary infertility, 38.1 5.9; p=0.08. In the group of patients with primary infertility, 51.9% (61 patients) had a normal sperm concentration, 70.3% (83 patients) had normal sperm motility and 26.3% (31 patients) had normal sperm morphology. In the group of patients with secondary infertility, 53.8% (35 patients) had normal sperm concentration, 75.4% (49 patients) had normal sperm motility and 32.3%Rev Bras Ginecol Obstet. 2006; 28(11): 652-7.

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The influence of cigarette smoking on human sperm quality and DNA fragmentation

Cited by 172 publications

References 51 publications

Apoptotic markers in semen of infertile men: association with cigarette smoking

Apoptotic markers in semen of infertile men: association with cigarette smoking

Differential sensitivity of male germ cells to mainstream and sidestream tobacco smoke in the mouse

A análise seminal deve ser requisitada para homens com histórico de fertilidade prévia?

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