The influence of cAMP before or during bovine oocyte maturation on embryonic developmental competence

Zhang, Guixue; Luciano, A.M.; Coenen, Karine; Gandolfi, F.; Sirard, Marc‐André

doi:10.1016/s0093-691x(01)00516-7

Cited by 49 publications

(36 citation statements)

References 25 publications

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“…The use of compounds, which prevent a decrease of [cAMP]i before IVM, can be useful from the time COCs are isolated from the follicles [22,25,26,28]. This is in agreement with previous findings, where the simple maintenance of intracellular cAMP during COC isolation induces a higher developmental rate than COCs isolated in simple medium [25,32].…”

Section: Discussionsupporting

confidence: 90%

Role of Intracellular Cyclic Adenosine 3′,5′-Monophosphate Concentration and Oocyte-Cumulus Cells Communications on the Acquisition of the Developmental Competence During In Vitro Maturation of Bovine Oocyte1

Luciano

Modina

Vassena

et al. 2004

Biology of Reproduction

138

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The present study was designed to address the physiological role played by cAMP on gap junction (GJ) mediated communications between oocyte and cumulus cells during in vitro maturation. Cyclic AMP was stimulated by different collection and maturation media known to induce different rates of nuclear maturation and developmental competence as well as different levels of cumulus expansion. Cumulus-oocyte complexes (COCs) were matured for 0, 3, 7, 12, 18, and 24 h in the absence of stimulation or in the presence of serum and gonadotropins (fetal bovine serum+human menopausal gonadotropins [FCS+hMG]) or 0.01 microg/ml of invasive adenylate cyclase (iAC). For each time point, intracellular cAMP concentration ([cAMP]i) was determined either in the whole COC or oocyte after cumulus cell removal. GJ functional status was analyzed by microinjection of Lucifer yellow fluorescent dye in cumulus-enclosed oocytes and by immunohistochemical localization of connexin 43 (Cx43). In the absence of stimulation, [cAMP]i in COC and oocyte was lower than in other groups, and communications declined after 3 h of culture. In the FCS+hMG group, [cAMP]i increased significantly in COC, with a peak between 3 and 7 h that was temporally correlated with the beginning of the cumulus expansion process, which occurred only in this group and with the termination of the communications. COC matured in the presence of iAC showed a moderate increase of [cAMP]i during all of the maturation times as well as a prolongation of oocyte-cumulus cell communications. The immunohistochemical localization of Cx43 confirmed the delay in connexons protein turnover in iAC-treated COCs. Our results show that cumulus expansion and oocyte developmental competence are induced by different levels of cAMP and that its intracellular concentration may affect cell coupling between oocyte and cumulus cells. We hypothesize that the higher developmental competence of COCs matured in the presence of iAC could be achieved through a moderate increase of intracellular cAMP, which in turn determines a prolongation of communications between the two cell types.

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Section: Discussionsupporting

confidence: 90%

Role of Intracellular Cyclic Adenosine 3′,5′-Monophosphate Concentration and Oocyte-Cumulus Cells Communications on the Acquisition of the Developmental Competence During In Vitro Maturation of Bovine Oocyte1

Luciano

Modina

Vassena

et al. 2004

Biology of Reproduction

138

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“…In parallel, increases in CC cAMP induce expression of EGF-like peptides and subsequent activation of the EGF receptor and MAPK pathway, thereby stimulating cumulus expansion and oocyte maturation [40][41][42]. It is widely reported that cAMP modulators in cattle IVM extended CC-oocyte GJC and increased oocyte developmental competence [13,19,20,32,43,44].…”

Section: Discussionmentioning

confidence: 99%

Regulation of sheep oocyte maturation using cAMP modulators

et al. 2013

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“…This is usually followed by an IVM phase lacking pharmacological AC activators, either in the presence (Zeng et al 2013) or absence of FSH (Sugimura et al 2015). A PDE inhibitor such as cilostamide can be included in the IVM phase, as used in SPOM version 1 (Albuz et al 2010) or omitted as per SPOM version 2 (Zeng et al 2013, Richani et al 2014b, Zeng et al 2014, Li et al 2016, and in the iAC and dbcAMP approaches (Funahashi et al 1997, Luciano et al 1999, Guixue et al 2001. It is noteworthy that in all induced IVM (high cAMP) approaches, oocyte meiotic maturation is induced as a result of the elevated CC cAMP, even in the presence of a PDE inhibitor (Dekel et al 1988, Shu et al 2008) (this does not or is less likely to occur in biphasic IVM).…”

Section: Induced Ivm (High Camp)mentioning

confidence: 99%

Oocyte maturation and quality: role of cyclic nucleotides

Gilchrist¹,

Luciano²,

Richani³

et al. 2016

Reproduction

175

117

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The cyclic nucleotides, cAMP and cGMP, are the key molecules controlling mammalian oocyte meiosis. Their roles in oocyte biology have been at the forefront of oocyte research for decades, and many of the long-standing controversies in relation to the regulation of oocyte meiotic maturation are now resolved. It is now clear that the follicle prevents meiotic resumption through the actions of natriuretic peptides and cGMP -inhibiting the hydrolysis of intra-oocyte cAMP -and that the pre-ovulatory gonadotrophin surge reverses these processes.

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The influence of cAMP before or during bovine oocyte maturation on embryonic developmental competence

Cited by 49 publications

References 25 publications

Role of Intracellular Cyclic Adenosine 3′,5′-Monophosphate Concentration and Oocyte-Cumulus Cells Communications on the Acquisition of the Developmental Competence During In Vitro Maturation of Bovine Oocyte1

Role of Intracellular Cyclic Adenosine 3′,5′-Monophosphate Concentration and Oocyte-Cumulus Cells Communications on the Acquisition of the Developmental Competence During In Vitro Maturation of Bovine Oocyte1

Regulation of sheep oocyte maturation using cAMP modulators

Oocyte maturation and quality: role of cyclic nucleotides

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