Regulation of sheep oocyte maturation using cAMP modulators

Rose, Ryan; Gilchrist, Robert B.; Kelly, Jennifer; Thompson, Jeremy G.; Sutton‐McDowall, Melanie L.

doi:10.1016/j.theriogenology.2012.09.020

Cited by 73 publications

(52 citation statements)

References 47 publications

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“…Our results on the effect of the use of PDE inhibitors on meiotic resumption are largely supportive of previous data in pre-pubertal gilts in which cilostamide or other PDE3 inhibitors, such as milrinone, have been used [17,67,68]. Effects of cilostamide treatments on in vitro developmental potential have been tested in human [20,21,63], bovine [22,64], ovine [65] and mouse [56,62,64,69] with different outcomes depending on the species, the time of COC incubation with cilostamide, the combination with other agents and the experimental approach itself. In some cases substantial differences exist among treatments that it is difficult to compare their efficacy.…”

Section: Discussionsupporting

confidence: 89%

“…Many of these strategies, often referred to as 'prematuration culture', are based on the temporary blockage of oocyte spontaneous meiotic resumption, by modulating intra-oocyte cAMP content with different agents such as cAMP analogues or PDE inhibitors [12,16,[20][21][22][55][56][57][58][59][60][61][62][63][64][65][66][67]. Our results on the effect of the use of PDE inhibitors on meiotic resumption are largely supportive of previous data in pre-pubertal gilts in which cilostamide or other PDE3 inhibitors, such as milrinone, have been used [17,67,68].…”

Section: Discussionsupporting

confidence: 87%

“…Therefore fully-grown oocytes at advanced stages of differentiation would not benefit from protracted period of in vitro culture [29,70]. As a consequence, pre-maturation culture with cilostamide generally improves the yield (% of embryo obtained) of oocytes at early stages of their development [21,22,56,62], while it increase the quality of the embryos obtained from oocytes at more advanced stages of their differentiation, collected from mid-sized antral follicles, without a significant increase of the embryo rate [20,65,71]. Accordingly, in the present study similar outcomes on the quality of SCNT embryos are reported.…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Cilostamide on Gap Junction Communication Dynamics, Chromatin Remodeling, and Competence Acquisition in Pig Oocytes Following Parthenogenetic Activation and Nuclear Transfer1

Dieci

Lodde

Franciosi

et al. 2013

Biology of Reproduction

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In the pig, the efficiency of in vitro embryo production and somatic cell nuclear transfer (SCNT) procedures remains limited. It has been suggested that prematuration treatments (pre-IVM) based on the prolongation of a patent, bidirectional crosstalk between the oocyte and the cumulus cells through gap junction mediate communication (GJC), with the maintenance of a proper level of cAMP, could improve the developmental capability of oocytes. The aim of this study was to assess: 1) dose-dependent effects of cilostamide on nuclear maturation kinetics, 2) the relationship between treatments on GJC functionality and large-scale chromatin configuration changes, and 3) the impact of treatments on developmental competence acquisition after parthenogenetic activation (PA) and SCNT. Accordingly, cumulus-oocyte complexes were collected from 3- to 6-mm antral follicles and cultured for 24 h in defined culture medium with or without 1 μM cilostamide. GJC functionality was assessed by Lucifer yellow microinjection, while chromatin configuration was evaluated by fluorescence microscopy after nuclear staining. Cilostamide administration sustained functional coupling for up to 24 h of culture and delayed meiotic resumption, as only 25.6% of cilostamide-treated oocytes reached the pro-metaphase I stage compared to the control (69.7%; P < 0.05). Moreover, progressive chromatin condensation was delayed before meiotic resumption based upon G2/M biomarker phosphoprotein epitope acquisition using immunolocalization. Importantly, cilostamide treatment under these conditions improved oocyte developmental competence, as reflected in higher blastocyst quality after both parthenogenetic activation and SCNT.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

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The Effect of Cilostamide on Gap Junction Communication Dynamics, Chromatin Remodeling, and Competence Acquisition in Pig Oocytes Following Parthenogenetic Activation and Nuclear Transfer1

Dieci

Lodde

Franciosi

et al. 2013

Biology of Reproduction

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“…In induced IVM systems, GVBD is typically delayed (but not inhibited) by several hours; for example, from 1 to 3 h in mouse or from 6-7 h to 10-12 h in bovine (Thomas et al 2004a, Albuz et al 2010. Delay in time to MII depends on the type, dose and combination of cAMP modulators, but MII is either not delayed (Kim et al 2008) or delayed by only several hours (Thomas et al 2004b, Albuz et al 2010, Rose et al 2013. However, as GVBD is always delayed, the GVBD to MII interval is commonly shortened using induced or biphasic IVM systems, relative to standard IVM (Thomas et al 2004b, Kim et al 2008.…”

Section: Kinetics Of Meiosismentioning

confidence: 99%

Oocyte maturation and quality: role of cyclic nucleotides

Gilchrist¹,

Luciano²,

Richani³

et al. 2016

Reproduction

173

117

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The cyclic nucleotides, cAMP and cGMP, are the key molecules controlling mammalian oocyte meiosis. Their roles in oocyte biology have been at the forefront of oocyte research for decades, and many of the long-standing controversies in relation to the regulation of oocyte meiotic maturation are now resolved. It is now clear that the follicle prevents meiotic resumption through the actions of natriuretic peptides and cGMP -inhibiting the hydrolysis of intra-oocyte cAMP -and that the pre-ovulatory gonadotrophin surge reverses these processes.

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“…These culture systems (namely prematuration systems) that precede the in vitro maturation [10], are based on the control of spontaneous meiosis resumption through the addition of either cAMP analogues or adenylate cyclase activator or PDE inhibitors (specific or general) or through a combination of these treatments [15][16][17][18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Role of gap junction-mediated communications in regulating large-scale chromatin configuration remodeling and embryonic developmental competence acquisition in fully grown bovine oocyte

Lodde

Franciosi

Tessaro

et al. 2013

J Assist Reprod Genet

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Purpose This study was aimed to test the hypothesis that gap junction mediated communications (GJC) are required to allow the progressive chromatin configuration remodeling (from GV1 to GV3) process to occur in fully grown oocytes in order to gain the final step of developmental competence acquisition, and that a premature disruption of GJC can alter this process. Methods Bovine cumulus-oocytes complexes collected from medium antral follicles were cultured for 2, 4, 6 and 8 h in the presence of 10 −4 IU/ml of r-hFSH and with 2 mM of the nonselective PDE inhibitor 3-isobutyl-1-methyl-xanthine (IBMX) to prevent meiotic resumption. GJC functionality and chromatin configuration were monitored during the culture period. After meiotic arrest, the developmental capability of oocytes was assessed after IVM and IVF. Results IBMX was effective in significantly sustaining GJC up to 6 h and maintaining meiotic arrest, when compared to control group. Moreover, the percentage of oocytes with less condensed chromatin (GV1) decreased within 4 h of culture, while the proportion of GV2 oocytes gradually increased up to 6 h.Interestingly, a decline in the proportion of GV2 oocytes and an increase in the proportion of GV3 oocytes were observed after 6 h of culture, when the major drop of GJC occurred. On the contrary, when GJC were uncoupled by adding 3 mM of 1-heptanol or through cumulus cells removal, chromatin condensation occurred rapidly throughout the culture period, more promptly in denuded oocytes. Moreover, the maintenance of GJC during meiotic arrest was accompanied by a significant increase of developmental competence compared to the control, as indicated by a higher percentage of hatched blastocysts and blastocyst cell number. Conclusions Altogether, our data indicate that both paracrine and junctional mechanisms are involved in modulating largescale chromatin structure during the final phase of oocyte differentiation.

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Regulation of sheep oocyte maturation using cAMP modulators

Cited by 73 publications

References 47 publications

The Effect of Cilostamide on Gap Junction Communication Dynamics, Chromatin Remodeling, and Competence Acquisition in Pig Oocytes Following Parthenogenetic Activation and Nuclear Transfer1

The Effect of Cilostamide on Gap Junction Communication Dynamics, Chromatin Remodeling, and Competence Acquisition in Pig Oocytes Following Parthenogenetic Activation and Nuclear Transfer1

Oocyte maturation and quality: role of cyclic nucleotides

Role of gap junction-mediated communications in regulating large-scale chromatin configuration remodeling and embryonic developmental competence acquisition in fully grown bovine oocyte

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