The influence of bovine growth hormone and growth hormone releasing factor on acetyl-CoA carboxylase and fatty acid synthase in primiparous Holstein cows

Beswick, N.; Kennelly, J. J.

doi:10.1016/s0742-8413(98)10002-6

Cited by 16 publications

(10 citation statements)

References 38 publications

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“…This led to the assumption that there would be an increase in de novo fatty acid synthesis and increased uptake of preformed fatty acids from the blood into the mammary gland, in order to meet the increased substrate requirements for milk fat synthesis. Our previous analysis of lipogenic enzymes (Beswick and Kennelly, 1998) suggests that de novo synthesis in the mammary gland is not altered by either bGH or bGRF treatment. Thus, the enhanced milk fat synthesis may in part be accounted for by increased mammary parenchymal weight per 100 kg of carcass weight and increased total RNA content of mammary tissue.…”

Section: Resultsmentioning

confidence: 94%

Influence of bovine growth hormone and growth hormone-releasing factor on messenger RNA abundance of lipoprotein lipase and stearoyl-CoA desaturase in the bovine mammary gland and adipose tissue.

Beswick

Kennelly

2000

Journal of Animal Science

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Our objective was to determine the influence of bovine growth hormone (bGH) and bovine growth hormone-releasing factor (bGRF) administration on the mRNA abundance of lipoprotein lipase (LpL) and stearoyl-CoA desaturase (SCD). Primiparous Holstein cows received bGH, bGRF, or no treatment from 118 to 181+/-1 d postpartum. We hypothesized that bGH and bGRF treatment would increase the mRNA abundance of both SCD and LpL in the mammary gland with a corresponding reduction in adipose tissue. Milk yield significantly increased but milk fat percentage did not change as a result of bGH or bGRF treatment. Short-, medium-, and long-chain fatty acid concentrations in milk were not affected by either bGH or bGRF treatments, with the exception of a modest, but significant, increase in C16:1 and C18:1 following bGH treatment. Analysis was conducted on the genes encoding LpL (E.C. 3.3.1.34), a key enzyme involved in the uptake of fatty acids into tissues, and SCD (E.C. 1.14.99.5), which is the enzyme responsible for introducing delta9 double bonds in fatty acids of 16 and 18 carbons in length. In adipose tissue, treatment with bGH and bGRF reduced the mRNA abundance of LpL to 14.6 and 25.7% respectively, of that observed for control animals. Similarly, these treatments reduced the SCD mRNA abundance to undetectable levels in adipose tissue. In mammary gland, bGH and bGRF had no significant impact on LpL mRNA abundance. Bovine GH did not significantly affect SCD mRNA abundance in the mammary gland, and bGRF reduced SCD mRNA abundance. From this study to examine the role of bGH and bGRF on the expression of the genes encoding these key lipogenic enzymes in cattle, we conclude that the increased substrate required for enhanced milk fatty acid yield may have been provided through redirection of nutrients to the mammary gland away from adipose tissue and through overall increased metabolism in the mammary gland.

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Section: Resultsmentioning

confidence: 94%

Influence of bovine growth hormone and growth hormone-releasing factor on messenger RNA abundance of lipoprotein lipase and stearoyl-CoA desaturase in the bovine mammary gland and adipose tissue.

Beswick

Kennelly

2000

Journal of Animal Science

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“…A possible reason could be that there was less substrate in media provided for FA synthesis, thus, the addition of hormone to the cell culture provided was insufficient to increase flux through the pathway. In previous studies, Beswick [40] analysed the ACACA mRNA and protein abundance in the mammary gland of Holstein cows receiving either bovine growth hormone or bovine growth hormone-releasing factor, and revealed that there was no significant influence of the hormone alone. However, the transcription level of BTN1A1 and CSN2 suggested that bioactive factors including hormone in induction media could promote upregulation of secretory protin genes.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Culture and Characterization of a Mammary Epithelial Cell Line from Chinese Holstein Dairy Cow

Wang

et al. 2009

PLoS ONE

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BackgroundThe objective of this study was to establish a culture system and elucidate the unique characteristics of a bovine mammary epithelial cell line in vitro.MethodologyMammary tissue from a three year old lactating dairy cow (ca. 100 d relative to parturition) was used as a source of the epithelial cell line, which was cultured in collagen-coated tissue culture dishes. Fibroblasts and epithelial cells successively grew and extended from the culturing mammary tissue at the third day. Pure epithelial cells were obtained by passages culture.Principal FindingsThe strong positive immunostaining to cytokeratin 18 suggested that the resulting cell line exhibited the specific character of epithelial cells. Epithelial cells cultured in the presence of 10% FBS, supraphysiologic concentrations of insulin, and hydrocortisone maintained a normal diploid chromosome modal number of 2n = 60. Furthermore, they were capable of synthesizing β-casein (CSN2), acetyl-CoA carboxylase-α (ACACA) and butyrophilin (BTN1A1). An important finding was that frozen preservation in a mixture of 90% FBS and 10% DMSO did not influence the growth characteristics, chromosome number, or protein secretion of the isolated epithelial cell line.ConclusionsThe obtained mammary epithelial cell line had normal morphology, growth characteristics, cytogenetic and secretory characteristics, thus, it might represent an useful tool for studying the function of Chinese Holstein dairy cows mammary epithelial cell (CMECs).

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“…VANDERKOOI et al (1995) reported that bGHRH stimulates milk synthesis through the same mechanisms as bGH. BESWICK and KENNELLY (1998), who measured mRNA and protein abundance of the acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS) in the mammary gland and adipose tissue, presented a potential role of GHRH in the lipid metabolism of the mammary gland.…”

Section: Introductionmentioning

confidence: 99%

<i>GHRH/Hae</i>III gene polymorphism and its associations with milk production traits in Polish Black-and-White cattle (short communication)

Dybus

Grzesiak

2006

Arch. Anim. Breed.

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Abstract. The objective of this paper was to evaluate the relationship between the polymorphism of the GHRH and milk production traits of Polish Black-and-White. A total of 881 cows were included in the study. A PCR-RFLP method was used to genotyping. The frequencies of the genotypes and alleles were as follows: 0.0545 for AA, 0.3133 for AB and 0.6322 for BB, and 0.2111 for GHRHA and 0.7889 – GHRHB. There were no significant associations between GHRH/HaeIII polymorphism and milk production traits of the analysed cows.

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The influence of bovine growth hormone and growth hormone releasing factor on acetyl-CoA carboxylase and fatty acid synthase in primiparous Holstein cows

Cited by 16 publications

References 38 publications

Influence of bovine growth hormone and growth hormone-releasing factor on messenger RNA abundance of lipoprotein lipase and stearoyl-CoA desaturase in the bovine mammary gland and adipose tissue.

Influence of bovine growth hormone and growth hormone-releasing factor on messenger RNA abundance of lipoprotein lipase and stearoyl-CoA desaturase in the bovine mammary gland and adipose tissue.

In Vitro Culture and Characterization of a Mammary Epithelial Cell Line from Chinese Holstein Dairy Cow

<i>GHRH/Hae</i>III gene polymorphism and its associations with milk production traits in Polish Black-and-White cattle (short communication)

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The influence of bovine growth hormone and growth hormone releasing factor on acetyl-CoA carboxylase and fatty acid synthase in primiparous Holstein cows

Cited by 16 publications

References 38 publications

Influence of bovine growth hormone and growth hormone-releasing factor on messenger RNA abundance of lipoprotein lipase and stearoyl-CoA desaturase in the bovine mammary gland and adipose tissue.

Influence of bovine growth hormone and growth hormone-releasing factor on messenger RNA abundance of lipoprotein lipase and stearoyl-CoA desaturase in the bovine mammary gland and adipose tissue.

In Vitro Culture and Characterization of a Mammary Epithelial Cell Line from Chinese Holstein Dairy Cow

&lt;i&gt;GHRH/Hae&lt;/i&gt;III gene polymorphism and its associations with milk production traits in Polish Black-and-White cattle (short communication)

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<i>GHRH/Hae</i>III gene polymorphism and its associations with milk production traits in Polish Black-and-White cattle (short communication)