The influence of age on the DNA ploidy levels of breast tumours

Taylor, Ian W.; Musgrove, Elizabeth A.; Friedlander, Michael; Foo, Ming San; Hedley, David W.

doi:10.1016/0277-5379(83)90178-5

Cited by 57 publications

(23 citation statements)

References 12 publications

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“…'.^-^,^,' 1,16,22,28 In this study a good correlation was also found between the grade of differentiation of breast carcinomas and DNA content abnormalities. In estimating the grade of differentiation of breast carcinomas we have introduced a grading system including variety of histologic and cytologic criteria: tumor infiltration into the surrounding tissues and blood vessels, heterogeneity in the arrangement of tumor cells, presence of necrotic area and calcifications among tumor parenhyma, and cytologic pleomorphysm.…”

supporting

confidence: 64%

DNA aneuploidy and cell proliferation in breast tumors

Christov¹,

Milev²,

Todorov³

1989

Cancer

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The cellular DNA content of 30 benign and 180 malignant breast tumors was analyzed by means of flow cytometry (FCM). A11 benign tumors exhibited a normal DNA content (diploid), whereas 65% of the malignant tumors showed an abnormal DNA content (aneuploid). The ploidy distribution of malignant tumors was bimodal with an increasing frequency near diploid DNA index (DI), and a second group had a DI ranging from triploid to tetraploid. In estimating the degree of malignancy eight independent histomorphologic and cytologic criteria were introduced. A good correlation was observed between DNA content abnormalities and the grade of differentiation of breast carcinomas. The percentage of S-phase

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supporting

confidence: 64%

DNA aneuploidy and cell proliferation in breast tumors

Christov¹,

Milev²,

Todorov³

1989

Cancer

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“…The amount of elastin and the extent of necrosis have been associated with histological grade too (Fisher et al, 1984;Kuhajda et al, 1985). The association of ploidy with primary tumour size and axillary nodal status has been controversial (Uyterlinde et al, 1988;Spyratos et al, 1987;Taylor et al, 1983;McDivitt et al, 1986;Cornelisse et al, 1987;Dressler et al, 1988). In this series DNA aneuploidy was significantly more common in large primary tumours, and in tumours with axillary or distant metastases (Table II).…”

Section: Discussionmentioning

confidence: 67%

“…This has also been noticed by Taylor et al (1983). DNA aneuploid tumours usually have hypertriploid DNA content, and it has been suggested that DNA aneuploidy develops via tetra-ploidy: tetraploid tumours lose some of their chromosomal material resulting in hypertriploid nuclear DNA content (Ewers et al, 1984).…”

Section: Discussionmentioning

confidence: 93%

The prognostic significance of nuclear DNA content in invasive breast cancer – a study with long-term follow-up

Toikkanen

Joensuu²,

Klemi³

1989

Br J Cancer

117

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Summary The nuclear DNA content of 351 breast carcinomas was determined by flow cytometry from paraffin-embedded tissue to assess the prognostic significance of DNA ploidy, the DNA index (DI) and the S-phase fraction (SPF). The minimum follow-up of the patients was 22 years, and they were all from a defined urban population. DNA ploidy correlated with histological type and grade, mitotic count and nuclear pleomorphism (P<0.0001), and also with axillary nodal status (P=0.0005), tumour necrosis (P=0.001), primary tumour size (P = 0.03), menopausal status (P = 0.004) and the presence of distant metastases at the time of the diagnosis (P = 0.04). Survival corrected for intercurrent deaths of the patients with a diploid tumour was better than that of the patients with a non-diploid tumour (P = 0.0001, 48% vs 28% at 25 years). SPF Leatham & Brooks, 1987; Slamon et al., 1987).The nuclear DNA content determined by flow cytometry has recently emerged as a new prognostic factor in breast cancer. The technique has gained some popularity, since flow cytometric histograms are rapid to produce and often easy to interpret. It has been suggested that breast carcinomas with an abnormal (non-diploid or aneuploid) nuclear DNA content are associated with less favourable prognosis than carcinomas with a normal (diploid) DNA content (Hedley et al., 1984(Hedley et al., , 1987Coulson et al., 1984;Ewers et al., 1984;Thorud et al., 1986;Baildam et al., 1987;Cornelisse et al., 1987;Kallioniemi et al., 1987;Stal et al., 1989). Similarly, the DNA index (DI, the relative DNA content of an aneuploid stemline of cells as compared with diploid cells) and the percentage of S-phase cells in the DNA histogram (S-phase fraction, SPF) have been found by some to be of prognostic significance (Dowle et al., 1987;Hedley et al., 1987;Kallioniemi et al., 1988;Stal et al., 1989;McDivitt et al., 1986;Klintenberg et al., 1986). However, the prognostic value of nuclear DNA content analysis is still unsettled. Some authors report DNA content to be the most important prognostic variable in breast cancer, and to have independent prognostic value in a multivariate analysis , whereas others find it to have prognostic value only in a univariate analysis (Stal et al., 1989;Hedley et al., 1984), and yet a few fail to find any prognostic significance at all when overall survival is concerned (van der Linden et al., 1989;Uyterlinde et al., 1988;Owainati et al., 1987

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“…A statistically significant association between the presence of aneuploid tumour nuclei and the absence of ER is apparent from some studies (Auer et al, 1980; Bichel et al, 1982;Olszewski et al, 1981), but in others only a trend was observed (Cornelisse et al, 1984;Kute et al, 1981;Raber et al, 1982;Taylor et al, 1983). We were thus prompted to re-examine this association.…”

Section: Discussionmentioning

confidence: 97%

“…In general, these studies have shown that variables associated with poor prognosis in breast cancer appear to be associated more frequently with an absence of oestrogen receptors. While the presence of aneuploid tumour nuclei has been linked to poor prognosis (Atkin, 1972;Auer et al, 1984), an association with oestrogen receptor content has not been conclusively established (Auer et al, 1980;Bichel, et al, 1982;Cornelisse et al, 1984 al., Olszewski et al, 1981;Raber et al, 1982;Taylor et al, 1983). One of these studies (Bichel et al, 1982) suggested that aneuploidy might be responsible for the lack of endocrine responsiveness which occurs in 40% of ER positive tumours.…”

mentioning

confidence: 99%