Relationship between ploidy and steroid hormone receptors in primary invasive breast cancer

Horsfall, Debbie; Tilley, Wayne D.; Orell, SR; Marshall, Villis R.; Cant, E. L. M.

doi:10.1038/bjc.1986.4

Cited by 68 publications

(29 citation statements)

References 18 publications

(22 reference statements)

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“…Clinical stage (UICC) was also positively correlated with aneuploidy incidence in their study, but in our series of patients no statistically significant trend was found. Several authors (3,17,20,24) have reported a n inverse correlation between ER status and ploidy where others have not found such a relationship (6,10,16,25). In the present study a simificant correlation was found only with more recently determined ER values after increasing the sensitivity of the assay.…”

Section: Discussionsupporting

confidence: 36%

DNA ploidy and survival in breast cancer patients

et al. 1987

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Flow cytometric DNA ploidy measurements using frozen or deparaffinized tumor specimens were performed on 565 primary breast cancers from patients treated in the period [1975][1976][1977][1978][1979][1980][1981][1982][1983][1984]. Twenty-nine percent of the cases were diploid, 61% had a single aneuploid stemline, and 10% were multiploid. Aneuploid tumors more often had negative estrogen receptor values than diploid tumors, but no significant correlation was found between ploidy class and TNM stage. Patients with more than ten positive axillary lymph nodes had predominantly aneuploid tumors, Overall and distant relapse-free survival were higher for patients with diploid tumors and low-aneuploid tumors. Stratification of the patients according to degree of lymph node involvement, TNM stage, and menopausal stage showed that the prognostic effect of aneuploidy was apparent predominantly in patients with locally advanced disease. Postmenopausal node-positive patients with diploid tumors had a significantly better prognosis than those with aneuploid tumors, but this difference was not found for the comparable premenopausal group. Multivariate analysis with the Cox proportional hazards model indicated that ploidy is an additional, independent prognostic factor in postmenopausal patients.

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Section: Discussionsupporting

confidence: 36%

DNA ploidy and survival in breast cancer patients

et al. 1987

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“…The breast tumor tissue samples were graded according to the Elston and Ellis modification of the Bloom and Richardson grading system (13). Steroid receptor status was routinely assessed using the radioligand binding assay (14). Clinical and pathological data for the cohort is summarized in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Expression of Extracellular Matrix Components Versican, Chondroitin Sulfate, Tenascin, and Hyaluronan, and Their Association with Disease Outcome in Node-Negative Breast Cancer

Suwiwat

Ricciardelli

Tammi

et al. 2004

Clinical Cancer Research

136

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Purpose:The purpose is to determine whether the levels of expression of extracellular matrix components in peritumoral stroma are predictive of disease outcome for women with node-negative breast cancer.Experimental Design: Tumor tissue from 86 patients with node-negative breast cancer was examined by immunohistochemical staining for the expression of versican, chondroitin sulfate (CS), tenascin, and hyaluronan (HA). With the exception of HA, the expression of the extracellular matrix components was measured by video image analysis. Statistical correlation of the immunohistochemical data with clinicopathological characteristics and disease outcome was performed.Results: All of the extracellular matrix components were present in the peritumoral stroma of the entire study cohort. In contrast, immunoreactivity within the cancer cell was observed in 82% of tumors for HA, 12% for CS, and 4% for tenascin; no immunostaining of cancer cells for versican was observed for any of the tumors. Cox regression and Kaplan-Meier analyses indicated that elevated expression of stromal versican predicted increased risk and rate of relapse in this cohort. Elevated expression of tenascin was predictive of increased risk and rate of death only. Although neither CS nor HA were predictive of disease outcome in this cohort, tumor size was predictive of increased risk and rate of both relapse and survival.Conclusions: Elevated expression within peritumoral stromal matrix of versican and tenascin was predictive of relapse-free and overall survival, respectively, in women with node-negative breast cancer.

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“…There is a consistent trend for a greater proportion of diploid tumours to be oestrogen receptor positive when compared to aneuploid tumours; this difference achieves statistical significance in some studies (Bichel et al, 1982;Cornelisse et al, 1984;Coulson et al, 1984;Horsfall et al, 1986) but not in others (Olszewski et al, 1981a;Raber et al, 1982;Taylor et al, 1983;Hedley et al, 1984;McDivitt et al, 1985;Kute et al, 1985).…”

mentioning

confidence: 99%

“…Two thirds (range 54-92%) of human mammary tumours have been reported to be aneuploid (Olszewski et al, 1981a;Raber et al, 1982;Cornelisse et al, 1984;Ewers et al, 1984;Moran et al, 1984;Hedley et al, 1984Hedley et al, , 1985McDivitt et al, 1986;Thorud et al, 1986;Horsfall et al, 1986).…”

mentioning

confidence: 99%

DNA analysis by flow cytometry, response to endocrine treatment and prognosis in advanced carcinoma of the breast

Baildam¹,

Žaloudík²,

Howell³

et al. 1987

Br J Cancer

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Summary The relationship between DNA content of mammary cancer and subsequent response to endocrine therapy was studied in 136 patients with advanced disease. All were treated with tamoxifen or ovarian ablation as first-line systemic therapy after relapse and were evaluable for response according to UICC criteria. DNA characterisation by flow cytometry was used on formalin fixed paraffin-embedded samples of tumour. Tumours were grouped according to DNA index into diploid (n = 52, 38%), 'tetraploid' (n =46, 34%) and 'other DNA-aneuploid' (n=38, 28%). The highest proportion of oestrogen receptor positive tumours (ER+ve) was found in the 'tetraploid' tumours (38/46, 85%, Chi-square=8.53, P<0.02), and response rates, (SD + PR+CR), were 26/52 (50%), 34/46 (74%), and 15/38 (39%) respectively (Chi-square= 10.88, P<0.005). Patients with diploid or 'tetraploid' tumours survived longer and stayed in remission longer than those with other DNA-aneuploid' tumours. We suggest that 'tetraploid' or 'near tetraploid' human mammary tumours may comprise a distinct group of endocrine responsive tumours within the overall group of aneuploid tumours. The conventional interpretation of DNA histograms, grouping into diploid and aneuploid, may be masking important features of some tumour groups.In the past cellular DNA ploidy was assessed by the use of nucleic acid specific stains on tissue sections and microdensitometry. Only relatively small numbers of cells could be assessed. The development of flow cytometry (FCM) has brought a rapid and reproducible technique for determination of DNA content of tumours. Thousands of nuclei can be evaluated, and DNA histograms produced which indicate different cell populations.

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Relationship between ploidy and steroid hormone receptors in primary invasive breast cancer

Cited by 68 publications

References 18 publications

DNA ploidy and survival in breast cancer patients

DNA ploidy and survival in breast cancer patients

Expression of Extracellular Matrix Components Versican, Chondroitin Sulfate, Tenascin, and Hyaluronan, and Their Association with Disease Outcome in Node-Negative Breast Cancer

DNA analysis by flow cytometry, response to endocrine treatment and prognosis in advanced carcinoma of the breast

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