The inflammatory potential of biphasic calcium phosphate granules in osteoblast/macrophage co-culture

Curran, Judith M.; Gallagher, James A.; Hunt, John A.

doi:10.1016/j.biomaterials.2005.01.065

Cited by 55 publications

(38 citation statements)

References 42 publications

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“…Small HA particles 1-3 lm in diameter tend to biodegrade in rabbit bone marrow [35]. The dissolution characteristics of calcium phosphate materials could be the stimulation of inflammatory responses by cells in charge of immune responses [46]. It is still unclear whether the dissolution of OCP affects the osteoclastic activity around OCP and the subsequent bone formation on it; however, the subtle change in the stoichiometry of OCP (Ca/P molar ratio from 1.26 to 1.37 compared to the stoichiometric 1.33 of OCP) has been confirmed to raise the osteoconductivity and reduce its own biodegradation at the initial stage of implantation by osteoclastic resorption, accompanied by the inhibition of inflammatory responses [31].…”

Section: Discussionmentioning

confidence: 99%

Comparative study on bone regeneration by synthetic octacalcium phosphate with various granule sizes

et al. 2010

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Section: Discussionmentioning

confidence: 99%

Comparative study on bone regeneration by synthetic octacalcium phosphate with various granule sizes

et al. 2010

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“…To differentiate TNF-a, IL-6 and IL-1b responses at early and late stages, the mRNA expression levels of these cytokines were analyzed at 6 and 24 h post-treatment (at the times selected for the analysis of cell-powder interaction [34]. Based on the obtained mRNA expression data, protein levels were measured after 24 h of treatment [35]; thus, the macrophages interacted with the surfaces over a sufficient period [36].…”

Section: Discussionmentioning

confidence: 99%

Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Chen

Liu

Wei

et al. 2016

J Mater Sci: Mater Med

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Inflammatory effects are significant elements of the immune response to biomaterials. Previously, we reported inflammatory effects in response to dicalcium silicate (Ca2SiO4, C2S) particles. However, the immunological effects of C2S coatings have not been studied. C2S often used as coatings materials in orthopedic and dentistry applications. It may have different effect from C2S particles. Further, it remains unclear whether C2S coating is equally biocompatible as 45S5 coating. The aim of this study was to test the cytotoxicity and pro-inflammatory effects of C2S coating on RAW 264.7 macrophages. C2S and 45S5 coatings were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), energy dispersive analysis (EDS) and X-ray diffraction (XRD). inductively coupled plasma optical emission spectroscopy (ICP-OES) was used to detect ionic concentrations after soaking coated discs in medium. The cytotoxicity of C2S and 45S5 coatings against RAW 264.7 macrophages was measured using the LDH Cytotoxicity Assay Kit, Cell Counting Kit-8 (CCK-8) assays and flow cytometry for apoptosis assays. The gene and protein expression of TNF-α, IL-6 and IL-1β were detected using RT-q PCR and ELISA, respectively. The tested coating materials are not cytotoxic to macrophages. The C2S-coated surface stimulated macrophages to express pro-inflammatory mediators, such as TNF-α, IL-6 and IL-1β, and C2S coating caused less IL-6 but greater IL-1β production than the 45S5 coating. C2S coating have no cytotoxicity when directly cultured with macrophages. C2S and 45S5 coatings both have the potential to induce pro-inflammatory effects, and the biocompatibility of C2S is similar to that of 45S5.

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“…-tricalcium phosphate ( -TCP, -Ca 3 (PO 4 ) 2 ) is receiving growing attention as a raw material for several injectable hydraulic bone cements, biodegradable bioceramics and composites for bone repair 8) . -TCP particles have outstanding bone regeneration ability, but the -TCP material tends to remain in the defect, which can lead to severe inflammatory response or delaying bone healing [9][10][11] .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Bone Regeneration by porous Alpha-Tricalcium Phosphate/Atelocollagen Sponge Composite in Critical-Sized Rat Calvarial Defects

Hashimoto

Honda

et al. 2016

J. Hard Tissue Biology.

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In periodontal surgery, autogenous bones are frequently used for bone transplantation. However, the application of autogenous bone is limited by the lack of sufficient donor sites and high possibility of resorption. In this study, we attempted to use the -tricalcium phosphate/atelocollagen sponge composite ( -TCP/CS) to repair rat calvarial defects compared with that using autogenous bone. For the experiments, rats were divided into three groups. One group was filled with -TCP/CS. Another was filled with autogenous bone, and control group remained unfilled. The rats were euthanized at 4, 6, and 8 weeks after surgery, after which the calvarial bone was scanned by micro-CT, and the histological examination was performed for evaluation of the bone tissue structures. In vivo micro-CT images at 8 weeks after surgery revealed that, in the -TCP/CS group, the regenerative bone area was larger than in the autogenous bone group. Histological analysis revealed that -TCP/CS was mostly absorbed at 8 weeks and a contiguous new bone was observed. This study demonstrated that composite sponges created from porous -TCP/CS composite particles and atelocollagen were adaptable for treating bone defects.

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The inflammatory potential of biphasic calcium phosphate granules in osteoblast/macrophage co-culture

Cited by 55 publications

References 42 publications

Comparative study on bone regeneration by synthetic octacalcium phosphate with various granule sizes

Comparative study on bone regeneration by synthetic octacalcium phosphate with various granule sizes

Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Evaluation of Bone Regeneration by porous Alpha-Tricalcium Phosphate/Atelocollagen Sponge Composite in Critical-Sized Rat Calvarial Defects

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