The induction of oxidative stress, cytotoxicity, and genotoxicity by dental adhesives

Demırcı, Mustafa; Hiller, Karl‐Anton; Bosl, C.; Galler, Kerstin M.; Schmalz, Gottfried; Schweikl, Helmut

doi:10.1016/j.dental.2007.06.009

Cited by 125 publications

(124 citation statements)

References 40 publications

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“…Although some very encouraging fi ndings have been published (39,40), it seems that dental adhesives may be too toxic for direct pulp capping (14)(15)(16)(17)(18)(19)41), as due to their water solubility they gradually release potentially toxic resin components: HEMA and TEGDMA. Both of them are capable to induce apoptosis or necrosis in normal human primary cells.…”

Section: Resultsmentioning

confidence: 99%

The Effects of Hyaluronic Acid, Calcium Hydroxide, and Dentin Adhesive on Rat Odontoblasts and Fibroblasts

Bogović

Nižetić

Galić

et al. 2011

Archives of Industrial Hygiene and Toxicology

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The Effects of Hyaluronic Acid, Calcium Hydroxide, and Dentin Adhesive on Rat Odontoblasts and FibroblastsThe aim of this study was to investigate the effects and efficiency of pulp capping preparations based on hyaluronic acid, calcium hydroxide, and dentin adhesive on the pulp tissue of Sprague-Dawley rats. The rats were killed and extracted teeth sectioned transversely through the pulp. The slices were placed in a RPMI 1640 cell culture medium supplemented with 10 % foetal calf serum. During 14 days of cultivation cultures were treated with preparations that contained hyaluronic acid (Gengigel Prof®), and calcium hydroxide (ApexCal®), or with dentin adhesive (Excite®). Cellularity and viability of fibroblasts and odontoblasts was analysed using a haemocytometer. Hyaluronic acid proved most efficient and the least toxic for direct pulp capping. Even though calcium hydroxide and dentin adhesive demonstrated a higher degree of cytotoxicity, their effects were still acceptable in terms of biocompatibility.

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Section: Resultsmentioning

confidence: 99%

The Effects of Hyaluronic Acid, Calcium Hydroxide, and Dentin Adhesive on Rat Odontoblasts and Fibroblasts

Bogović

Nižetić

Galić

et al. 2011

Archives of Industrial Hygiene and Toxicology

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“…Resinbased dentine bonding agents were also found to reduce intracellular glutathione 17) . Their cytotoxicity effects were prevented with antioxidants 18,19) , suggesting that resin monomers play a role in the cytotoxicity of bonding agents. Eluting resin monomers from resinbased materials generally depends on the degree of polymerization.…”

Section: Discussionmentioning

confidence: 96%

The effects of restorative composite resins on the cytotoxicity of dentine bonding agents

et al. 2013

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During restoration of damaged teeth in dental clinics, dentin bonding agents are usually overlaid with restorative resin composites. The purpose of this study was to investigate the effects of restorative resin composites on cytotoxicity of dentin bonding agents. Dentin bonding agents were placed on glass discs, pre-cured and uncured resin composite discs. Bonding agents on the glass discs and composite resins discs were light cured and used for agar overlay cytotoxicity testing. Dentin bonding agents on composite resin discs exhibited far less cytotoxicity than that on glass discs. The polymerization of resin composite increased the surface hardness and decreased the cytotoxicity of bonding agents. In conclusion, composite resins in dental restorations are expected to enhance the polymerization of dentin bonding agents and reduce the elution of resin monomers, resulting in the decrease of cytotoxicity.

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“…In the same study, two nano dentin bonding agents (Futurabond NR and Xeno III) induced G2/M arrest at higher concentrations, a finding that was concomitant with an increase in apoptosis. Other dentin bonding agents, such as Excite, Clearfil SE and Adhese SE bond have been found to induce cytotoxicity, genotoxicity and reactive oxygen species (ROS) production in pulp cells and in the V79 cell line 21) . Recent in vitro experiments have suggested that the total-etch adhesive XP bond, exerts strong antiproliferative effect in 3T3 mouse fibroblasts 22) .…”

Section: Discussionmentioning

confidence: 99%

Effects of a dental adhesive on cell cycle regulatory proteins

et al. 2013

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Dental bonding agents may affect the cell cycle patterns and induce cell cycle arrest by blocking its progression. This study tested the cell cycle effects through cyclin-dependent kinase (cdc2) and Rb phosphorylation. Human lung fibroblasts (MRC5) were used for the experiments. The bonding agent tested was the total-etch XP bond. Extracts of the bonding agent were prepared and serial dilutions were tested. The effects of the bonding agent on cell survival, proliferation and DNA synthesis were tested by the SRB and BrdU assays. Analysis of cell cycle distribution was performed by flow cytometry. XP bond exhibited strong inhibition of DNA synthesis and after 48 h of exposure cells were accumulated in the G2/M phase. Cells exposed to the half maximal cell growth inhibitory concentration (IC 50 ) showed an increase in cdc2 kinase and Rb phosphorylation. The results most likely indicate mutagenic effect of the tested agent.

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The induction of oxidative stress, cytotoxicity, and genotoxicity by dental adhesives

Cited by 125 publications

References 40 publications

The Effects of Hyaluronic Acid, Calcium Hydroxide, and Dentin Adhesive on Rat Odontoblasts and Fibroblasts

The Effects of Hyaluronic Acid, Calcium Hydroxide, and Dentin Adhesive on Rat Odontoblasts and Fibroblasts

The effects of restorative composite resins on the cytotoxicity of dentine bonding agents

Effects of a dental adhesive on cell cycle regulatory proteins

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