The Effects of Hyaluronic Acid, Calcium Hydroxide, and Dentin Adhesive on Rat Odontoblasts and Fibroblasts

Abstract

The Effects of Hyaluronic Acid, Calcium Hydroxide, and Dentin Adhesive on Rat Odontoblasts and FibroblastsThe aim of this study was to investigate the effects and efficiency of pulp capping preparations based on hyaluronic acid, calcium hydroxide, and dentin adhesive on the pulp tissue of Sprague-Dawley rats. The rats were killed and extracted teeth sectioned transversely through the pulp. The slices were placed in a RPMI 1640 cell culture medium supplemented with 10 % foetal calf serum. During 14 days of cultivation cultures were treated with preparations that contained hyaluronic acid (Gengigel Prof®), and calcium hydroxide (ApexCal®), or with dentin adhesive (Excite®). Cellularity and viability of fibroblasts and odontoblasts was analysed using a haemocytometer. Hyaluronic acid proved most efficient and the least toxic for direct pulp capping. Even though calcium hydroxide and dentin adhesive demonstrated a higher degree of cytotoxicity, their effects were still acceptable in terms of biocompatibility.

In vitro cytotoxicity evaluation of different pulp capping materials: a comparative study

“…In the last decade, many experimental and clinical studies have been carried out to develop and test new dental materials endowed with safe biocompatibility and antiinfective properties (26)(27)(28). The material used should provide an appropriate host response.…”

“…After washing the slides with Buffer-TES, 250 mL of 10 mmol L -1 solution of the fluorescent dye PSVue480™ was added per well, in order to detect the presence of apoptotic cells present in culture. The loss of plasma membrane asymmetry is an early event in apoptosis, independent of cell type, resulting in the exposure of phosphatidylserine (PS) residues at the outer plasma membrane leaflet (26). PSVue reagents are a family of fluorescent probes containing a bis (zinc 2+ dipicolylamine) group (Zn-DPA), a motif that has been found to bind with high affinity to surfaces enriched with anionic phospholipids, especially phosphatidylserine (PS) exposed on cell membranes.…”

New Trends in Dentin Bonding: Treatment with chlorhexidine, hyaluronic acid, vitaminc C and green tea

“…The wide applicability of HA in skin cosmetology is attributed to its excellent hygroscopic character, optimal rheological behavior, viscoelastic property and moisturizing effect, which may promote infiltration of adhesive resin monomers in the exposed collagen network of acid-etched dentin1. According to Bogovic et al (2011) [19], hyaluronic acid may be applied in direct pulp capping to stimulate vascularization, creating favorable conditions for mineralization and stimulating the proliferation and differentiation of odontoblasts. This agent also possesses antioxidant characteristics, neutralizing the harmful effects of free radicals, and preserves the structural integrity of tissues [31,32].…”

“…The hyaluronic acid is used to provide a moisturizing effect and act as an antioxidant. The vitamin C is used to increase the level of messenger RNA of collagen I and III, as a co-factor for the conversion of some enzymes, and as an inhibitor of MMPs [19][20][21].…”

Micronucleus, alkaline, and human 8-oxoguanine glycosylase 1 modified comet assays evaluation of glass-ionomer cements - in vitro

“…Dental materials can lead to cell damage of the pulp, gingiva, and oral mucosa. Various tests have been applied to assess the risk of such damage (1)(2)(3)(4).…”