The induction of delayed type hypersensitivity to dinitrochlorobenzene in the chicken

Huynh, V.; Chubb, R. C.

doi:10.1080/03079458708436389

Cited by 10 publications

(2 citation statements)

References 27 publications

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“…2.9 Assessment of cell-mediated and humoral immunity 2.9.1 Delayed type hypersensitivity (DTH) reaction assay In vivo, cell-mediated immune response was evaluated by assessing the DTH reaction to 2, 4-dinitro-1chlorobenzene (DNCB) dye by following an earlier standard method with minor modi cations [17]. Three cockerels from each group were arbitrarily selected and 2×2 cm 2 aseptically defeathered skin areas on the left and right abdomen of each cockerel were marked with India ink at day 50.…”

Section: Brain Acetylcholinesterasementioning

confidence: 99%

Subchronic Acephate Exposure Induces Immunotoxicity in White Leghorn Cockerels

Tripathi

Govindappa

Bedekar

et al. 2021

Preprint

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We investigated the subchronic immunotoxicity of the phosphoramidothioate organophosphorous insecticide, acephate in white leghorn cockerels (WLH). The cockerels were divided into five groups; C1 (plain control), C2 (vehicle control), T1, T2, and T3 which received acephate suspended groundnut oil for 60 days at doses of 21.3, 28.4 and 42.6 mgkg− 1respectively. The live body weight gain, absolute and relative weights of the spleen, thymus, and bursa of Fabricius, hemoglobin (Hb), total erythrocyte counts (TEC), packed cell volume (PCV) and lymphocytes were significantly decreased. However, monocytes, eosinophils, heterophils, and basophils were significantly increased. Total protein, albumin and albumin to globin ratio, the antibody response to RD-F and delayed-type hypersensitivity response to DNCB dye or PHA-P, erythrocyte and brain Acetylcholinesterase activity was also significantly reduced in T2 and T3. At 40 and 60 days of acephate exposure, nitrate and nitric oxide production by RD-F and mitogen Con A stimulated peripheral blood and splenic lymphocytes, as well as lymphocyte proliferation in response to antigen RD-F and mitogen Con A stimulation, were significantly decreased in groups T2 and T3. Furthermore, dose-dependent increases in the frequency of micronuclei formation, varying intensity serum protein bands with different protein fractions (14.85KDa), and splenic DNA laddering (180 bp) were observed in groups T2 and T3. Histopathologically, the spleen and bursa showed morphological changes and mild lymphocyte depletion. In conclusion, low-level acephate exposure may affect acetylcholinesterase, lymphocytes, and immune responses in cockerels. As a result, it should be considered when assessing immunotoxicity and the risk to human and animal health.

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Section: Brain Acetylcholinesterasementioning

confidence: 99%

Subchronic Acephate Exposure Induces Immunotoxicity in White Leghorn Cockerels

Tripathi

Govindappa

Bedekar

et al. 2021

Preprint

View full text Add to dashboard Cite

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“…This unresponsiveness could be abrogated by treatment with cytotoxic drugs such as cyclophosphamide (Askenase et al, 1975;Sy et al, 1977); aclacinomycin (Arbouys etal., 1983) and amphotericin B (Shirley and little, 1979). This paper evaluates the suppressed DTH reaction in chickens induced by supraoptimal sensitisation with DNCB (Huynh and Chubb, 1987) especially in relation to suppressor cells and the effects of cytotoxic drugs on it.…”

Section: Introductionmentioning

confidence: 99%