The induction of cytochrome P-450 inLodderomyces elongisporus

Mauersberger, Stephan; Schunck, Wolf‐Hagen; Müller, Hannelore

doi:10.1002/jobm.3630210407

Z Allg Mikrobiol

1981

DOI: 10.1002/jobm.3630210407

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The induction of cytochrome P-450 inLodderomyces elongisporus

Stephan Mauersberger¹,

Wolf‐Hagen Schunck²,

Hannelore Müller³

Abstract: In the alkane-utilizing yeast strain Lodderomyces elongisporus cytochrome P-450 is induced by aliphatic hydrocarbons and to a lesser degree also by some of their derivatives. Cycloheximide and glucose inhibit the induction process, the former by inhibition of cytoplasmic translation, the latter presumably by catabolite repression. Among the nearly 40 checked compounds tetradecane and 1-tetradecene are the most effective inducers. The branching of the alkyl chain as well as the terminal introduction of electrop… Show more

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Cited by 28 publications

References 14 publications

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Genome‐scale metabolic modeling and in silico analysis of lipid accumulating yeast Candida tropicalis for dicarboxylic acid production

Mishra

Park

Lee³

et al. 2016

Biotech & Bioengineering

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Recently, the bio-production of α,ω-dicarboxylic acids (DCAs) has gained significant attention, which potentially leads to the replacement of the conventional petroleum-based products. In this regard, the lipid accumulating yeast Candida tropicalis, has been recognized as a promising microbial host for DCA biosynthesis: it possess the unique ω-oxidation pathway where the terminal carbon of α-fatty acids is oxidized to form DCAs with varying chain lengths. However, despite such industrial importance, its cellular physiology and lipid accumulation capability remain largely uncharacterized. Thus, it is imperative to better understand the metabolic behavior of this lipogenic yeast, which could be achieved by a systems biological approach. To this end, herein, we reconstructed the genome-scale metabolic model of C. tropicalis, iCT646, accounting for 646 unique genes, 945 metabolic reactions, and 712 metabolites. Initially, the comparative network analysis of iCT646 with other yeasts revealed several distinctive metabolic reactions, mainly within the amino acid and lipid metabolism including the ω-oxidation pathway. Constraints-based flux analysis was, then, employed to predict the in silico growth rates of C. tropicalis which are highly consistent with the cellular phenotype observed in glucose and xylose minimal media chemostat cultures. Subsequently, the lipid accumulation capability of C. tropicalis was explored in comparison with Saccharomyces cerevisiae, indicating that the formation of "citrate pyruvate cycle" is essential to the lipid accumulation in oleaginous yeasts. The in silico flux analysis also highlighted the enhanced ability of pentose phosphate pathway as NADPH source rather than malic enzyme during lipogenesis. Finally, iCT646 was successfully utilized to highlight the key directions of C. tropicalis strain design for the whole cell biotransformation application to produce long-chain DCAs from alkanes. Biotechnol. Bioeng. 2016;113: 1993-2004. © 2016 Wiley Periodicals, Inc.

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Genome‐scale metabolic modeling and in silico analysis of lipid accumulating yeast Candida tropicalis for dicarboxylic acid production

Mishra

Park

Lee³

et al. 2016

Biotech & Bioengineering

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Biotechnological synthesis of long‐chain dicarboxylic acids as building blocks for polymers

Huf

Krügener

Hirth

et al. 2011

Eur. J. Lipid Sci. Technol.

119

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An important field in sustainable industrial chemistry is the development of new applications for fats and oils. One of the promising applications is the use of fatty acid derivatives, e.g. dicarboxylic acid (DCA), as polymer building blocks. In contrast to conventional plastics, bioplastics are polymers derived from renewable biomass sources. In addition to their contribution to the conservation of fossil resources and reduction in CO2 emissions by waste incineration, many bioplastics are biodegradable. The majority of industrial DCA production for polyamide (PA) and polyester (PE) synthesis is still done via chemical synthesis. While short-chain DCA can be synthesized in high yields, costs of long-chain DCA production rise significantly due to the generation of various by-products and are connected mostly to a costly purification. Thus biotechnology provides novel biochemical approaches for long-chain DCA synthesis that can provide an eco-efficient process alternative . In the present article, strategies for the development of high-level production strains for long-chain DCA are illustrated. Basic strategies for strain development, in order to achieve an effective enrichment of DCA, require the knowledge of the respective biochemical pathways. These are discussed in detail. Furthermore an overview of fermentation strategies and characteristics of corresponding polymers is given

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Enzym‐Induktion in der Hefe Lodderomyces in Gegenwart von n‐Alkanen

Müller¹,

Mauersberger²,

Schunck³

et al. 1983

J of Basic Microbiology

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The utilization of n-alkanes is connected with extensive modifications of the yeast cell, especially of the cytochrome P-450-containing membrane. Beside the cytochrome P-450 the NADPH cytochrome P-450 reductasa, the cytochrome b6, long-chain alcohol and long-chain aldehyde dehydrogenases are induced. The activity of the alkane-hydroxylating enzyme system grows more than the concentration of its terminal oxidase. The induction of the cytochrome P-460 is inhibited by cyclcheximide. A low concentration of oxygen in the culture medium amplifies the induction both of the alkane-hydroxylating enzyme system and of catalase and cytochrome oxidase, which arelocalized in the peroxisomes and mitochondria, respectively. Von Prokaryoten wie von Eukaryoten ist der

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The induction of cytochrome P-450 inLodderomyces elongisporus

Cited by 28 publications

References 14 publications

Genome‐scale metabolic modeling and in silico analysis of lipid accumulating yeast Candida tropicalis for dicarboxylic acid production

Genome‐scale metabolic modeling and in silico analysis of lipid accumulating yeast Candida tropicalis for dicarboxylic acid production

Biotechnological synthesis of long‐chain dicarboxylic acids as building blocks for polymers

Enzym‐Induktion in der Hefe Lodderomyces in Gegenwart von n‐Alkanen

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