The induction fo collagenase by thyroxine in resorbing tadpole tailfin in vitro

Davis, B.; Jeffrey, John J.; Eisen, Arthur Z.; Derby, A

doi:10.1016/0012-1606(75)90390-5

Cited by 33 publications

(9 citation statements)

References 30 publications

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“…One of the first morphological alterations that occurs is the retraction of the tail fin, a process during which the extracellular matrix is degraded. Associated with this process is the coordinate expression of a neutral collagenase (Davis et al, 1975) and various hydrolytic enzymes (Greenfield and Derby, 1972). At the onset of metamorphic climax, the tail is resorbed and large numbers of phagocytic cells subsequently invade the 2 0 0,O 0 0 -9 7 , 4 0 0 -69,000- connective tissues, ingesting both collagen fibers and dying cells (Yoshizato, 1989).…”

Section: Discussionmentioning

confidence: 99%

A cyclical, developmentally‐regulated death phenomenon in a colonial urochordate

Lauzon

Ishizuka

Weissman

1992

Developmental Dynamics

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Botryllus schlosseri is a colonial ascidian whose asexually derived, clonally modular systems of zooids exhibit developmental synchrony. The blastogenic cycle culminates in a phase of programmed cell and zooid death called takeover, in which all functional zooids die over a 30 hr period, and are replaced by a new generation of individuals. Because of the weekly recurrence and magnitude of visceral death in this model organism, we have begun to characterize the mechanisms that govern takeover. Here we describe a monoclonal antibody (B3F12.9) that recognizes a novel 57 Kd polypeptide (under reducing conditions) localized to the perivisceral extracellular matrix (PVEM) of buds and zooids, as well as blood cells of Botryllus by immunofluorescence and immunogold labeling of tissue sections. During their active feeding phase, zooids exhibited a uniform labeling pattern of PVEM along their anteroposterior (A-P) axis. At the onset of takeover (T = 3 hr), B3F12.9 immunostaining became diffuse or absent at the anterior end, which paralleled the axis of contraction of the dying zooid, whereas the posterior end retained its labeling integrity. During mid (T = 15 hr) to late (T = 28 hr) takeover, issue damage was extensive, large blood macrophages and other B3F12.9 immunoreactive blood cells invaded the peribranchial cavity, whereas PVEM labeling gradually disappeared along the entire A-P axis. These findings indicate that takeover is a dynamic process in which extracellular matrix breakdown proceeds in a polarized fashion, beginning at the anterior end of each zooid and gradually propagating toward the posterior end. o 1992 WiIey-Liss, Inc.

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Section: Discussionmentioning

confidence: 99%

A cyclical, developmentally‐regulated death phenomenon in a colonial urochordate

Lauzon

Ishizuka

Weissman

1992

Developmental Dynamics

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“…De novo protein synthesis is necessary for TH-induced tail resorption (Tata, 1966), suggesting that the complex of TH and thyroid hormone receptor (TR) regulates some genes responsible for cell death in the regressing tail. Based on the observation that TH induces the increase of the collagenase activity in explants of tadpole tailfin and gills and the concomitant decrease of their sizes, it has been proposed that TH-induced collagenase production is involved in the remodeling of collagen in these organs (Davis et al, 1975;Derby et al, 1979). Many genes that are up-and down-regulated by TH in the regressing tail have been isolated by PCR-based subtractive hybridization (Wang and Brown, 1993) and characterized by in situ hybridization (Berry et al, 1998a, b).…”

Section: Introductionmentioning

confidence: 99%

Dual mechanisms governing muscle cell death in tadpole tail during amphibian metamorphosis

Nakajima

Yaoita

2003

Developmental Dynamics

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“…Larval specific organs such as tail and gill are destroyed (9). Tissues of anuran tadpole tail show a TH-mediated regression not only in vivo but also in vitro (5). Several studies have demonstrated that the activity of collagenase increases during TH-induced tail regression of tadpoles of Rana catesbeiana (26) and Xenopus laevis (7).…”

Section: Introductionmentioning

confidence: 99%

Biochemical and Immunological Characterization of Collagenase in Tissues of Metamorphosing Bullfrog Tadpoles

Oofusa

Yoshizato

1991

Dev Growth Differ

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Tissue inhibitor of metalloproteinases (TIMP, a specific inhibitor of collagenase) was found to inhibit thyroid hormone-induced tail regression, suggesting the important role of collagenase in this process. Collagenase was purified from culture media of back skin of tadpole of bullfrog, Rana catesbeiana. Anti-tadpole collagenase polyclonal antisera were obtained against the purified enzyme. The antibody inhibited the activity of tadpole collagenase. The antisera reacted to tissues of adult bullfrogs, tadpoles of african clawed frog, Xenopus laevis, and adult newts, Cynopus pyrrhogaster, and also reacted to human fibroblast collagenase. lmmunoblot analyses suggested that tadpole collagenase lacks the procollagenase which is generally found in mammalian collagenases. Intense immunological stains were observed for the tissues of thyroid hormone-treated tadpoles as compared to those of untreated animals. Thyroid hormone increased amounts of collagenase not only in epidermal layer but also in mesenchymal tissues including fibroblastic cells.

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The induction fo collagenase by thyroxine in resorbing tadpole tailfin in vitro

Cited by 33 publications

References 30 publications

A cyclical, developmentally‐regulated death phenomenon in a colonial urochordate

A cyclical, developmentally‐regulated death phenomenon in a colonial urochordate

Dual mechanisms governing muscle cell death in tadpole tail during amphibian metamorphosis

Biochemical and Immunological Characterization of Collagenase in Tissues of Metamorphosing Bullfrog Tadpoles

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