Biochemical and Immunological Characterization of Collagenase in Tissues of Metamorphosing Bullfrog Tadpoles

Oofusa, Ken; Yoshizato, Katsutoshi

doi:10.1111/j.1440-169x.1991.00329.x

Cited by 27 publications

(19 citation statements)

References 38 publications

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“…The biological significance of the presence of TRE in anuran MMP1 is not clear at present. Our data support the hypothesis that a TRE in anuran MMP1 evolved to regulate the remodeling of larval tissue to adult during metamorphosis (Oofusa & Yoshizato 1991, 1996. Further works are required to determine whether other anuran MMPs contain TRE and if this observation extends to other vertebrates besides humans.…”

supporting

confidence: 84%

“…We prepared specific antisera against Rana MMP1 and cloned its cDNA (Rmmp1) utilizing these antisera. The expression of MMP1 protein and Rmmp1 was greatly enhanced in bullfrog tadpoles during both T 3 -induced and spontaneous metamorphosis (Oofusa & Yoshizato 1991, Oofusa et al 1994. These studies support the notion that MMP1 is responsible for the extensive remodeling of anuran larval tissues taking place during metamorphosis (Yoshizato 1989).…”

Section: Introductionmentioning

confidence: 97%

“…Anuran larvae contain in their life cycle a unique postembryonic developmental process, metamorphosis, in which they extensively remodel their tissues and transform the body structure into the adult one (Yoshizato & Frieden 1975, Niki et al 1984, Yoshizato 1986, 1989, 1992, 1996, 1998, Oofusa & Yoshizato 1991, Shi 2000. The remodeling consists of two processes which proceed concurrently in a tissue, the breakdown of the larval elements, and the genesis and development of the adult elements (Yoshizato 1986, 1989, 1992, Izutsu et al 1993, Kawai et al 1994, Mukai et al 1995.…”

Section: Introductionmentioning

confidence: 99%

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Thyroid hormone response element-like sequence in anuran matrix metalloproteinase 1 gene is responsive to in vivo thyroid hormone administration

Sawada

Oofusa²,

Yoshizato

2001

Journal of Endocrinology

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Gene (Rmmp1) of matrix metalloproteinase 1 (MMP1) of the bullfrog, Rana catesbeiana, has been shown previously to contain a thyroid hormone response element (TRE)-like sequence in its 5 -upstream region. The present study aimed to determine whether this TRE-like sequence is functional in vivo as a true TRE, and to characterize the sequences of the 5 -upstream region with respect to the regulation of the activity of the TRE when the TRE-like sequence was proved to be a true TRE. With this aim, various sequences of TRE-like sequence-containing 5 -upstream region were constructed and fused to the enhanced green fluorescent protein gene as a reporter gene. The fusion constructs were bombarded to the skin of bullfrog tadpoles and the activity of the TRE was quantitatively determined by measuring increased intensities of fluorescence when the animals were exposed to thyroid hormone. The present study clearly demonstrated that the sequence of Rmmp1 is a biologically active TRE in vivo. In addition, a unique 36 bp long sequence directly flanked to the 3 -end of the TRE was identified which worked co-operatively with TRE to regulate the transcriptional promoter activity. It should be emphasized that the presence of TRE in the Rmmp1 gene is unique, because its presence has not been reported in the known promoter region of vertebrate MMPs.

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supporting

confidence: 84%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Thyroid hormone response element-like sequence in anuran matrix metalloproteinase 1 gene is responsive to in vivo thyroid hormone administration

Sawada

Oofusa²,

Yoshizato

2001

Journal of Endocrinology

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“…Using immunohistochemistry, Oofusa and Yoshizato (1991) found that bullfrog MMP1 protein increased in back skin in response to T 3 treatment. These results are interesting because skin from the tadpole trunk undergoes remodeling that is histologically similar to the remodeling observed in the current study (reviewed by Yoshizato, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…MMP1 is a gene that is known to be directly targeted by TH in Rana catesbeiana (Oofusa and Yoshizato, 1996; Sawada et al, 2001). In R. catesbeiana, MMP1 is involved in collagen turnover that occurs during tail resorption and skin remodeling (Oofusa and Yoshizato, 1991). The functions of the rest of these genes (KRT6A, KRT14, GLUL, EMP1, CALM2) during amphibian metamorphosis are largely unknown.…”

Section: Methodsmentioning

confidence: 99%

A model of transcriptional and morphological changes during thyroid hormone-induced metamorphosis of the axolotl

Page

Monaghan

Walker

et al. 2009

General and Comparative Endocrinology

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Anuran (frog) metamorphosis has long-served as a model of how thyroid hormones regulate postembryonic development in vertebrates. However, comparatively little is known about urodele (salamander) metamorphosis. We conducted a detailed time-course study of induced metamorphosis in the Mexican axolotl (Ambystoma mexicanum) that probed metamorphic changes in morphology and gene expression in the skin. Using morphometrics, quantitative PCR, histology, and in situ hybridization we demonstrate that the development of transcriptional markers is fundamental to the resolution of early metamorphic events in axolotls. We then use linear and piecewise linear models to identify a sequence of morphological and transcriptional changes that define larval to adult remodeling events throughout metamorphosis. In addition, we show that transcriptional biomarkers are expressed in specific larval and adult cell populations of the skin and that temporal changes in these biomarkers correlate with tissue remodeling. We compare our results with other studies of natural and induced metamorphosis in urodeles and highlight what appear to be conserved features between urodele and anuran metamorphosis.

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Regionally regulated conversion of protein expression in the skin during anuran metamorphosis

Kobayashi¹,

Satō²,

Yoshizato

1996

J. Exp. Zool.

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The back skin of anuran larvae undergoes drastic metamorphic changes which are quite different from the tail skin. To have an insight into the mechanism of the region‐dependent changes, we made a thorough survey of metamorphic alterations in protein expression in both the body and tail skin of Xenopus laevis tadpoles utilizing high‐resolution two‐dimensional electrophoresis. Approximately 400 proteins were detected, 9 of which were identified as known proteins and were used as index proteins to standardize the readout of distribution of protein spots on gels. The overall pattern of polypeptide distribution (map) was classified into “larval type” and “adult type.” The maps showed stage‐specific changes. Up to stage 58 the back skin map remained the larval type, showed remarkable changes for the first time at stage 60 (whose pattern was a combination of both larval and adult types), and became the adult type at stage 62. The basic feature of the tail skin map remained the larval type through the climax stage of metamorphosis. Experiments using tadpoles treated with an antithyroid drug showed that most of back skin proteins detected on the map are thyroid hormone responsive in their expression. Expression of keratin II (adult‐type keratin) was found to be uniquely regulated. As expected, silver staining detected it in the back but not in the tail. However, the protein‐labeling experiment with [35S]methionine showed that the tail skin is actively synthesizing the adult keratin, suggesting that tail cells have a specific elimination mechanism of the protein. © 1996 Wiley‐Liss, Inc.

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Biochemical and Immunological Characterization of Collagenase in Tissues of Metamorphosing Bullfrog Tadpoles

Cited by 27 publications

References 38 publications

Thyroid hormone response element-like sequence in anuran matrix metalloproteinase 1 gene is responsive to in vivo thyroid hormone administration

Thyroid hormone response element-like sequence in anuran matrix metalloproteinase 1 gene is responsive to in vivo thyroid hormone administration

A model of transcriptional and morphological changes during thyroid hormone-induced metamorphosis of the axolotl

Regionally regulated conversion of protein expression in the skin during anuran metamorphosis

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