1985
DOI: 10.4049/jimmunol.135.1.185
|View full text |Cite
|
Sign up to set email alerts
|

The induction and suppression of in vitro IgG anti-tetanus toxoid antibody synthesis by human lymphocytes stimulated with tetanus toxoid in the absence of in vivo booster immunizations.

Abstract: This report presents a new approach that by-passes booster immunizations with tetanus toxoid (TT) before in vitro studies of antibody (Ab) production. The methodology for optimal TT-induced synthesis of specific IgG anti-tetanus toxoid Ab (IgG anti-TT) by peripheral blood mononuclear cells (PBMC) from randomly selected TT immune individuals without recent booster immunizations is described. PBMC from most normal immune subjects could be repeatedly induced to produce in vitro IgG anti-TT; PBMC from subjects wit… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2

Citation Types

0
0
0

Year Published

1986
1986
2023
2023

Publication Types

Select...
5
1

Relationship

0
6

Authors

Journals

citations
Cited by 20 publications
(2 citation statements)
references
References 0 publications
0
0
0
Order By: Relevance
“…In Vitro Experimental Data Supporting the Model. The in vitro model was used as a guide for establishing the range of immune response variables [18,60,61]. Individual I g G-secreting B-cells were measured by B-cell ELISpots in cultures of PBMC, CD4 + Tcells + non-T-cells (B-cells, monocytes, and NK cells), or CD8 + T-cells + non-T-cells in co-cultures stimulated with PWM (Figure 2B).…”
Section: Overview Of the Mathematical Model A Theoretical Model Was D...mentioning
confidence: 99%
See 1 more Smart Citation
“…In Vitro Experimental Data Supporting the Model. The in vitro model was used as a guide for establishing the range of immune response variables [18,60,61]. Individual I g G-secreting B-cells were measured by B-cell ELISpots in cultures of PBMC, CD4 + Tcells + non-T-cells (B-cells, monocytes, and NK cells), or CD8 + T-cells + non-T-cells in co-cultures stimulated with PWM (Figure 2B).…”
Section: Overview Of the Mathematical Model A Theoretical Model Was D...mentioning
confidence: 99%
“…Individual I g G-secreting B-cells were measured by B-cell ELISpots in cultures of PBMC, CD4 + Tcells + non-T-cells (B-cells, monocytes, and NK cells), or CD8 + T-cells + non-T-cells in co-cultures stimulated with PWM (Figure 2B). The data from individuals published by Lum et al [18,60,61] were reanalyzed and presented in the context of this study. Similarly, purified CD4 + or CD8 + T-cell subsets were evaluated for enhancement or suppression of control culture containing a fixed number of T + B-cells (Figure 2C).…”
Section: Overview Of the Mathematical Model A Theoretical Model Was D...mentioning
confidence: 99%