The index herd with PMWS in Sweden: Presence of serum amyloid A, circovirus 2 viral load and antibody levels in healthy and PMWS-affected pigs

Wallgren, Per; Brunborg, Inger Marit; Blomqvist, Gunilla; Bergström, Günnar; Wikström, Frida Hasslung; Allan, Gordon; Fossum, Caroline; Jonassen, Christine Monceyron

doi:10.1186/1751-0147-51-13

Cited by 15 publications

(7 citation statements)

References 36 publications

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“…All serum samples with a high-level viremia were diagnosed as PCV3 positive by our ELISA; however, in the sera with low-level viremia, 7 of 15 were identified as PCV3 positive by the ELISA. In previous reports, PCV3 titer or genomic copy number from clinical serum samples varied from 10 2 to 10 4 [2], much lower than that of PCV2 (> 10 7 ) [30–32]. Low-level viremia indicates a lower virus titer in serum.…”

Section: Discussionmentioning

confidence: 98%

Development and application of a baculovirus-expressed capsid protein-based indirect ELISA for detection of porcine circovirus 3 IgG antibodies

et al. 2019

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Background Porcine circovirus type 3 (PCV3), recently widely isolated from pigs with various clinical conditions, is likely globally epidemic. However, development of serological diagnosis for PCV3 in pigs is ongoing. Our objectives were to: 1) establish an indirect ELISA, using PCV3 capsid protein (Cap) prepared by Baculovirus Expression Vector System (BEVS) as a high-quality coating antigen for detection of PCV3-associated antibodies in serum samples; and 2) use this ELISA to conduct a serological survey for PCV3 in various regions of Hunan province, China. Results The PCV3 positive rate to the ELISA assay (total of 190 serum samples) was higher in sows with reproductive failure compared to healthy sows (34/85, 40.0% versus 30/105, 28.6%), with similar results using qPCR assays. Further, in an additional 1038 serum samples collected from January 2016 to May 2018 in various regions of Hunan province and tested with this established ELISA, 20 to 84% were positive for PCV3 (according to region of sera collection), with high PCV3 seroprevalence (> 50%) in herds in Changde, Hengyang and Yueyang. Moreover, among serum samples from herds in Shaoyang and Changde, PCV3 seroprevalence was higher in sows than in other classes of pigs (i.e., suckling piglets, nursery pigs, gilts, growing-finishing pigs and boars). Conclusions We developed a full-length PCV3 Cap-based ELISA using a eukaryotic expression system with excellent potential to elucidate PCV3 epidemiology. Based on this assay, PCV3 has been circulating in Hunan province. PCV3 prevalence was lower in healthy sows than in those with reproductive failure. Further studies are warranted to identify the PCV3 responsible for high seroprevalence in sows and determine pathogenesis of PCV3 in sows with reproductive failure. Electronic supplementary material The online version of this article (10.1186/s12917-019-1810-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 98%

Development and application of a baculovirus-expressed capsid protein-based indirect ELISA for detection of porcine circovirus 3 IgG antibodies

et al. 2019

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“…In a study in farms with animals clinically affected by Postweaning Multisystemic Wasting Syndrome (PMWS) caused by PCV2, the serum levels of Hp and Pig-MAP correlate with PCV2 viremia and the clinical course of the disease, concluding that Pig-MAP, in the conditions of this study, was better indicator of the PMWS status than Hp (Grau-Roma et al, 2009). In another study in farms affected by PMWS the increase in the viral load did not induce any SAA response (Wallgren et al, 2009). In pigs experimentally infected by Streptococcus suis showed increased serum concentrations of Hp, CRP, SAA and Pig-MAP (Sorensen et al, 2006).…”

Section: Disease and Apps In Swinementioning

confidence: 51%

Acute Phase Proteins as Biomarkers in Animal Health and Welfare

Gómez-Laguna¹,

Salguero²,

Pallarés³

et al. 2011

Acute Phase Proteins as Early Non-Specific Biomarkers of Human and Veterinary Diseases

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“…Maternal antibodies against PCV2 have been shown to be present in 3-week-old pigs, which gradually decrease to a very low level in 11-week-old pigs [36]. The low levels of antibodies against PCV2 ensure that the pigs are unable to handle a PCV2 infection, leading them to develop PMWS [37,38]. To create a viable PCV2 vaccine approach, a vaccination method should be designed to induce immunity in piglets prior to the time-point when the weaning maternal immunity makes the piglets susceptible to PCV2 infection.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of single dose of an inactivated porcine circovirus type 2 (PCV2) whole-virus vaccine with oil adjuvant in piglets

Yang

Niu

et al. 2012

Acta Vet Scand

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BackgroundPost-weaning multisystemic wasting syndrome (PMWS) associated with PCV2 is one of the most costly diseases currently faced by the swine industry. The development of effective vaccines against PCV2 infection has been accepted as an important strategy in the prophylaxis of PMWS.MethodsIn the present study, a PK-15 cell-adapted formalin-inactivated prototype vaccine candidate was prepared using a strain of PCV2 from China. Inactivation of the virus was accomplished using a standard formalin inactivation protocol. The protective properties of the inactivated PCV2 vaccine were evaluated in piglets. Ten 28-day-old pigs were randomly assigned to two groups, each with five. Group 1 was vaccinated intramuscularly with the inactivated virus preparation; Group 2 received sterile PBS as a placebo. By 28 days post-vaccination (DPV), Groups 1 and 2 were challenged intranasally and intramuscularly with 5 × 107 TCID50 of a virulent PCV2 isolate.ResultsThe vaccinated pigs seroconverted to PCV2 and had high levels of serum antibodies to PCV2 at 28 days after vaccination, whereas the control pigs remained seronegative. No significant signs of clinical disease were recorded following the challenge with PCV2, but moderate amounts of PCV2 antigen were detected in most lymphoid organs of the control pigs. PCV2 was detected in two out of the five vaccinated pigs. Furthermore, pathological lesions and viremia were milder in the vaccinated group.ConclusionsThe obtained results indicate that the inactivated PCV2 virus vaccine with an oil adjuvant induce an immunological response in pigs that appears to provide protection from infection with PCV2. The vaccine, therefore, may have the potential to serve as a vaccine aimed to protect pigs from developing PMWS.

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The index herd with PMWS in Sweden: Presence of serum amyloid A, circovirus 2 viral load and antibody levels in healthy and PMWS-affected pigs

Cited by 15 publications

References 36 publications

Development and application of a baculovirus-expressed capsid protein-based indirect ELISA for detection of porcine circovirus 3 IgG antibodies

Development and application of a baculovirus-expressed capsid protein-based indirect ELISA for detection of porcine circovirus 3 IgG antibodies

Acute Phase Proteins as Biomarkers in Animal Health and Welfare

Efficacy of single dose of an inactivated porcine circovirus type 2 (PCV2) whole-virus vaccine with oil adjuvant in piglets

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