The incorporation of epithelial cells by cementum

Lester, Keith S.

doi:10.1016/s0022-5320(69)90020-3

Cited by 82 publications

(46 citation statements)

References 33 publications

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“…These compartments have been demonstrated by Lester (1969). The fibril bundles were finally released from the compartments, and at that time fibers with different orientation were deposited around the fibril bundles.…”

Section: Discussionmentioning

confidence: 90%

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Comparative study of the initial genesis of acellular and cellular cementum in rat molars

et al. 1994

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The initial genesis of acellular and cellular cementum was examined in rat molars by light and electron microscopy. Before root dentinogenesis, flattened dental follicle cells formed compartments by regularly arranged cellular processes which demarcated collagen fibril bundles oriented in parallel with the root long axis in both of the two kinds of cementum. After this stage, compartments disappeared from the dental follicle cells, which became elongated and polarized, with the cytoplasmic side facing toward the root surface in the acellular cementogenesis. Fibril bundles, oriented in parallel with the root long axis, decreased in number, and principal fibers appeared. Some principal fibers were attached on the first acellular cementum. Observations suggested that the fibril bundles, which had been oriented in parallel with the root long axis, were reoriented to merge into the principal fibers. In cellular cementogenesis, the dental follicle cells continued to hold the fibril bundles in cellular compartments. The regular processes were transformed into randomly oriented, finger-like processes. At the same time, fibers, which may be secreted from the finger-like processes, appeared around the preformed fibril bundles oriented in parallel with the root long axis. The different cellular behavior may result in the different fiber arrangement of acellular and cellular cementum.

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Section: Discussionmentioning

confidence: 90%

“…The intercellular spaces were almost completely closed (Figs. 10b,11), and the cells closest to the root surface are regarded as cementoblasts (Lester 1969;Yamamoto and Hinrichsen 1993). Crosscut fibril bundles were free of cellular compartments on the root surface in transverse sections.…”

Section: Cellular Cementogenesismentioning

confidence: 99%

Comparative study of the initial genesis of acellular and cellular cementum in rat molars

et al. 1994

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“…Cementogenesis starts when the dental sac cells attach to epithelial cells, which may contribute to the formation of either the acellular or the cellular cementum. Lester (1969) and Lindskog (1982) reported that the innermost layer attached to the root dentin is probably formed by inner enamel epithelial cells of the HERS, and not by cementoblasts. Some researchers have reported that cementoblasts are derived from cells of the enamel epithelium which can be transformed into mesenchymal cells (Cho and Garant 1988;Bosshardt and Schroeder 1996).…”

Section: Discussionmentioning

confidence: 99%

Behavior of epithelial root sheath during tooth root formation in porcine molars: TUNEL, TEM, and immunohistochemical studies

Suzuki

Inoue

Shimono

et al. 2002

Anatomy and Embryology

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The behavior of epithelial cells during tooth root formation in porcine molars was investigated using TUNEL, immunohistochemical and transmission electron microscopic methods. Cells of Hertwig's epithelial root sheath (HERS) expressed bcl-2, but were TUNEL-negative. Epithelial cells of HERS were tightly connected and surrounded by a continuous basement membrane. When dental papilla cells were attached to the inner HERS, they became positive for osteopontin (OPN). The inner basement membrane was intermittent immediately after those cells started to synthesize dentin matrix, and the intercellular spaces of the HERS became wider. Dental sac cells then migrated between the fragmented HERS and were directly connected to epithelial cells by desmosome-like structures. After the cementum was synthesized by these mesenchymal cells, epithelial cells aggregated to form cell clusters, which were again surrounded by a continuous basement membrane. These results suggest that fragmentation of the HERS is due to the invasion of dental sac cells, but is not related to cell death. Furthermore, the differentiation of dental papilla cells into odontoblasts occurred following their connection to the HERS through the basement membrane. Moreover, it is suggested that the differentiation of dental sac cells into cementoblasts occurred upon their direct connection to epithelial cells of the HERS.

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“…However, in Hertwig's epithelial root sheath during root formation, and in the lingual surface of rodent incisors, the inner enamel epithelium fails to undergo cytodifferentiation into secretory ameloblasts despite differentiation of mesenchymal cells into odontoblasts and the consequent dentin mineralization. Therefore, for a deeper understanding of epitheliomesenchymal interactions, light and electron microscopy, immunohistochemistry or enzyme histochemistry, and autoradiography have been used to compare these regions with areas showing normal amelogenesis (Lester, 1969;Ten Cate, 1978;Owens, 1980;Lindskog and Hammarstrom, 1982;Nakamura, 1984;Andujar et al, 1985;Suzuki, 1985;Glazman et al, 1986;Nakamura et al, 1986Nakamura et al, , 1987). …”

Section: Introductionmentioning

confidence: 99%

Epithelial cytodifferentiation and extracellular matrix formation in enamel‐free areas of the occlusal cusp during development of mouse molars: Light and electron microscopic studies

1989

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Mandibular first molars in mice ranging in age from 18 days prenatal to 5 days postnatal were used for light and electron microscopic examinations of the enamel-free area (EFA) during development of the occlusal cusp (mesiobuccal cusp). Notable morphological changes in the inner enamel epithelium and the cells of the stratum intermedium were observed. At prenatal age of 18 days, the inner enamel epithelium of the EFA (EFA epithelium) was composed of a layer of columnar cells and covered by the cells of the stratum intermedium. Two days after birth, the EFA epithelium was made up largely of preameloblasts, with mitochondria located in the proximal side of the cells toward the stratum intermedium. The cells of the stratum intermedium were irregularly shaped, with wide intercellular spaces between them. At a postnatal age of 3 days, most of the EFA epithelial cells resembled maturation-stage ameloblasts, being short and columnar in shape and having nuclei located in their proximal side. Distal cell membranes were folded, and mitochondria were scattered throughout the cytoplasm. In 4-day-old mice, the EFA epithelium was found to be formed of short columnar or cuboidal cells with distinct intercellular spaces. The cells of the stratum intermedium could no longer be detected, and cells of the EFA epithelium could not be distinguished from those of the stellate reticulum. Odontoblasts of the EFA were arranged and polarized parallel to the basal lamina, and odontoblastic processes extended toward the cusp tip. The orientation of thin and thick collagen fibers within predentin and dentin was also parallel to the basal lamina. Even after dentin mineralization, disrupted basal lamina and long, aperiodic, fine fibrils were found between the epithelium and the dentin. Following the disappearance of the basal lamina and fine fibrils, stippled material and crystals appeared on the dentin surface. The mineralized matrix, which x-ray microanalytical energy peaks identified as containing calcium and phosphorus, was continuous with enamel in the distal slope of the cusp at the cusp tip. Thus, the inner enamel epithelium of the EFA differentiated into secretory cells capable of enamel-like matrix formation.(ABSTRACT TRUNCATED AT 400 WORDS)

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The incorporation of epithelial cells by cementum

Cited by 82 publications

References 33 publications

Comparative study of the initial genesis of acellular and cellular cementum in rat molars

Comparative study of the initial genesis of acellular and cellular cementum in rat molars

Behavior of epithelial root sheath during tooth root formation in porcine molars: TUNEL, TEM, and immunohistochemical studies

Epithelial cytodifferentiation and extracellular matrix formation in enamel‐free areas of the occlusal cusp during development of mouse molars: Light and electron microscopic studies

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