Behavior of epithelial root sheath during tooth root formation in porcine molars: TUNEL, TEM, and immunohistochemical studies

Suzuki, Michiko; Inoue, Takashi; Shimono, Masaki; Yamada, Satoru

doi:10.1007/s00429-002-0276-3

Cited by 17 publications

(17 citation statements)

References 37 publications

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“…Cho and Garant (1988) present transmission electron micrographs in which dental follicle cells appeared to invade the epithelial sheath with their cytoplasmic projections, suggesting that dental follicle cells force their projections into the epithelial sheath to disintegrate it. An electron microscopy study by Suzuki et al (2002) also suggests that invasion of dental follicle cells causes epithelial sheath fragmentation. Contrary to these opinions, we believe that dental follicle cells approach and extend their projections to the root surface only after enzymatic disintegration of the epithelial sheath.…”

Section: Fragmentation Of Hertwig's Epithelial Root Sheathmentioning

confidence: 99%

“…However, apoptotic cell death does not occur or occurs only rarely during epithelial sheath fragmentation (Kaneko et al 1999;Suzuki et al 2002). Nevertheless, the number of epithelial cells decreases immediately after epithelial sheath fragmentation (see Fig.…”

Section: Fate Of Epithelial Sheath Cellsmentioning

confidence: 99%

“…Many reports have emphasized that some epithelial sheath cells transform into cementoblasts through epithelialmesenchymal transition, or EMT (Thomas 1995;Webb et al 1996;Bosshardt andNanci 1998, 2004;Lésot et al 2000;Zeichner-David et al 2003;Bosshardt 2005;Huang et al 2009;Akimoto et al 2011). This novel idea can explain why the number of epithelial cells decreases immediately after epithelial sheath fragmentation, even though only a few epithelial cells die by apoptosis (Kaneko et al 1999;Suzuki et al 2002). Some investigators, however, have disputed EMT and support the original idea of cementogenesis (Diekwisch 2001;Suzuki et al 2002;Yamamoto et al 2007;Yamamoto and Takahashi 2009).…”

Section: Introductionmentioning

confidence: 96%

“…This novel idea can explain why the number of epithelial cells decreases immediately after epithelial sheath fragmentation, even though only a few epithelial cells die by apoptosis (Kaneko et al 1999;Suzuki et al 2002). Some investigators, however, have disputed EMT and support the original idea of cementogenesis (Diekwisch 2001;Suzuki et al 2002;Yamamoto et al 2007;Yamamoto and Takahashi 2009). …”

Section: Introductionmentioning

confidence: 96%

“…Two mechanisms have been proposed for the degradation. First, external mechanical damage or invasion of dental follicle cells into the epithelial sheath causes degradation (Cho and Garant 1988;Suzuki et al 2002). Alternatively, epithelial sheath cells secrete matrix-degrading enzymes that disintegrate the factors (Hirata and Nakamura 2006).…”

Section: Introductionmentioning

confidence: 99%

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Hertwig’s epithelial root sheath cell behavior during initial acellular cementogenesis in rat molars

Yamamoto

Yamada

et al. 2014

Histochem Cell Biol

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This study was designed to examine developing acellular cementum in rat molars by immunohistochemistry, to elucidate (1) how Hertwig's epithelial root sheath disintegrates and (2) whether epithelial sheath cells transform into cementoblasts through epithelial-mesenchymal transition (EMT).Initial acellular cementogenesis was divided into three developmental stages, which can be seen in three different portions of the root: portion 1, where the epithelial sheath is intact; portion 2, where the epithelial sheath becomes fragmented; and portion 3, where acellular cementogenesis begins. Antibodies against three kinds of matrix proteinases, which degrade epithelial sheath-maintaining factors, including basement membrane and desmosomes, were used to investigate proteolytic activity of the epithelial sheath. Tissue non-specific alkaline phosphatase (TNALP) and keratin were used to investigate EMT. Epithelial sheath cells showed immunoreactivity for all three enzymes at fragmentation, which suggests that epithelial sheath disintegration is enzymatically mediated. Dental follicle cells and cementoblasts showed intense immunoreactivity for TNALP, and from portion 1 through to 3, the reaction extended from the alveolar bone-related zone to the root-related zone. Cells possessing keratin/TNALP double immunoreactivity were virtually absent. Keratin-positive epithelial sheath cells showed negligible immunoreactivity for TNALP, and epithelial cells did not appear to migrate to the dental follicle. Together, these findings suggest that a transition phenotype between epithelial cells and cementoblasts does not exist in the developing dental follicle, and hence that epithelial sheath cells do not undergo EMT during initial acellular cementogenesis.In brief, this study supports the notion that cementoblasts derive from the dental follicle. words

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Section: Fragmentation Of Hertwig's Epithelial Root Sheathmentioning

confidence: 99%

Section: Fate Of Epithelial Sheath Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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Hertwig’s epithelial root sheath cell behavior during initial acellular cementogenesis in rat molars

Yamamoto

Yamada

et al. 2014

Histochem Cell Biol

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Bone resorption control of tooth eruption and root morphogenesis: Involvement of the receptor activator of NF‐κB (RANK)

Castañeda

Simon

Jacques

et al. 2010

Journal Cellular Physiology

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Activation of the receptor activator of NF-κB (RANK) is a crucial step in osteoclastogenesis. Loss- and gain-of-function mutations in the Rank gene cause, respectively, osteopetrosis and several forms of extensive osteolysis. Tooth and alveolar bone alterations are associated with these pathologies but remain to be better characterized. The aim of the present study was to establish the tooth and alveolar bone phenotype of a transgenic mouse model of RANK over-expression in osteoclast precursors. Early tooth eruption and accelerated tooth root elongation were observed subsequent to an increase in osteoclast numbers surrounding the tooth. The final root length appeared not to be affected by RANK over-expression, but a significant reduction in root diameter occurred in both control and root-morphogenesis-defective Msx2 null mutant mice. These results indicate that root length is independent of the surrounding bone resorption activity. In contrast, root diameter is sensitive to the activity of alveolar bone osteoclasts. These data suggest that early eruption and thin root are phenotypic features that could be associated with extensive osteolytic pathologies.

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Immunolocalization of proteoglycans and bone-related noncollagenous glycoproteins in developing acellular cementum of rat molars

et al. 2004

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To elucidate the roles of proteoglycans of (PGs), bone sialoprotein (BSP), and osteopontin (OPN) in cementogenesis, their distribution was investigated in developing and established acellular cementum of rat molars by an immunoperoxidase method. To characterize PGs, antibodies against five species of glycosaminoglycans (GAGS), chondroitin-4-sulfate (C4S), chondroitin-6-sulfate (C6S), unsulfated chondroitin (C0S), dermatan sulfate (DS), and keratan sulfate (KS) were used. Routine histological staining was also applied. With onset of dentin mineralization, the initial cementum appeared on the dentin surface as a hematoxylin-stained fibril-poor layer. Subsequently, primitive principal fibers attached to the initial cementum. As the acellular cementum containing extrinsic fibers covered the initial cementum, the intal cementum formed the cemento-dentinal junction. Following immunohistochemistry at the earliest time of cementogenesis, the initial cementum was intensely immunoreactive for C4S, C6S, C0S, BSP, and OPN. After the initial cementum was embedded, neither the cemento-dentinal junction nor the cementum was immunoreactive for any GAG species. However, the cementum was immunoreactive for any GAG species. However, the cementum and cemento-dentinal were consistently immunoreactive for BSP. Although the cemento-dentinal junction was consistently immunoreactive for OPN, the remaining cementum showed no significant immunoreactivity. Thus, initial acellular cementogenesis requires a dense accumulation of PGs, BSP, and OPN, which may be associated with the mineralization process independently of collagen fibrils and initial principal fiber attachment.

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Behavior of epithelial root sheath during tooth root formation in porcine molars: TUNEL, TEM, and immunohistochemical studies

Cited by 17 publications

References 37 publications

Hertwig’s epithelial root sheath cell behavior during initial acellular cementogenesis in rat molars

Hertwig’s epithelial root sheath cell behavior during initial acellular cementogenesis in rat molars

Bone resorption control of tooth eruption and root morphogenesis: Involvement of the receptor activator of NF‐κB (RANK)

Immunolocalization of proteoglycans and bone-related noncollagenous glycoproteins in developing acellular cementum of rat molars

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