The incidence of the vomeronasal organ in 1000 human subjects and its possible clinical significance

García-Velasco, José; Mondragon, Manuel

doi:10.1016/0960-0760(91)90253-2

Cited by 90 publications

(40 citation statements)

References 3 publications

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“…Subsequently, the organs were often considered to be nonexistent or anomalous in adults (Crosby and Humphrey, 1939;Fenu, 1959;Gabrielle, 1967;Levin, 1925) and age-related degeneration of the VNO was described in fetal humans (Humphrey, 1940;Kjaer and Fischer Hansen, 1996;Nakashima et al, 1985). Such reports are at odds with other investigations that described frequent presence among fetal (Ortmann, 1989;Smith et al, 1996Smith et al, , 1997 and adult human samples (Garcia-Velasco and Mondragon, 1991;Johnson et al, 1985;Moran et al, 1991;Stensaas et al, 1991).…”

Section: Introductionmentioning

confidence: 75%

Searching for the vomeronasal organ of adult humans: Preliminary findings on location, structure, and size

Smith

Siegel

Burrows

et al. 1998

Microsc. Res. Tech.

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Section: Introductionmentioning

confidence: 75%

Searching for the vomeronasal organ of adult humans: Preliminary findings on location, structure, and size

Smith

Siegel

Burrows

et al. 1998

Microsc. Res. Tech.

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“…Indications for the occurrence of primate sex pheromones in vaginal discharge already exist (35). Also, the occurrence of the vomeronasal organ in humans (36,37) and its ability to perceive putative pheromones (38) have been demonstrated. The cloned aphrodisin cDNA fragments as well as the gene will now serve as potentially useful tools for the investigation of aphrodisin/ lipocalin-related pheromone systems and their regulation in mammals.…”

Section: Discussionmentioning

confidence: 99%

cDNA sequence and expression pattern of the putative pheromone carrier aphrodisin.

Mägert¹,

Hadrys²,

Cieslak³

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

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The cDNA sequence for aphrodisin, a lipocalin from hamster vaginal discharge which is involved in pheromonal activity, has been determined. Corresponding genomic clones were isolated and the promoter region was identified. Primer extension analysis revealed an adenosine residue as the main transcription initiation site, located 50 bp upstream of the translation start codon ATG, which is surrounded by a typical Kozak sequence. However, data from polymerase chain reaction analysis suggest the existence of at least one alternative transcription initiation site. The aphrodisin cDNA is 732 bp long and codes for the mature 151-aa aphrodisin and an additional N-terminal 16 Aphrodisin, a 17-kDa glycoprotein of the lipocalin family, has been purified from hamster vaginal discharge (9). It consists of 151 aa and contains two disulfide bonds which connect the cysteine residues at positions 38 and 42 and at positions 57 and 149. Its N terminus is blocked by a pyroglutamate residue, indicating the occurrence of a larger primary translational product (10). Acting via the vomeronasal organ (11), aphrodisin elicits copulatory behavior in male hamsters. At this time it is not quite clear whether an additional ligand is required for biological activity but, if this should be the case, it must be bound very tightly to the protein (12). On the other hand, proteolytic or heat degradation of aphrodisincontaining fractions leads to a loss of activity (9). Functions as pheromone-binding proteins have also been discussed for two other lipocalins, the mouse major urinary protein and a-2U globulin (6, 13).Among the known proteins, rat odorant-binding protein (ROBP) shares the highest sequence homology with aphrodisin (40%). It occurs in nasal mucosa of rats and seems to be responsible for the binding of odorant molecules (refs. 14 (model 340; Applied Biosystems). Five micrograms of the total RNA was subjected to reverse transcription using Moloney murine leukemia virus reverse transcriptase and a synthetic oligo(dT) universal primer (UNIP-2; Fig. 1). One-thirtieth of the cDNA mixture was then used for each of the three-step-cycle PCRs (17) in a model 480 thermocycler with Amplitaq DNA polymerase (both from PerkinElmer) and synthetic primers (Fig. 1).Cloning of PCR Fragments. PCR fragments were separated in 3% NuSieve GTG agarose gels (Biozyme, Oldendorf, Germany) and purified with a Qiaex kit (Qiagen, Hilden, Germany). The ends of the PCR fragments were cleaved with Xba I, whose recognition sequence was included in the PCR primers. After the short cleavage products were removed by Abbreviation: ROBP, rat odorant-binding protein. tTo whom reprint requests should be addressed.

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“…Comparing the location of the NPD with the published locations of the putative VNO revealed that four 38,[41][42][43] of the 7 articles located the putative VNO precisely where we have found the NPD fossae (area X in Figure 2). Area C in Figure 2 correctly locates the VNO anterior to the NPD but places it near the nasal floor, whereas area B in Figure 2 vaguely locates it on the septum posterior to the location Potiquet described and where we observed it in cadaver specimens (compare Figure 2 area B with Figures 1 and 4).…”

Section: Commentmentioning

confidence: 97%

Location and Gross Morphology of the Nasopalatine Duct in Human Adults

Jacob¹,

Zelano²,

Güngör³

et al. 2000

Arch Otolaryngol Head Neck Surg

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show abstract

The incidence of the vomeronasal organ in 1000 human subjects and its possible clinical significance

Cited by 90 publications

References 3 publications

Searching for the vomeronasal organ of adult humans: Preliminary findings on location, structure, and size

Searching for the vomeronasal organ of adult humans: Preliminary findings on location, structure, and size

cDNA sequence and expression pattern of the putative pheromone carrier aphrodisin.

Location and Gross Morphology of the Nasopalatine Duct in Human Adults

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