The Incidence of Hepatitis E Virus in Domestic Pigs

Jacková, Anna; Mandelik, Rene; Novotný, Jaroslav; Salamunova, Slavomira; Vilček, Štefan

doi:10.1515/fv-2017-0029

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…Unfortunately, the HEV genotypes could not be identified because the short RT-PCR product (85 bp) was not suitable for genotype analysis. However, the detection of HEV RNA with a high detection rate in monkey faeces, even higher than that reported in pigs, the primary reservoir [ 34 – 36 ], can be used as a preliminary information that warrants a further investigation to conclude that monkey faeces are a source of HEV.…”

Section: Discussionmentioning

confidence: 99%

Mass spectrometry-based identification and whole-genome characterisation of the first pteropine orthoreovirus isolated from monkey faeces in Thailand

et al. 2018

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BackgroundThe pteropine orthoreovirus (PRV) was isolated from monkey (Macaca fascicularis) faecal samples collected from human-inhabited areas in Lopburi Province, Thailand. These samples were initially obtained to survey for the presence of hepatitis E virus (HEV).ResultsTwo virus isolates were retrieved by virus culture of 55 monkey faecal samples. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was successfully used to identify the viruses as the segmented dsRNA orthoreovirus. Phylogenetic analysis of the Lopburi orthoreovirus whole-genomes revealed relationships with the well-characterised PRVs Pulau (segment L1), Cangyuan (segments L2, M3 and S3), Melaka (segments L3 and M2), Kampar (segments M1 and S2) and Sikamat (segments S1 and S4) of Southeast Asia and China with nucleotide sequence identities of 93.5–98.9%. RT-PCR showed that PRV was detected in 10.9% (6/55) and HEV was detected in 25.5% (14/55) of the monkey faecal samples.ConclusionsPRV was isolated from monkey faeces for the first time in Thailand via viral culture and LC-MS/MS. The genetic diversity of the virus genome segments suggested a re-assortment within the PRV species group. The overall findings emphasise that monkey faeces can be sources of zoonotic viruses, including PRV and HEV, and suggest the need for active virus surveillance in areas of human and monkey co-habitation to prevent and control emerging zoonotic diseases in the future.Electronic supplementary materialThe online version of this article (10.1186/s12866-018-1302-9) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Mass spectrometry-based identification and whole-genome characterisation of the first pteropine orthoreovirus isolated from monkey faeces in Thailand

et al. 2018

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“…In Slovakia, infection was detected for the first time in animals only in 2017, when Jackova et al, published on the prevalence of hepatitis E virus in the domestic pigs. RT-PCR analysis of 269 enteric samples revealed that 32 pigs (11.9%) of all age categories were HEV RNA positive [ 27 ]. Moreover, to date only 83 cases of autochthonous disease in humans have been officially reported.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Hepatitis E Virus in Roma Settlements: A Comparison with the General Population in Slovakia

Halánová

Veselíny

Kalinová

et al. 2018

IJERPH

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Hepatitis E infection is one of the most frequent causes of acute hepatitis in the world. Currently five human genotypes with different geographical distributions and distinct epidemiologic patterns are identified. In Slovakia, only rare cases of hepatitis E have been reported in past years. Because the most important risk factors associated with HEV infection include consumption of contaminated pork meat and poor hygienic standards, the aim of the study was to evaluate the prevalence of anti-HEV total antibodies and the main risk factors for HEV in the population living in separated and segregated Roma settlements (n = 195), which represent places with increased risk of infection in Slovakia and to compare it with the prevalence in the general population (n = 69). Of 264 respondents included in the study, 47 (17.8%) showed positivity for anti-HEV antibodies, 42 of whom were Roma (21.5%, n = 195) and 5 (7.2%, n = 69) non-Roma. The population living in Roma settlements lives in poorer conditions and are at higher risk of HEV in comparison to the general population. However, differences in living conditions within the settlements do not contributed to lower risk of HEV antibody prevalence between Roma living in settlements.

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The Incidence of Hepatitis E Virus in Domestic Pigs

Abstract: Hepatitis E virus (HEV) is

Cited by 2 publications

References 24 publications

Mass spectrometry-based identification and whole-genome characterisation of the first pteropine orthoreovirus isolated from monkey faeces in Thailand

Mass spectrometry-based identification and whole-genome characterisation of the first pteropine orthoreovirus isolated from monkey faeces in Thailand

Seroprevalence of Hepatitis E Virus in Roma Settlements: A Comparison with the General Population in Slovakia

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