The Incidence of Crown Rot of Boxed Bananas in Relation to Microbial Populations of the Crown Tissue

Lukezic, F. L.; Kaiser, W. J.; Martínez, María Mercedes

doi:10.1139/b67-041

Cited by 33 publications

(28 citation statements)

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“…Seasonal fluctuations in crown rot severity have already been reported for situations of natural contamination (Griffee, 1971;Lukezic et al, 1967;Shillingford, 1978), but the present investigation is the first report of such fluctuations in the case of artificial contaminations.…”

Section: Variation Of the Susceptibility Of Bananas To Crown Rot Disementioning

confidence: 45%

“…Finlay and Brown (1993) have found C. musae to be 20 times more pathogenic than Fusarium spp. Other authors have highlighted that C. musae can initiate infection from a low inoculum concentration (Finlay and Brown, 1993;Greene and Goos, 1963;Lukezic et al, 1967;Shillingford, 1976) and that other pathogens require much higher inoculum concentration to induce symptoms (Finlay and Brown, 1993;Griffee, 1971;Krauss, 1996;Krauss et al, 1998). Interestingly, the rotting effect induced by C. musae in our untreated control was greater when the pathogen was inoculated alone than in mixture with other fungi at the same final concentration.…”

Section: Resultsmentioning

confidence: 68%

“…Mason Cladosporium sp., Cephalosporium sp., Penicillium sp., and Aspergillus sp. (Anthony et al, 2004;Greene and Goos, 1963;Griffee and Burden, 1976;Johanson and Blasquez, 1992;Lukezic et al, 1967;Marin et al, 1996;Ogundero, 1987;Shillingford, 1976;Wallbridge and Pinegar, 1975;Wallbridge, 1981). Although field infection cannot be excluded, contamination of the wounded crown occurs while hands are being harvested (Meredith, 1971).…”

Section: Introductionmentioning

confidence: 99%

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Biological control of crown rot of bananas with Pichia anomala strain K and Candida oleophila strain O

2008

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The antagonistic activity of two yeast strains (Pichia anomala (E.C. Hansen) Kurtzman, strain K and Candida oleophila Montrocher, strain O) against the parasitic complex responsible for banana crown rot was evaluated. The strains were applied at three different concentrations (10 6 , 10 7 , 10 8 cfu/ml) and their efficacy tested in vivo on three separate fungi (Colletotrichum musae (Berk. & Curt.) Arx, Fusarium moniliforme Sheldon, and Cephalosporium sp.) and on a parasitic complex formed by association of these three fungi. At the concentrations used C. musae appeared to be the most pathogenic. The complex showed intermediate aggressiveness between C. musae and both other fungi.Statistically significant antagonistic effects were observed on C. musae, F. moniliforme, and the fungal complex. The highest protection level (54.4%) was observed with strain O added at 10 8 cfu/ml on crowns previously inoculated with the fungal complex. The level was lower when the fungi were inoculated separately.Furthermore, the antagonistic effect was strongly reinforced when strain O at 10 8 cfu/ml was applied 24 h before fungal complex inoculation (59.9%), as compared to its application 15 min (24.3%) or 3 h (27.3%) after fungal complex inoculation. Bananas showed increased susceptibility to the fungal complex from March to June, and this influenced the level of protection by yeast, which decreased over the same period. A strict negative correlation (R 2 = 0.83) was highlighted between susceptibility of banana to crown rot and protection provided by yeast.

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Section: Variation Of the Susceptibility Of Bananas To Crown Rot Disementioning

confidence: 45%

Section: Resultsmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

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Biological control of crown rot of bananas with Pichia anomala strain K and Candida oleophila strain O

2008

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“…It is caused by a large complex of fungal pathogens varying according to the geographic location and time of year (Meredith, 1971). Among the organisms composing this complex, Colletotrichum musae is often regarded as the most pathogenic (Greene and Goos, 1963;Lukezic et al, 1967;Shillingford, 1976;Finlay and Brown, 1993;Lassois et al, 2008). First symptoms may be observed after shipment, increasing rapidly during fruit ripening (Slabaugh and Grove, 1982).…”

Section: Introductionmentioning

confidence: 99%

Integrated control of crown rot of banana with Candida oleophila strain O, calcium chloride and modified atmosphere packaging

et al. 2010

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a b s t r a c tAn integrated approach for biological control of crown rot of banana was studied. The efficacy of three control measures, applied alone or in various combinations, was evaluated under conditions highly conducive to the development of crown rot (artificial inoculation of Colletotrichum musae at 10 4 conidia/ml).The studied measures were: application of an antagonistic yeast (Candida oleophila strain O at 1.10 7 cfu/ ml), treatment with 2% (w/v) calcium chloride, and modified atmosphere packaging of fruit (MAP) in nonperforated polyethylene bags. C. oleophila was able to grow under MAP, maintaining a large population (7.10 6 to 7.10 7 cfu/g crown) throughout the 13 days of storage. Both treatment with the antagonistic yeast and storage under MAP, applied separately, reduced crown rot significantly (by 22% and 20%, respectively, as compared to untreated controls). The effect of the yeast was the same whether it was produced in Petri dishes or in a fermentor. Calcium chloride treatment alone had no effect on C. musae. The antagonistic yeast showed a 16% higher biocontrol activity (from 26% to 42%) when applied together with 2% (w/v) calcium chloride, and the presence of this adjuvant made it possible to achieve the same protective effect with a lower yeast concentration. The highest efficacy (53%) was achieved by the combination of the three alternatives means of control and a synergistic relation has been detected between the yeast, calcium chloride and MAP. Considering the severe conditions of screening, the consistency of the results obtained in this study indicates that the integrated strategy has great potential for control of crown rot of banana under commercial conditions.

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“…Variations, notably geographic and seasonal, have been reported in the postharvest development of crown rot disease symptoms (Krauss and Johanson 2000;Lassois et al 2008;Lukezic et al 1967;Shillingford 1978). It has been suggested that these spatiotemporal fluctuations reflect the variations in the banana fruit quality potential that elaborates during preharvest stages (Lassois et al 2010b).…”

mentioning

confidence: 99%

Identification of Genes Involved in the Response of Banana to Crown Rot Disease

Lassois¹,

Frettinger²,

Bellaire³

et al. 2011

MPMI

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Variations in banana susceptibility to crown rot disease have been observed but the molecular mechanisms underlying these quantitative host-pathogen relationships are still unknown. This study was designed to compare gene expression between crowns of banana fruit showing a high susceptibility (S + ) and crowns showing a low susceptibility (S -) to the disease. Comparisons were performed at two situation times: i) between crowns (S + and S -) collected 1 h before inoculation and ii) between crowns (S + and S -) collected 13 days after inoculation. Gene expression comparisons were performed with cDNA-amplified fragment length polymorphism (AFLP) and results were confirmed by realtime reverse-transcription polymerase chain reaction. Among genes identified as differentially expressed between S + and S -crowns, two were involved in signal transduction, three in proteolytic machinery, two had similarity to pathogenesis-related protein 14, one to a CCR4-associated factor protein, and one to a cellulose synthase. Paradoxically, the overexpression of the cellulose synthase gene was associated with banana showing a high susceptibility in both preand post-inoculation situations. Finally, the cDNA-AFLP identified a gene that seems to be associated with the quantitative banana responses to crown rot disease; this gene encodes a dopamine-β-monooxygenase, which is involved in the catecholamine pathway. To our knowledge, this work is the first to address both pre-and post-infection gene expression with the same host-pathogen combination and distinct susceptibility levels.

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The Incidence of Crown Rot of Boxed Bananas in Relation to Microbial Populations of the Crown Tissue

Cited by 33 publications

References 1 publication

Biological control of crown rot of bananas with Pichia anomala strain K and Candida oleophila strain O

Biological control of crown rot of bananas with Pichia anomala strain K and Candida oleophila strain O

Integrated control of crown rot of banana with Candida oleophila strain O, calcium chloride and modified atmosphere packaging

Identification of Genes Involved in the Response of Banana to Crown Rot Disease

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