The inactivation of adenoviruses by ultraviolet irradiation and nitrous acid

Wassermann, Felix E.

doi:10.1016/0042-6822(62)90124-1

Cited by 24 publications

(4 citation statements)

References 14 publications

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“…Cameron et al (4) reported increased UV resistance of adenovirus (AD5) compared to other dsDNA viruses (simian virus 40 and herpes simplex virus type 1). In addition, Wasserman (31) reported that AD4 and -20 were more resistant to UV light than AD1, -19, and -24, demonstrating varied susceptibilities between adenovirus types. This was also demonstrated by the research carried out by Cameron (4), where AD5 was more resistant than AD7 to UV light inactivation (4).…”

Section: Resultsmentioning

confidence: 99%

Inactivation of Feline Calicivirus and Adenovirus Type 40 by UV Radiation

Thurston-Enriquez

Haas

Jacangelo³

et al. 2003

Appl Environ Microbiol

238

221

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Little information regarding the effectiveness of UV radiation on the inactivation of caliciviruses and enteric adenoviruses is available. Analysis of human calicivirus resistance to disinfectants is hampered by the lack of animal or cell culture methods that can determine the viruses' infectivity. The inactivation kinetics of enteric adenovirus type 40 (AD40), coliphage MS-2, and feline calicivirus (FCV), closely related to the human caliciviruses based on nucleic acid organization and capsid architecture, were determined after exposure to low-pressure UV radiation in buffered demand-free (BDF) water at room temperature. In addition, UV disinfection experiments were also carried out in treated groundwater with FCV and AD40. AD40 was more resistant than either FCV or coliphage MS-2 in both BDF water and groundwater. The doses of UV required to achieve 99% inactivation of AD40, coliphage MS-2, and FCV in BDF water were 109, 55, and 16 mJ/cm 2 , respectively. The doses of UV required to achieve 99% inactivation of AD40, coliphage MS-2, and FCV in groundwater were slightly lower than those in BDF water. FCV was inactivated by 99% by 13 mJ/cm 2 in treated groundwater. A dose of 103 mJ/cm 2 was required for 99% inactivation of AD40 in treated groundwater. The results of this study indicate that if FCV is an adequate surrogate for human caliciviruses, then their inactivation by UV radiation is similar to those of other single-stranded RNA enteric viruses, such as poliovirus. In addition, AD40 appears to be more resistant to UV disinfection than previously reported.

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Section: Resultsmentioning

confidence: 99%

Inactivation of Feline Calicivirus and Adenovirus Type 40 by UV Radiation

Thurston-Enriquez

Haas

Jacangelo³

et al. 2003

Appl Environ Microbiol

238

221

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“…The end point was considered to be the highest dilution of virus producing cytopathic effect in at least one of two cultures after 7 days of incubation at 36 C. [The technique employed for virus assay yielded a quantitative estimate of virus titer which was approximately 1.0 to 1.5 logio units lower than when cytopathic end point was scored 14 days after infection or 2.0 to 3.0 logio units lower than the virus titer obtained with a dependable plaque assay which was established in our laboratory, after the major portion of this investigation was completed. The plaque assay now employed is a modification of methods reported earlier by Bonifas and Schlesinger (1959), Kjellen (1961), and Wasserman (1962). ]…”

Section: Methodsmentioning

confidence: 99%

Role of Ribonucleic Acid Biosynthesis in Multiplication of Type 5 Adenovirus

Flanagan

Ginsberg

1964

J Bacteriol

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vania, Philadelphia), AND HAROLD S. GINSBERG. Role of ribonucleic acid biosynthesis in multiplication of type 5 adenovirus. J. Bacteriol. 87:977-987. 1964.-The requirement for ribonucleic acid (RNA) biosynthesis in the multiplication of type 5 adenovirus was investigated by using radioactive phosphorus to label nucleic acids and two pyrimidine analogues, 6-azauridine and 5-fluorouracil or 5-fluorouridine, to inhibit synthesis of functional RNA. The data indicate that biosynthesis of RNA after infection is essential for production of virusspecific deoxyribonucleic acid, virus antigens, and infectious particles. The onset of essential RNA synthesis occurs 8 to 9 hr after virus infection and prior to the biosynthesis of other known virusinduced macromolecules.

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“…The data for the inactivation of Ad2 plaque formation on normal human fibroblasts in vitro indicate a UVC exposure of 204.5 mJ/cm 2 for 4‐log inactivation. However, it is not known how applicable this is to other adenoviruses because UVC inactivation of adenovirus is dependent on serotype (Wasserman, 1962).…”

Section: Efficacy Of Drinking Water Treatmentmentioning

confidence: 99%

Effect of adenovirus resistance on UV disinfection requirements: A report on the state of adenovirus science

Yates¹,

Malley²,

Rochelle³

et al. 2006

Journal AWWA

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The dosage of ultraviolet (UV) light required to inactivate adenoviruses serves as the basis for virus inactivation requirements in the Long Term 2 Enhanced Surface Water Treatment Rule.The rule increases the required UV dose from the standard practice of 40 mJ/cm 2 to 186 mJ/cm 2 for 4-log inactivation. Ensuring this delivered dose in the UV reactor requires accounting for uncertainties in the reactor validation testing, which results in an applied UV dose of 200-300 mJ/cm 2 for 4-log virus inactivation credit for a given UV reactor. Concerned about the potential effect of this action on drinking water treatment, a group of experts met to assess the state of the science with respect to adenoviruses in drinking water. This working group reviewed the current science on adenoviruses and identified the effects-positive and negative-on public health protection arising from the elevation of UV design target doses.

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The inactivation of adenoviruses by ultraviolet irradiation and nitrous acid

Cited by 24 publications

References 14 publications

Inactivation of Feline Calicivirus and Adenovirus Type 40 by UV Radiation

Inactivation of Feline Calicivirus and Adenovirus Type 40 by UV Radiation

Role of Ribonucleic Acid Biosynthesis in Multiplication of Type 5 Adenovirus

Effect of adenovirus resistance on UV disinfection requirements: A report on the state of adenovirus science

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