Role of Ribonucleic Acid Biosynthesis in Multiplication of Type 5 Adenovirus

Abstract

vania, Philadelphia), AND HAROLD S. GINSBERG. Role of ribonucleic acid biosynthesis in multiplication of type 5 adenovirus. J. Bacteriol. 87:977-987. 1964.-The requirement for ribonucleic acid (RNA) biosynthesis in the multiplication of type 5 adenovirus was investigated by using radioactive phosphorus to label nucleic acids and two pyrimidine analogues, 6-azauridine and 5-fluorouracil or 5-fluorouridine, to inhibit synthesis of functional RNA. The data indicate that biosynthesis of RNA after infection is essential for production of virusspecific deoxyribonucleic acid, virus antigens, and infectious particles. The onset of essential RNA synthesis occurs 8 to 9 hr after virus infection and prior to the biosynthesis of other known virusinduced macromolecules.

Intracellular Uncoating of Type 5 Adenovirus Deoxyribonucleic Acid

“…Assembly of infectious virus is preceded by the biosynthesis of viral DNA and protein, beginning approximately 10 and 12 hr, respectively, postinfection (6,10,32). The earliest virus-specific biosynthetic event which has been detected is the essential synthesis of RNA at 9 hr after infection (7). This has been demonstrated to be viral messenger RNA (30); (Bello and Ginsberg, in preparation).…”

Activity of Aspartate Transcarbamylase in Uninfected and Type 5 Adenovirus-Infected Hela Cells

“…Relationship of increased A TCase activity and nucleic acid biosynthesis. Adenovirus infection induced increased biosynthesis of RNA between 9 and 14 hr after infection (Flanagan and Ginsberg, 1964), and of DNA between 10 and 24 hr (Ginsberg and Dixon, 1961 1. Aspartate transcarbamylase activity in uninfected, type 5 adenovirus-infected, and toxintreated HeLa cells.…”

“…berg, 1962). Previous studies have shown that the increased synthesis of RNA (Flanagan and Ginsberg, 1964), as well as of DNA, could be inhibited by 5-fluoro-2-deoxyuridine (FUDR; Flanagan and Kjellin, 1962;Green, 1962a). Because ATCase is essential for pyrimidine biosynthesis, experiments were conducted to determine whether FUDR could prevent the increase in ATCase activity in adenovirus-infected cells.…”

“…Infection of mammalian cells with adenovirus in vivo initiates increased biosynthesis of deoxyribonucleic acid (DANA) and ribonucleic acid (RNA; Boyer, Leuchtenberger, and Ginsberg, 1957;Dixon, 1959, 1961; Green, 1959;Green and Daesch, 1961). Not only is DNA biosynthesis essential for replication of the DNA-containing adenovirus Dixon, 1959, 1961; Flanagan and ; Kjellen, 1962;Green, 1962a), but synthesis of RNA similarly is required (Flanagan and Ginsberg, 1964). The DNA produced appears to be the precursor of virus DNA, although only a small portion of the DNA synthesized is actually incorporated into infectious particles Dixon, 1959, 1961).…”

“…The DNA produced appears to be the precursor of virus DNA, although only a small portion of the DNA synthesized is actually incorporated into infectious particles Dixon, 1959, 1961). Although no direct experimental evidence is yet available, the data suggest that the RNA produced after virus infection is messenger RNA and is vital for biosynthesis of the components of the virus particles, structural proteins, and DNA (Flanagan and Ginsberg, 1964). 1 A preliminary report of this work has appeared (Consigli and Ginsberg, 1962).…”