The in vivo effect of the administration of resistance-modulating agents on rhodamine 123 distribution in mice thymus and lymph nodes

Marques‐Santos, Luis Fernando; Harab, Ramza Cabral; Paula, Emílio F.; Rumjanek, Vivian M.

doi:10.1016/s0304-3835(98)00348-6

Cited by 7 publications

(7 citation statements)

References 18 publications

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“…The utilization of ABC transporter protein fluorescent substrates has been extensively used to study the activity of these proteins in a wide range of cell types [25][26][27][28][29]. Using an efflux assay based on intracellular C-AM accumulation, we observed that embryonic cells of E. lucunter accumulated less C-AM than gametes (Figure 1).…”

Section: Discussionmentioning

confidence: 87%

Characterization of functional activity of ABCB1 and ABCC1 proteins in eggs and embryonic cells of the sea urchin Echinometra lucunter

et al. 2010

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ABC transporter (ATP-binding-cassette transporter) proteins have been strongly associated with the phenomenon of multidrug resistance in cancer cells. Furthermore, their physiological expression has been studied in many organisms, including bacteria, fungi, plants and vertebrate or invertebrate animals. Their widespread expression through the evolution demonstrates their relevance to the survival of living things. In the present study, we characterized the functional activity of ABCB1 and ABCC1 proteins in gametes and embryonic cells of the sea urchin Echinometra lucunter. The ABC transporter proteins' functional activity was up-regulated post-fertilization. Eggs and spermatozoa of E. lucunter accumulated more C-AM (calcein acetoxymethyl ester), a fluorescent substrate of ABCB1 and ABCC1 proteins, than embryonic cells. Verapamil, reversin 205 and indomethacin were able to increase C-AM influx in eggs and embryos. However, verapamil and reversin 205 were more efficient than indomethacin, suggesting a predominance of ABCB1 protein over ABCC1 protein activity. Multidrug resistance modulating agents, at the concentration range that inhibited ABC transporter proteins, did not block the embryonic development until blastula stage. However, inhibition of ABCB1-mediated efflux by reversin 205 circumvented resistance of embryos to the antimitotic vinca alkaloid vinblastine. Embryonic development was more efficiently blocked when colchicine was previously added to eggs than to embryos 5 min after fertilization. This set of results suggests that these proteins act as a fundamental biochemical barrier conferring a protective physiological role against toxic xenobiotics in E. lucunter embryos.

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Section: Discussionmentioning

confidence: 87%

Characterization of functional activity of ABCB1 and ABCC1 proteins in eggs and embryonic cells of the sea urchin Echinometra lucunter

et al. 2010

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“…Recently, the functional study of P-gp was of great concern. Among lymphocytes in the mouse, CD8 + cells expressed the highest levels of P-gp followed by CD4 + > CD3 + [6, 21, 29–32]. Since blood samples are easy to be collected, we selected peripheral blood CD8 + cells as a surrogate indicator to investigate the alteration of P-gp activities before and after the administration of P-gp inhibitors and then evaluate the reversal effect of the drug in a solid-tumor-bearing mouse.…”

Section: Discussionmentioning

confidence: 99%

“…To study the effects of Rho123 administration on peripheral blood CD8 + cells, the retention of fluorescence in these cells was investigated as described previously [21] with some modifications and a dose-response curve established. On the 8th day after the Hca/FAP injection, the mice received a single intravenous (i.v.)…”

Section: Methodsmentioning

confidence: 99%

Using Rhodamine 123 Accumulation in CD8⁺ Cells as a Surrogate Indicator to Study the P‐Glycoprotein Modulating Effect of Cepharanthine Hydrochloride In Vivo

Han

Zhang

Wang

et al. 2011

BioMed Research International

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The purpose of this study was the use of rhodamine 123 (Rho123) accumulation in peripheral blood CD8+cells as a surrogate indicator to evaluate the modulating effect of P-glycoprotein (P-gp) inhibitors in the multidrug resistance (MDR) tumor-bearing mouse model. Rho123 was administered to mice, and the fluorescence level in CD8+ cells was measured. Cepharanthine hydrochloride (CH) and verapamil (VER), two P-gp inhibitors, were administered to mice 1 hour prior to Rho123 administration in vivo or added to peripheral blood 1 hour prior to Rho123 addition ex vivo. The tumor inhibition effect of 5-fluorouracil/adriamycin/cisplatin (FAP) protocol plus CH was also investigated. A concentration- or dose-response relationship was shown between the concentration and dose of CH and Rho123 accumulation or the antitumor activity. In conclusion, the measurement of Rho123 accumulation in CD8+ cells provides a surrogate assay for the screening of candidate P-gp inhibitors in preclinical trials, and CH is effective in modulating P-gp-mediated MDR in vivo.

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“…To study ABCB1 activity in vivo Rho 123 was injected in association or not with cyclosporine A as described before [35] with small modifications. Briefly, 2-4 months old mice received a single intraperitoneal (i.p.)…”

Section: Abcb1 and Abcc1 Activity In Vivomentioning

confidence: 99%

In vivo and in vitro modulation of MDR molecules in murine thymocytes

Leite

Echevarria-Lima

Salgado

et al. 2006

International Immunopharmacology

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The in vivo effect of the administration of resistance-modulating agents on rhodamine 123 distribution in mice thymus and lymph nodes

Cited by 7 publications

References 18 publications

Characterization of functional activity of ABCB1 and ABCC1 proteins in eggs and embryonic cells of the sea urchin Echinometra lucunter

Characterization of functional activity of ABCB1 and ABCC1 proteins in eggs and embryonic cells of the sea urchin Echinometra lucunter

Using Rhodamine 123 Accumulation in CD8⁺ Cells as a Surrogate Indicator to Study the P‐Glycoprotein Modulating Effect of Cepharanthine Hydrochloride In Vivo

In vivo and in vitro modulation of MDR molecules in murine thymocytes

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The in vivo effect of the administration of resistance-modulating agents on rhodamine 123 distribution in mice thymus and lymph nodes

Cited by 7 publications

References 18 publications

Characterization of functional activity of ABCB1 and ABCC1 proteins in eggs and embryonic cells of the sea urchin Echinometra lucunter

Characterization of functional activity of ABCB1 and ABCC1 proteins in eggs and embryonic cells of the sea urchin Echinometra lucunter

Using Rhodamine 123 Accumulation in CD8+ Cells as a Surrogate Indicator to Study the P‐Glycoprotein Modulating Effect of Cepharanthine Hydrochloride In Vivo

In vivo and in vitro modulation of MDR molecules in murine thymocytes

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Using Rhodamine 123 Accumulation in CD8⁺ Cells as a Surrogate Indicator to Study the P‐Glycoprotein Modulating Effect of Cepharanthine Hydrochloride In Vivo