The in vivo antineoplastic and therapeutic efficacy of troxerutin on rat preneoplastic liver: biochemical, histological and cellular aspects

Thomas, Nisha Susan; George, Kiran; Arivalagan, S.; Mani, Vijay; Siddique, Aktarul Islam; Nalini, Namasivayam

doi:10.1007/s00394-016-1275-0

Cited by 25 publications

(18 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown by our study, TRX treatment (100 mg/kg) reverses the 5-FU-induced increase of MDA levels and prevents GSH consumption in mice. The antioxidant effect of TRX shown in the present study has also been reported by several published studies in different experimental models [17][18][19]21,[57][58][59][60][61].…”

Section: Discussionsupporting

confidence: 90%

“…The body weight of mice was assessed daily prior to administration of the administered treatment to confirm the experimental model of 5-FU induced intestinal mucositis. The TRX doses considered in the current study are in accordance with those in previous published studies [19][20][21][22]61]. daily prior to administration of the administered treatment to confirm the experimental model of 5-FU induced intestinal mucositis.…”

Section: Experimental Protocol Of 5-fu-induced Intestinal Mucositissupporting

confidence: 70%

“…daily prior to administration of the administered treatment to confirm the experimental model of 5-FU induced intestinal mucositis. The TRX doses considered in the current study are in accordance with those in previous published studies [19][20][21][22]61]. To investigate the role of cyclooxygenase 2 (COX-2) enzyme during TRX treatment of 5-FUinduced intestinal mucositis, COX-2 was blocked by an intraperitoneal (i.p) injection of 7.5 mg/kg of CLX.…”

Section: Experimental Protocol Of 5-fu-induced Intestinal Mucositismentioning

confidence: 85%

See 2 more Smart Citations

Troxerutin Prevents 5-Fluorouracil Induced Morphological Changes in the Intestinal Mucosa: Role of Cyclooxygenase-2 Pathway

et al. 2020

View full text Add to dashboard Cite

Intestinal mucositis is a common complication associated with 5-fluorouracil (5-FU), a chemotherapeutic agent used for cancer treatment. Troxerutin (TRX), a semi-synthetic flavonoid extracted from Dimorphandra gardneriana, has been reported as a potent antioxidant and anti-inflammatory agent. In the present study, we aimed to evaluate the effect of TRX on 5-FU-induced intestinal mucositis. Swiss mice were randomly divided into seven groups: Saline, 5-FU, TRX-50, TRX-100, TRX-150, Celecoxib (CLX), and CLX + TRX-100. The weight of mice was measured daily. After treatment, the animals were euthanized and segments of the small intestine were collected to evaluate histopathological alterations (morphometric analysis), levels of malondialdehyde (MDA), myeloperoxidase (MPO), glutathione (GSH), mast and goblet cell counts, immunohistochemical analysis, and cyclooxygenase-2 (COX-2) activity. Compared to the saline treatment, the 5-FU treatment induced intense weight loss and reduction in villus height. TRX treatment (100 mg/kg) prevented the 5-FU-induced histopathological changes and decreased oxidative stress by decreasing the MDA levels and increasing GSH concentration. TRX attenuated inflammatory process by decreasing MPO activity, intestinal mastocytosis, and COX-2 expression. TRX also reversed the depletion of goblet cells. Our findings suggest that TRX at a concentration of 100 mg/kg had chemopreventive effects on 5-FU-induced intestinal mucositis via COX-2 pathway.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Experimental Protocol Of 5-fu-induced Intestinal Mucositissupporting

confidence: 70%

Section: Experimental Protocol Of 5-fu-induced Intestinal Mucositismentioning

confidence: 85%

See 1 more Smart Citation

Troxerutin Prevents 5-Fluorouracil Induced Morphological Changes in the Intestinal Mucosa: Role of Cyclooxygenase-2 Pathway

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Troxerutin has been shown to reverse body weight (79), lipid accumulation (40), and fat-induced fibrotic changes (39), reduce xenobiotic enzymes, and prevent D-galactoseinduced inflammation and oxidation (80,81), whereas its effect on lipotoxicity is largely unknown. Acaa2 and Acox1 were highly expressed in renal tubular epithelial cells of troxerutin treatment, verifying that fatty acid oxidation was provoked after remission of lipid accumulation.…”

Section: Discussionmentioning

confidence: 99%

Precision toxicology shows that troxerutin alleviates ochratoxin A–induced renal lipotoxicity

Yang

Huang

et al. 2018

FASEB j.

View full text Add to dashboard Cite

Lipotoxicity is the most common cause of severe kidney disease, with few treatment options available today. Precision toxicology can improve detection of subtle intracellular changes in response to exogenous substrates; thus, it facilitates in‐depth research on bioactive molecules that may interfere with the onset of certain diseases. In the current study, troxerutin significantly relieved nephrotoxicity, increased endurance, and improved systemic energy metabolism and renal inflammation in OTA‐induced nephrotic mice. Lipidomics showed that troxerutin effectively reduced the levels of triglycerides, phosphatidylcholines, and phosphatidylethanolamines in nephropathy. The mechanism was partly attributable to troxerutin in alleviating the aberrantly up‐regulated expression of sphingomyelinase, the cystic fibrosis transmembrane conductance regulator, and chloride channel 2. Renal tubular epithelial cells, the main site of toxin‐induced accumulation of lipids in the kidney, were subjected to transcriptomic profiling, which uncovered several metabolic factors relevant to aberrant lipid and lipoprotein metabolism. Our work provides new insights into the molecular features of toxin‐induced lipotoxicity in renal tubular epithelial cells in vivo and demonstrates the function of troxerutin in alleviating OTA‐induced nephrosis and associated systemic energy metabolism disorders.—Yang, X., Xu, W., Huang, K., Zhang, B., Wang, H., Zhang, X., Gong, L., Luo, Y., He, X. Precision toxicology shows that troxerutin alleviates ochratoxin A‐induced renal lipotoxicity. FASEB J. 33, 2212–2227 (2019). http://www.fasebj.org

show abstract

“…The other two flavonoids rutin and troxerutin exerted anticancer properties in several cancer cell lines through modulating multiple signaling pathways. [17] However, in prostate cancer cells rutin exhibited very low level of cell proliferation inhibition. [18] Our present study results reveal that rutin and troxerutin did not cause any changes in the I K current properties in human prostate LNCaP cells.…”

Section: Discussionmentioning

confidence: 99%

Untitled

Malathi

2017

AJP

View full text Add to dashboard Cite

Background: Phenolic compounds are ubiquitous in dietary fruits and vegetables and have been proposed to have antioxidant, antibacterial, anti-inflammatory, and anticancer effects. Voltage gated K + channels (I K ) contribute to various cellular mechanisms involved in the tumor cell progression. Therefore, the modulation of I K current may be a potential target for cancer therapy. In this study, we electrophysiologically characterized four phenolic compounds for their modulatory activities on I K currents. Materials and Methods: The whole cell configuration of the patch clamp recording technique was used to record the I K currents from human prostate cancer line (LNCaP). In addition, 200 µM of phenolic compounds (curcumin, resveratrol, rutin, and troxerutin) were externally perfused to the cells. Results: Obtained results provide evidence that resveratrol at 200 µM inhibited more than half of the I K current amplitude. On the other hand, curcumin, rutin, and troxerutin did not show any modulatory effect on I K current in LNCaP cells. Conclusion: This study demonstrates that not all the anticancer compounds are effective inhibitors of I K current, but only few of them. The I K current inhibitors might exhibit effective antimetastatic properties.

show abstract

The in vivo antineoplastic and therapeutic efficacy of troxerutin on rat preneoplastic liver: biochemical, histological and cellular aspects

Abstract: This study provides the evidence that troxerutin exerts a significant therapeutic effect against liver cancer by modulating liver function enzymes, xenobiotic enzymes, oxidative damage, inhibiting cell proliferation, suppressing inflammatory response and induction of apoptosis.

Cited by 25 publications

References 53 publications

Troxerutin Prevents 5-Fluorouracil Induced Morphological Changes in the Intestinal Mucosa: Role of Cyclooxygenase-2 Pathway

Troxerutin Prevents 5-Fluorouracil Induced Morphological Changes in the Intestinal Mucosa: Role of Cyclooxygenase-2 Pathway

Precision toxicology shows that troxerutin alleviates ochratoxin A–induced renal lipotoxicity

Untitled

Contact Info

Product

Resources

About