The In Vitro Inhibition of Human Neutrophil Elastase Activity by some Yemeni Medicinal Plants

Alasbahi, Rawiya H.

doi:10.3797/scipharm.0804-25

Cited by 18 publications

(8 citation statements)

References 33 publications

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“…It will play a destructive role as a deficiency of the endogenous inhibitor of HNE [10]. Insufficient levels of protease inhibitors have been suggested as a contributing factor in a number of diseases including acute lung injury, cystic fibrosis, ischemic reperfusion injury, rheumatoid arthritis, atherosclerosis, psoriasis, and malignant tumors [11]. Sivelestat, which is currently used as a synthetic elastase inhibitor, is applied for the treatment of acute lung injury and acute respiratory distress syndrome (ARDS).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MSn and Their Inhibitory Effect on Human Neutrophil Elastase Activity

Huang

Chen

et al. 2013

Molecules

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Spatholobus suberectus Dunn, belonging to the legume family (Fabaceae), has been used as a Traditional Chinese Medicine for the treatment of anemia, menoxenia and rheumatism. A limited number of studies report that various types of flavonoids are the main characteristic constituents of this herb. We have now found that S. suberectus contains about 2% phenolic components and characterized the major phenolic components as homogeneous B-type procyanidin conjugates using a liquid chromatography with diode-array detection-ESI mass spectrometry (LC-DAD/ESI-MS) method. This is the first report on occurrence of most B-type procyanidins in this herb. Moreover, the total phenolics extract was assayed for inhibitory activity on human neutrophil elastase and its IC 50 was found to be 1.33 μg/mL.

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Section: Introductionmentioning

confidence: 99%

Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MSn and Their Inhibitory Effect on Human Neutrophil Elastase Activity

Huang

Chen

et al. 2013

Molecules

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“…The discrimination of these selected herbs was in agreement with the high radical scavenging activity samples especially for P. minus, P. indica and C. caudatus, which were expected to be discriminated from the others due to their high concentration of secondary metabolites, especially flavonoid. The presence of these phenolic compounds is also believed to contribute to the greater elastase and collagenase inhibitory activities of these herbs [67,68,69,70,71,72,73,74,75,76,77,78,79,89,90,91,92].…”

Section: Resultsmentioning

confidence: 99%

NMR-Based Metabolomics Profiling for Radical Scavenging and Anti-Aging Properties of Selected Herbs

et al. 2019

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Herbs that are usually recognized as medicinal plants are well known for their therapeutic effects and are traditionally used to treat numerous diseases, including aging. This study aimed to evaluate the metabolite variations among six selected herbs namely Curcurma longa, Oenanthe javanica, Vitex negundo, Pluchea indica, Cosmos caudatus and Persicaria minus using proton nuclear magnetic resonance (1H-NMR) coupled with multivariate data analysis (MVDA). The free radical scavenging activity of the extract was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC) assay. The anti-aging property was characterized by anti-elastase and anti-collagenase inhibitory activities. The results revealed that P. minus showed the highest radical scavenging activities and anti-aging properties. The partial least squares (PLS) biplot indicated the presence of potent metabolites in P. minus such as quercetin, quercetin-3-O-rhamnoside (quercitrin), myricetin derivatives, catechin, isorhamnetin, astragalin and apigenin. It can be concluded that P. minus can be considered as a potential source for an anti-aging ingredient and also a good free radical eradicator. Therefore, P. minus could be used in future development in anti-aging researches and medicinal ingredient preparations.

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“…The similar inhibitory tendency was also observed from other alkylated flavones 3 and 4, which indicated that the prenyl group on C3 was a crucial functionality to HNE inhibition. Dihydrobenzoxanthones (5)(6)(7)(8) inhibited HNE significantly with IC 50 values of 9.8 ~ 28.7 μM, compared to the mother compound. The number of the hydroxyl group of B-ring affected inhibitory capacities as follow: compounds 5 (IC 50 = 9.8 μM) versus 8 (IC 50 = 28.7 μM).…”

Section: Hne Inhibitory Activity Of Isolated Compounds and Their Kinementioning

confidence: 96%

“…The stoppage of releasing AAT in hepatocytes expressively decreases its levels, leading to emphysema because of deficient defence of the lower respiratory tract from human neutrophil elastase (HNE), and further alveoli damage [4]. This leads to the development of the chronic obstructive pulmonary disease, emphysema and lung cancer, also in the liver, it causes benign neonatal hepatitis syndrome, fibrosis which evolves to cirrhosis and even to hepatocellular carcinoma [5]. Augmentation therapy has been accepted as the best therapy for AAT deficiency, and less costly methods such as low molecular weight NE inhibitors have failed clinically, despite diligent efforts over the past three decades [6].…”

Section: Introductionmentioning

confidence: 99%

Human neutrophil elastase inhibitory dihydrobenzoxanthones and alkylated flavones from the Artocarpus elasticus root barks

et al. 2020

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Neutrophil elastases are deposited in azurophilic granules interspace of neutrophils and tightly associated with inflammatory ailments. The root barks of Artocarpus elasticus had a strong inhibitory potential against human neutrophil elastase (HNE). The responsible components for HNE inhibition were confirmed as alkylated flavones (2–4, IC50 = 14.8 ~ 18.1 μM) and dihydrobenzoxanthones (5–8, IC50 = 9.8 ~ 28.7 μM). Alkyl groups on flavone were found to be crucial functionalities for HNE inhibition. For instance, alkylated flavone 2 (IC50 = 14.8 μM) was 20-fold potent than mother compound norartocarpetin (1, IC50 > 300 μM). The kinetic analysis showed that alkylated flavones (2–4) were noncompetitive inhibition, while dihydrobenzoxanthones (5–8) were a mixed type I (KI < KIS) inhibitors, which usually binds with free enzyme better than to complex of enzyme–substrate. Inhibitors and HNE enzyme binding affinities were examined by fluorescence quenching effect. In the result, the binding affinity constants (KSV) had a significant correlation with inhibitory potencies (IC50).

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The In Vitro Inhibition of Human Neutrophil Elastase Activity by some Yemeni Medicinal Plants

Cited by 18 publications

References 33 publications

Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MSn and Their Inhibitory Effect on Human Neutrophil Elastase Activity

Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MSn and Their Inhibitory Effect on Human Neutrophil Elastase Activity

NMR-Based Metabolomics Profiling for Radical Scavenging and Anti-Aging Properties of Selected Herbs

Human neutrophil elastase inhibitory dihydrobenzoxanthones and alkylated flavones from the Artocarpus elasticus root barks

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