The in vitro induction and release of a cell toxin by immune C57B1/6 mouse peritoneal macrophages

Kramer, J. J.; Granger, Gale A.

doi:10.1016/0008-8749(72)90229-8

Cited by 64 publications

(23 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In accordance with our earlier findings (Keller, 1976a, b) the, range of target cell types differed considerably in their susceptibility to macrophage-mediated cytolysis; pointing to the important role of some as yet unidentified attributes of target cells. Earlier work had indicated that, contrary to the reports of others (Hibbs, 1974;Holtermann, Klein and Casale, 1973), there is no consistent correlation between degree of transformation and susceptibility to macrophagemediated cytocidal effects (Granger and Weiser, 1964;Kramer and Granger, 1972;McLaughling, Ruddle and Waksman, 1972;Nathan and Terry, 1975;Keller, 1974Keller, , 1976b). The present finding that primary explants from normal mouse epidermis and P815 mouse mastocytoma cells are comparably susceptible to the lytic effect, whereas SV40-transformed mouse macrophages are resistant, lends further support to the view that targetcell properties other than those associated with malignant transformation determine susceptibility to macrophage cytocidal effects.…”

Section: Fffector Capacities Of Adherent Cells From Different Rat Strmentioning

confidence: 77%

Macrophage-mediated natrual cytotoxicity against various target cells in vitro. I. Macrophages from diverse anatomical sites and different strains of rats and mice

Keller¹

1978

Br J Cancer

View full text Add to dashboard Cite

Summary.-Adherent, predominantly phagocytic, mononuclear cells expressing spontaneous cytotoxic activity against diverse target cells in vitro were present in various tissues of different strains of rats and mice. Cells with such natural killer capacity were thus everywhere readily available for mobilization and activation. The inherent spontaneous killer capacity of adherent mononuclear phagocytes can be abrogated by silica particles in vitro, and can be considerably enhanced by appropriate stimuli in vivo. Spontaneous cytolysis mediated by unstimulated mononuclear phagocytes was consistently manifested only after a lag phase of 12-20 h and was quite nonspecific; there was no cogent correlation between susceptibility to lysis and transformation.

show abstract

Section: Fffector Capacities Of Adherent Cells From Different Rat Strmentioning

confidence: 77%

Macrophage-mediated natrual cytotoxicity against various target cells in vitro. I. Macrophages from diverse anatomical sites and different strains of rats and mice

Keller¹

1978

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…The adherent continuous lines used were L-929 cells, referred to as L-cells (17); HeLa, human cervical carcinoma; HT -1080, human skin fibrosarcoma; Chang, human liver carcinoma; T -24, human bladder carcinoma; \. ESH-7, a cell line derived from the fusion between HeLa .…”

Section: Methodsmentioning

confidence: 99%

“…a) Tube assay: The details of this method have been reported previously (17). Briefly, I X 105 mitomycin target cells in 1.0 ml were established as nondividing monolayers in l6X125-mm culture tubes.…”

Section: Methodsmentioning

confidence: 99%

Isolation and Initial Characterization of Tumoricidal Monokine(s) From the Human Monocytic Leukemia Cell Line THP-1<xref ref-type="fn" rid="FN2">2</xref>

1985

JNCI: Journal of the National Cancer Institute

View full text Add to dashboard Cite

-A cloned subline of the human monocytic leukemia cell line, THP-1, was induced to produce high levels of cytotoxic activity following an 18-hour phorbol myristate acetate (CAS: 16561-29-8) stimulation in vitro. This activity, termed monocyte cell line cytotoxin(s) (MCCT), was tested in vitro on different human continuous cell lines (Chang, ESH-7, GM3104A, HeLa, HT-1080, K562, Mel, T-24) and on primary human fibroblasts (GM3468, Manz). The continuous cell lines exhibited a spectrum of sensitivity to MCCT-containing supernatants whereas the primary fibroblasts were resistant to lysis. Enzymatic degradation and heat denaturation studies indicate that MCCT is a protein. Its bioactivity can be resolved into three lytic peaks after molecular sieving on Ultrogel AcA 44. The major peak, designated aMCCT, eluted with a molecular weight of 100,000-140,000 daltons. A minor peak, {3MCCT, was seen at 60,000-80,000 daltons, and a third, unstable minor peak,,,MCCT, eluted at less than 10,000 daltons. The a-lytic peak was examined further and was found to migrate as a single peak in 7% native polyacrylamide gel electrolysis tube gels with an r, of 0.25-0.30. None of the MCCT forms were immunologically cross-reactive with human a-Iymphotoxin. Various protease inhibitors known to inhibit monokine-and macrophage-mediated direct cell lysis in vitro were tested for their inhibitory effects on aMCCT activity. The irreversible binding inhibitor Na-p-tosyl-L-Iysyl chloromethyl ketone inhibited the biologic activity of aMCCT. The reversible binding inhibitors Na-p-tosyl-L-arginine methyl ester and soybean trypsin inhibitor were able to block in vitro lytic activity when added to aMCCT in the presence of the target cell. In contrast, the inhibitors phenylmethylsulfonyl fluoride, L-1-tosylamide, 2-phenylethyl chloromethyl ketone, and Na-acetyl-L-Iysine methyl ester were not effective in blocking cytolysis. Finally, the hydrogen peroxide scavenger catalase inhibited aMCCT lytic activity in vitro; however, the hypochlorous acid scavengers alanine, serine, and valine were without effect. -JNCI 1985; 74:1-9. Human peripheral blood monocytes and alveolar macrophages can be raised to a cell-lytic state by treatment in vitro with lymphokines and/or bacterial components (1-5). These activated human mononuclear cells exhibit a greater capacity to lyse neoplastic cells rather than primary cells in vitro. In addition, recent reports have demonstrated the release of eLM from peripheral blood monocyte and alveolar macrophage cultures [(6-8); Klostergaard J: In preparation; Sone S, Lopez-Dertein G, Fidler IJ: In preparation). Supernatants containing eLM from these cultures were also found in general to preferentially lyse continuous cells over primary cells in vitro.For the in-depth investigation of eLM, the production of large amounts of human supernatant would be necessary from a homogeneous monocyte-macrophage source, preferably from a single donor. Primary cultures 1 of monocytes from many individual donors do not fulfill these crit...

show abstract

“…Macrophages and certain continuous macrophage-like cell lines, from experimental animals and humans, can be stimulated to release materials with MCT activity in vitro (2,3). However, these effector cells and cell lines must first be raised to a tumoricidal state of activation before they can be stimulated to release MCT.…”

Section: Macrophage Cytotoxic Factorsmentioning

confidence: 99%

“…It has been known for a number of years that activated lymphocytes and macrophages from experimental animals and humans can be stimulated in vitro to release materials that are growth inhibitory and lytic for nucleated mammalian cells in vitro, Materials with these activities were termed lymphotoxins (LT) and macrophage cytotoxins (MCT), respectively (1,2). It is also evident that serum from bacillus Calmette Guerin (BCG)-infected and endotoxin-challenged animals contains a material(s) that causes selective necrosis of murine tumor's when injected into tumor-bearing mice.…”

Section: Introductionmentioning

confidence: 99%

Lymphotoxins, macrophage cytotoxins, and tumor necrosis factors: An interrelated family of antitumor effector molecules

1985

View full text Add to dashboard Cite

The in vitro induction and release of a cell toxin by immune C57B1/6 mouse peritoneal macrophages

Cited by 64 publications

References 14 publications

Macrophage-mediated natrual cytotoxicity against various target cells in vitro. I. Macrophages from diverse anatomical sites and different strains of rats and mice

Macrophage-mediated natrual cytotoxicity against various target cells in vitro. I. Macrophages from diverse anatomical sites and different strains of rats and mice

Isolation and Initial Characterization of Tumoricidal Monokine(s) From the Human Monocytic Leukemia Cell Line THP-1<xref ref-type="fn" rid="FN2">2</xref>

Lymphotoxins, macrophage cytotoxins, and tumor necrosis factors: An interrelated family of antitumor effector molecules

Contact Info

Product

Resources

About