Macrophage-mediated natrual cytotoxicity against various target cells in vitro. I. Macrophages from diverse anatomical sites and different strains of rats and mice

Keller, R.

doi:10.1038/bjc.1978.111

Cited by 82 publications

(39 citation statements)

References 31 publications

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“…The findings in Table I, which summarizes the results of a series of parallel experiments, clearly show that, despite considerable scatter from one experiment to another, adherent predominantly phagocytic peritoneal cells from normal DA rats express natural cytotoxicity against a variety of target cells; the data thus confirm and extend earlier observations (Keller, 1976c(Keller, , 1978Keller and Keist, 1978). Effector cells with such capacities were present in every age group examined.…”

Section: Resultssupporting

confidence: 85%

“…injection of 10 ml of 10% proteose peptone, were seeded into 35 x 10 mm Corning plastic Petri dishes as described in Keller (1978) and Keller and Keist (1978). After culture at 37°C in a humid atmosphere of 5% C02 and 95% air in RPMI-1640 (Keller, 1976a;Keller, 1978).…”

Section: Methodsmentioning

confidence: 99%

“…After culture at 37°C in a humid atmosphere of 5% C02 and 95% air in RPMI-1640 (Keller, 1976a;Keller, 1978).…”

Section: Methodsmentioning

confidence: 99%

“…Target cells.-Apart from target cell types used in previous work (Keller, 1978), polyomavirus-induced tumour cells from DA rats (Keller, 1973;Keller and Keist, 1978) were also included in this study. All targets were grown in RPMI-1640 medium supplemented with 10% foetal calf serum (FCS; Gibco, Grand Island, N.Y.).…”

Section: Methodsmentioning

confidence: 99%

“…PREVIOUS work has shown that adherent phagocytic cells capable of mediating spontaneous cytotoxicity against a variety of nucleated target cells were present in various normal nonstimulated tissues such as spleen, lungs, peritoneum and marrow of different strains of rats and mice (Keller, 1978). This study examines the ontogeny of adherent phagocytic cells with natural killer activity in rat tissues.…”

mentioning

confidence: 96%

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Macrophage-mediated natural cytotoxicity against various target cells in vitro. II. Macrophages from rats of different ages

Keller¹

1978

Br J Cancer

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Summary.-Adherent, predominantly phagocytic mononuclear cells from various rat tissues express spontaneous cytotoxicity against diverse target cells in vitro. The extent to which cytotoxicity was expressed by effector cells depended on the age of donors. Cytolytic effector-cell capacity was already fully developed a few days after birth, and persisted over many months, but was clearly reduced in senescence. Similarly, the ability to intensify natural cytotoxicity by peptone in vivo was already fully manifested in the newborn, but was significantly diminished in old rats.

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Section: Resultssupporting

confidence: 85%

Section: Methodsmentioning

confidence: 99%

“…After culture at 37°C in a humid atmosphere of 5% C02 and 95% air in RPMI-1640 (Keller, 1976a;Keller, 1978).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 96%

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Macrophage-mediated natural cytotoxicity against various target cells in vitro. II. Macrophages from rats of different ages

Keller¹

1978

Br J Cancer

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Interferon‐induced enhancement of macrophage‐mediated tumor cytolysis and its difference from activation by lymphokines

Boraschi

Tagliabue

1981

Eur J Immunol

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Mouse peritoneal macrophages expressed increased cytolytic activity against tumor cells upon in vitro exposure to partially purified L cell interferon (IFN-beta). In contrast, treatment with human IFN or with mock IFN preparations did not enhance macrophage tumoricidal capacity. Macrophage activation by IFN was optimal with long exposure times and high IFN concentrations. Treatment with polymyxin B sulfate did not affect IFN-induced macrophage cytotoxicity, thus excluding the possibility that bacterial lipopolysaccharide contaminants were responsible for macrophage activation. Conversely, treatment with a highly specific anti-IFN antiserum completely abolished IFN effect on macrophages, but had no effect on lymphokine-induced cytolysis. IFN and the lymphokine macrophage-activating factor (MAF) were compared for their ability to provide the sequence of activation signals to macrophages from the normal responder C3H/HeN mice and from C3H/HeJ mice, which are defective for several macrophage responses. Like MAF, IFN was incapable of inducing tumoricidal activity in C3H/HeJ macrophages. However, whereas MAF provided the first "priming" signal to macrophages of both strains, IFN acted as first signal only for C3H/HeN macrophages, being inactive for cells of the defective C3H/HeJ strain. Furthermore, IFN was not capable of providing the second "expression" signal to "primed" macrophages. These data suggest two different macrophage activation pathways for IFN and MAF.

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Temperature and cellular regulation of spontaneous cytotoxicity in the shark

Pettey

McKinney

1983

Eur J Immunol

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Spontaneous cytolytic activity of shark peripheral blood leukocytes is observed only during periods of decreased environmental temperature (less than 23 degrees C). The effector cell is adherent to glass and is phagocytic. Leukocytes tested during warmer periods (26-31 degrees C) exhibit no spontaneous activity; however, glass-adherent cells isolated from those fish are cytotoxic in vitro, indicating that the effector cell is present at all temperatures. During warmer temperatures, nonadherent cells added to adherent cells were shown to inhibit spontaneous cytotoxicity. This inhibition requires viable cells in contact with the spontaneous cytotoxic population. Thus decreased environmental temperature correlated with spontaneous cytotoxicity, and appears to affect a regulatory cell that is glass nonadherent. In addition, the cytotoxic effector cell is more active at 23 degrees C than 30 degrees C in vitro. These data show that by the time of emergence of the nurse shark, a temperature-dependent mechanism had evolved for cellular regulation of at least one immune function, spontaneous cytotoxicity.

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Macrophage-mediated natrual cytotoxicity against various target cells in vitro. I. Macrophages from diverse anatomical sites and different strains of rats and mice

Cited by 82 publications

References 31 publications

Macrophage-mediated natural cytotoxicity against various target cells in vitro. II. Macrophages from rats of different ages

Macrophage-mediated natural cytotoxicity against various target cells in vitro. II. Macrophages from rats of different ages

Interferon‐induced enhancement of macrophage‐mediated tumor cytolysis and its difference from activation by lymphokines

Temperature and cellular regulation of spontaneous cytotoxicity in the shark

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