The in vitro characterization of a gelatin scaffold, prepared by cryogelation and assessed in vivo as a dermal replacement in wound repair

Shevchenko, Rostislav V.; Eeman, Marc; Rowshanravan, Behzad; Allan, Iain; Savina, Irina N.; Illsley, Matthew; Salmon, Michel; James, Stuart L.; Mikhalovsky, Sergey V.; James, Stuart

doi:10.1016/j.actbio.2014.03.027

Cited by 48 publications

(47 citation statements)

References 50 publications

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“…Gelatin is an irreversibly denatured form of collagen with the ability to form a scaffold suitable for dermal regeneration without the incorporation of any other polymers[69]. However as previously mentioned, gelatin only scaffolds have decreased fibroblast migration compared to collagen-based scaffolds[15,16].…”

Section: Components Of a Skin Substitutementioning

confidence: 99%

“…This will cause cells to adhere on the surface of the scaffold as the cells settle downwards due to gravity, however this may lead to suboptimal concentrations of cells adhering to the scaffold. An additional method would be growing cells on a surface, placing one side of the scaffold on the surface, and then allowing time for the cells to migrate and adhere to the scaffold[206]. Yet this may require excess time to allow cells to fully migrate and adhere to the scaffold.…”

Section: Creating Skin Substitutesmentioning

confidence: 99%

“…The challenge is seeding two cell lines to create a bilayer skin substitute as there needs to be a clear division between epidermal and dermal layers. After culturing scaffolds with adhered dermal cells, it is possible to seed epidermal cells dropwise on to one surface of the scaffold[214] or by placing the cellularized scaffold on to a surface previously adhered with keratinocytes[206]. Both of these techniques may create a bilayer scaffold, but one significant component is missing, the basement membrane.…”

Section: Creating Skin Substitutesmentioning

confidence: 99%

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Methodologies in creating skin substitutes

Nicholas

Jeschke

Amini-Nik

2016

Cell. Mol. Life Sci.

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The creation of skin substitutes has significantly decreased morbidity and mortality of skin wounds. Although there are still a number of disadvantages of currently available skin substitutes, there has been a significant decline in research advances over the past several years in improving these skin substitutes. Clinically most skin substitutes used are acellular and do not use growth factors to assist wound healing, key areas of potential in this field of research. This article discusses the five necessary attributes of an ideal skin substitute. It comprehensively discusses the three major basic components of currently available skin substitutes: scaffold materials, growth factors, and cells, comparing and contrasting what has been used so far. It then examines a variety of techniques in how to incorporate these basic components together to act as a guide for further research in the field to create cellular skin substitutes with clinically better results.

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Section: Components Of a Skin Substitutementioning

confidence: 99%

Section: Creating Skin Substitutesmentioning

confidence: 99%

Section: Creating Skin Substitutesmentioning

confidence: 99%

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Methodologies in creating skin substitutes

Nicholas

Jeschke

Amini-Nik

2016

Cell. Mol. Life Sci.

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“…This system consists of a collagen-GAG scaffold prepared by a freeze-drying process, with an attached silicone pseudoepidermal layer for wound-repair purposes. The scaffold features an interconnected macroporous structure with a pore-size distribution of 131 ± 17 μm on one surface, decreasing to 30 ± 8 μm on the attached silicone surface (57).…”

Section: Wound Model In Micementioning

confidence: 99%

PKCδ inhibition normalizes the wound-healing capacity of diabetic human fibroblasts

Khamaisi¹,

Katagiri²,

Keenan³

et al. 2016

Journal of Clinical Investigation

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“…In the most classical approach, known as scaffold-based or top-down, scaffolds are fabricated through either conventional or additive manufacturing processes, and subsequently seeded with autologous or allogeneic cells. The cellular scaffold is then cultured in vitro to produce a tissue-engineered construct for subsequent implantation into the lesion site [66,116,146,177]. Despite the fact that such an approach allows for a good control over the scaffold characteristics (when additive manufacturing processes are used) and cellscaffold interactions, it fails in placing individual cells at specific locations throughout the scaffold, thereby not mimicking the intricate cellular organization of natural tissues at micro-and nanoscale.…”

Section: Introductionmentioning

confidence: 99%

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

et al. 2017

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Bioprinting technologies are powerful additive biofabrication techniques to produce cellular constructs for skin tissue engineering owing to their unique ability to precisely pattern living and non-living materials in predefined spatial locations. This unique feature, combined with the computer controlled printing and medical imaging techniques, enable researchers and clinicians to generate patient specific constructs partly replicating the intricate compositional and architectural organization of the skin. Bioprinting has been used to automatically dispense hydrogels with skin cells located in prescribed sites that promote skin formation in vitro and in vivo. Current skin bioprinting approaches mostly rely on the sequential printing of fibroblasts and keratinocytes embedded within a homogeneous hydrogel. Although such approaches have already been translated to pre-clinical scenarios, they still present limitations in terms of fully replicating the cellular and extracellular matrix (ECM) heterogeneity in native skin. The success of bioprinting for skin repair strongly depends on the design of printable bioinks capable of supporting the function of printed cells and stimulating the production of new ECM components. To better mimic the human skin, novel developments in dedicated bioprinting technologies, in the design of bioinks, as well as in the printing of vascularised constructs are necessary. This paper presents an overview regarding the use of bioprinting for skin tissue engineering applications. The operating principles of bioprinting technologies are outlined along with requirements of printed skin constructs. Finally, preclinical results are summarized and future perspectives for the field are highlighted.

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The in vitro characterization of a gelatin scaffold, prepared by cryogelation and assessed in vivo as a dermal replacement in wound repair

Cited by 48 publications

References 50 publications

Methodologies in creating skin substitutes

Methodologies in creating skin substitutes

PKCδ inhibition normalizes the wound-healing capacity of diabetic human fibroblasts

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

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