The improved Allium/Vicia root tip micronucleus assay for clastogenicity of environmental pollutants

Ma, Te­-Hsiu; Xu, Zhidong; Xu, C.; McConnell, Heike; Rabago, Eugenia Valtierra; Arreola, Gemma Adriana; Zhang, Hongen

doi:10.1016/0165-1161(95)90010-1

Cited by 273 publications

(149 citation statements)

References 13 publications

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“…The centric fragments or laggards being omitted from the nucleus proper during mitosis further lead to development of micronuclei. Such a rise in micronuclei is also suggestive of the abilities of test chemicals in producing different types of CAs (Ma et al, 1995). The results from this study showed a statistically significant difference in the frequencies of micronuclei induction in mice bone marrow exposed to AFB1 when compared to the control.…”

Section: Micronuclei Inductionsupporting

confidence: 54%

Assessment of Genotoxic and Cytogenetic Effects of Aflatoxin B1 in Mouse Bone Marrow Cells

Algarni¹

2018

Int.J.Curr.Microbiol.App.Sci

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Section: Micronuclei Inductionsupporting

confidence: 54%

Assessment of Genotoxic and Cytogenetic Effects of Aflatoxin B1 in Mouse Bone Marrow Cells

Algarni¹

2018

Int.J.Curr.Microbiol.App.Sci

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“…The in-duction of laggards could be attributed to the failure of spindle apparatus to organize and function in a normal way (Patil and Bhat 1992). As a rule, micronucleus formation is the result of acentric fragments or laggards being excluded from the nucleus during mitosis (Ma et al 1995). The induction of micronucleus and multipolarity indicate the mutagenic potential of the applied polyamines.…”

Section: Resultsmentioning

confidence: 99%

Effects of Polyamines (Putrescine, Spermidine and Spermine) on Root Tip Mitosis and Chromosomes in Allium cepa L.

2005

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SummaryIn the present study effects of the polyamines; putrescine (Put), spermidine (Spd) and spermine (Spm) have been studied on root tip mitosis of Allium cepa L. Root tips of A. cepa were treated with a series of polyamine (PA) concentrations, ranging from 0.01, 1 and 2 mM for 3, 6, 12, 24 and 48 h. The roots were examined in permanent root tip squash preparations stained by the Feulgen technique. This research has confirmed that PAs have various effects on chromosomes. PA concentrations used, generally had a marked mitodepressive effect on mitotic index (MI) but at 6 h treatment of Spm, Spd and Put at all concentrations had increased MI. Polyamines affected the relative duration of each mitotic stage as compared to the control. They also increased frequency of abnormal mitosis.The types of abnormalities induced are: chromosome stickiness, c-metaphase, anaphase and telophase bridges, disturbed chromosomes of anaphase and telophase stages, lagging and forward chromosomes at anaphase and telophase. Binucleate cells and micronuclei formation at interphase cells were also observed.

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“…The whole tip meriste-. matic and F1 regions was preferred to only F1 as w x suggested by Ma et al 16 to avoid to introduce an error in micronucleus estimation due to mistakes in choosing the region to be analysed. Scoring for counting a MN was based on generally accepted w x criteria for MN identification 17 .…”

mentioning

confidence: 99%

Mutagenicity (micronucleus test in Vicia faba root tips), polycyclic aromatic hydrocarbons and heavy metal content of sediments collected in Tiber river and its tributaries within the urban area of Rome

Minissi

Caccese

Passafiume

et al. 1998

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Sediments collected in Tiber river and in its main tributary water courses within the urban area of Rome were tested for Ž . mutagenicity by means of Vicia faba root tips micronucleus MN test. Representative samples were scored for Ž . micronucleus generating events chromosomerchromatid loss and fragments too. Sediments were assayed for content of the Ž . Ž thirteen most important chemicals of polycyclic aromatic hydrocarbon PAH group and for some heavy metal ions Cd, Cr, . Ž . Cu, Ni, Pb, Zn . Samples were collected in four tributary rivers Prima Porta, Acqua Traversa, Aniene and Magliana just before their confluence with Tiber river and at different stations along the Tiber river itself upstream and downstream the sites of confluence of the sampled tributaries. All samples were collected in July 1992. An alarming level of mutagenicity was reached in most of the tested stations, with an effect comparable to an X-rays exposure up to 0.4 Gy. Chemical analysis showed that the total amount of identified PAHs ranged from 4.5 to 625.2 ngrg of dry matrix in the different stations and the total amount of heavy metals ranged from 130 to 570 ppm. Tiber mutagenicity is likely to be mainly due to local factors such as the confluence of a small polluted tributary rather than to large scale effect due to an upstream-downstream relationship. q

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The improved Allium/Vicia root tip micronucleus assay for clastogenicity of environmental pollutants

Cited by 273 publications

References 13 publications

Assessment of Genotoxic and Cytogenetic Effects of Aflatoxin B1 in Mouse Bone Marrow Cells

Assessment of Genotoxic and Cytogenetic Effects of Aflatoxin B1 in Mouse Bone Marrow Cells

Effects of Polyamines (Putrescine, Spermidine and Spermine) on Root Tip Mitosis and Chromosomes in Allium cepa L.

Mutagenicity (micronucleus test in Vicia faba root tips), polycyclic aromatic hydrocarbons and heavy metal content of sediments collected in Tiber river and its tributaries within the urban area of Rome

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