The importance of the explant on regeneration in thin cell layer technology

Nhựt, Dương Tấn; Silva, Jaime A. Teixeira da; Aswath, C.

doi:10.1079/ivp2002408

Cited by 34 publications

(22 citation statements)

References 31 publications

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“…Morphogenic response (%) When medium-dependent tTCLs (with limited sugar reserves and no defined sink-source) are used, heterotrophy or mixotrophy is forced by the addition of sugar to the culture medium (NHUT et al, 2003). Depending on the light intensity, temperature and carbon dioxide concentration in the Petri-dish air, in vitro plant growth and development can be totally dependent on an external source of carbohydrates (tTCL cultures in the dark) or partly covered their needs by limited photosynthesis (tTCL cultures in the light; NHUT et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Depending on the light intensity, temperature and carbon dioxide concentration in the Petri-dish air, in vitro plant growth and development can be totally dependent on an external source of carbohydrates (tTCL cultures in the dark) or partly covered their needs by limited photosynthesis (tTCL cultures in the light; NHUT et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…TCLs, derived from tissues or organs, are small in size, and are excised either a) longitudinally (lTCL), being thus composed of a few tissue types or b) transversally (tTCL), thus composed of several tissue types, which are, however, normally too small to separate, as in the case of chrysanthemum. In the TCL system, the morphogenic and developmental pathways of specific organs may be clearly directed and controlled (NHUT et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Among other potentially important applications of chrysanthemum transformation, the most vital is that of engineering virus/viroid resistance (TEIXEIRA DA SIL-VA, 2003c). Furthermore, the shoot regeneration capacity of chrysanthemum is severely hampered by the presence of antibiotics in the selective caulogenic medium, which, despite optimisation for maximum shoot production in a caulogenic program, is disturbed at higher antibiotic concentrations (TEIXEIRA DA SILVA et al, 2003). Thus, the search for alternative selection systems using other carbon sources may benefit the outcome and efficiency of chrysanthemum genetic transformation.…”

Section: Introductionmentioning

confidence: 99%

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The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layers

Silva

2004

Bragantia

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Carbon source is an indispensable factor for the development of an in vitro morphogenic program of chrysanthemum micropropagation. The choice of carbon source affects the qualitative and quantitative outcome, and also the response of thin cell layers when these are placed onto morphogenic (callus, root, shoot, somatic embryo) media. Threshold survival levels (TSLs) could not be obtained for sucrose, fructose or glucose. TSLs together with organ differentiation were, however, obtained for mannose (60-80 g.L -1 ), xylose and lactose (40 g.L -1 ) and cellulose (60-80 g.L -1 ), making these carbon sources suitable as potential carbon sources for positive selection systems for chrysanthemum genetic transformation.Key words: Dendranthema X grandiflora, phytotoxicity, regeneration capacity, threshold survival level. RESUMO EFEITO DE CARBONO NA ORGANOGÊNESE DE CAMADAS CELULARES FINAS DE CRISÂNTEMOA fonte de carbono é factor indispensável no sucesso de um programa de desenvolvimento morfogênico in vitro de crisântemo. A escolha da fonte de carbono afeta a resposta qualitativa e quantitativa de camadas celulares finas, quando são colocadas em meios de desenvolvimento morfogênico (calo, raiz, eixo caulinar, embrião somático). Não se estabeleceram níveis de sobrevivência quando sucrose, frutose ou galactose foram usados, mas sim para manose (60-80 g.L -1 ), xilose e lactose (40 g.L -1 ), e celulose (60-80 g.L -1 ), sugerindo o potencial dessas fontes de carbono em sistemas de seleção positivas em programas de transformação genética de crisântemo.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layers

Silva

2004

Bragantia

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“…LEDs have also been used to study the effect of light intensity and wavelength on the growth and development of several species. This work has included improvement of in vitro photoautotrophic growth of Cymbidium (Tanaka et al, 1998), improvement of in vitro growth of strawberry (Nhut et al, 2003), and study of morphogenesis and growth of Lilium bulb scales (Lian et al, 2002) under different combinations of blue and red LEDs. Other examples include studies of growth and petiole morphogenesis in Zantedeschia under LEDs (Jao et al, 2005), and improvement of growth and carbohydrate biosynthesis of grapes exposed to LEDs (Heo et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Induction, Growth and Paclitaxel Content of Needle-and Petiole-Derived Calli in Himalayan Yew (Taxus Wallichiana Zucc.) Under Light-Emitting Diodes

Nhựt

Nguyen

Don

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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To achieve a better understanding of accumulation of paclitaxel and related taxanes in Himalayan yew (Taxus wallichiana Zucc.) callus mass, induction and growth of calli from needles and petioles under light-emitting diodes (LEDs) and production of paclitaxel and baccatin were investigated. Calli were induced from needles and petioles on Gamborg's B5 medium supplemented with 1 mg·l -1 kinetin and 4 mg·l -1 2,4-D under different combinations of blue and red LEDs. Calli were further cultured in that medium under the same lighting conditions. Callus induction from needles and petioles and growth was better under blue LEDs than under red LEDs and fluorescent light. The combinations of blue and red LEDs produced variable results. The paclitaxel content of these calli was quantified by high-performance liquid chromatography. The paclitaxel content of calli derived from needles was 0.00628% and the 10-DAB (10-Deacetylbaccatin III) content was 0.00366%. The paclitaxel content of calli derived from petioles was 0.00412%, and no 10-DAB was detected.

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