The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layers

Silva, Jaime A. Teixeira da

doi:10.1590/s0006-87052004000200002

Cited by 26 publications

(13 citation statements)

References 19 publications

(10 reference statements)

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“…Carbohydrates acted as signaling particles in all phases of plant development. Silva ( 2004 ) and Ahmad et al ( 2007 ) indicated that sugars are perceived by the cell as chemical signals in vitro , with very high concentrations acting as stressing agents. Perata et al ( 1997 ) reported that high sugar concentrations could inhibit gibberellin signaling and suppress cell division and growth in several different plant systems.…”

Section: Discussionmentioning

confidence: 99%

In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers

Ilczuk

Jacygrad

2016

In Vitro Cell.Dev.Biol.-Plant

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Cornus alba L. (white dogwood) is an important ornamental shrub having a wide range of applications such as reforestation programs and soil retention systems. The vegetative propagation of dogwood by cuttings may be slow, difficult, and cultivar dependent; therefore, an improved micropropagation method was developed. Nodal stem segments of C. alba cultivars ‘Aurea’ and ‘Elegantissima’ were cultured on media enriched with six different sources of macronutrients. Media were supplemented with either N6-benzyladenine (BA) or thidiazuron (TDZ) in combination with 1-naphthaleneacetic acid (NAA). Regardless of the cultivar, the best shoot proliferation was observed on Lloyd and McCown medium (woody plant medium (WPM)) at pH 6.2, containing 1.0 mg L−1 BA, 0.1 mg L−1 NAA, and 20–30 g L−1 sucrose. Rooting of regenerated shoots was achieved by an in vitro method when different concentrations of NAA or indole-3-butyric acid (IBA) were tested. Microcuttings were rooted for 8 wk on medium enriched with 0.25 mg L−1 NAA and potted into P9 containers in the greenhouse. The final survival rate of the plants after 20 wk was 80% for ‘Aurea’ and 90% for ‘Elegantissima’. Genetic stability of the micropropagated plants was confirmed by using two DNA-based molecular marker techniques. A total of 30 random amplified polymorphic DNA (RAPD) and 20 inter-simple sequence repeat (ISSR) primers resulted in 197–199 and 184–187 distinct and reproducible band classes, respectively, in ‘Aurea’ and ‘Elegantissima’ plantlets. All of the RAPD and ISSR profiles were monomorphic and comparable with the mother plant.

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Section: Discussionmentioning

confidence: 99%

In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers

Ilczuk

Jacygrad

2016

In Vitro Cell.Dev.Biol.-Plant

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“…Hal ini dimungkinkan karena tunas yang terenkapsulasi mengalami penghambatan dalam penyerapan hara dan respirasi, sehingga media dengan kandungan sumber karbon yang tinggi lebih mendukung pertumbuhan kultur. Menurut Da Silva (2004), sukrosa merupakan salah satu jenis gula yang siap dimetabolismekan selain glukosa dan fuktosa. Jenis gula demikian akan lebih mudah diuraikan dalam sel tanaman sehingga sangat mendukung pertumbuhan kultur.…”

Section: Hasil Dan Pembahasan Perlakuan Prakulturunclassified

Kriopreservasi Tanaman Obat Langka Purwoceng dengan Teknik Enkapsulasi-Vitrifikasi

Roostika¹,

Rahayu²,

Sunarlim³

2016

BPN

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Pruatjan (Pimpinella pruatjan Molk.) is an Indonesian endangered medicinal plant, so that it is highly protected. Cryopreservation can be applied to this plant for long-term preservation. The aim of this research was to obtain a method of encapsulation-vitrification by optimizing each step in cryopreservation protocol i.e. preculture, loading, dehydration with and without freezing in liquid nitrogen. The best treatment of each step would be applied in the following step. On preculture experiment, in vitro shoots were planted on the Driver and Kuniyaki (DKW) basal media containing 0.3 M sucrose and incubated for 1, 2, 3, 4, and 5 days. After those incubation period, shoot tips were encapsulated with 2.5% Na-alginate and soaking for 15 minutes in 100 ppm CaCl 2 solution before planting. On loading experiment, precultured explants were loaded in DKW basal solution containing 2 M glycerol and 0.4 M sucrose for 0, 30, 60, and 90 minutes. On dehydration experiment, preculturead and loaded explants were dehydrated with PVS2 solution PVS2 (DKW + 30% glycerol + 15% DMSO + 15% ethyleneglicol + 0.4 M sucrose) for 0, 30, 60, 90, and 120 minutes. The parts of them were freezed in liquid nitrogen (-196 o C). The result showed that cryopreservation through encapsulation-vitrification technique could be applied on pruatjan. The best preculture treatment was 5 days incubation period. The best loading treatment was 30 minutes. The best dehydration treatment was 90 minutes. The successful level of this research was still low (10%) so that it needs optimization method.Key words: Pimpinella pruatjan Molk., cryopreservation, encapsulation-vitrification. ABSTRAKPurwoceng (Pimpinella pruatjan Molk.) adalah tanaman obat langka asli Indonesia yang hampir punah sehingga harus dilindungi. Kriopreservasi dapat diterapkan pada tanaman ini untuk penyimpanan jangka panjang. Tujuan penelitian adalah untuk memperoleh teknik enkapsulasi-vitrifikasi dengan melakukan optimasi dari tiap-tiap tahapan kriopreservasi yang meliputi perlakuan prakultur, loading, dehidrasi sebelum dan setelah pembekuan dalam nitrogen cair. Perlakuan yang terbaik kemudian diterapkan pada tahapan percobaan berikutnya. Pada perlakuan prakultur, tunas in vitro ditanam pada media Driver dan Kuniyaki (DKW) dengan penambahan sukrosa 0,3 M dengan masa inkubasi 1, 2, 3, 4, dan 5 hari. Setelah itu, pucuk yang berukuran 0,5 cm dienkapsulasi dengan Na-alginat 2,5% (yang mengandung media regenerasi) dalam larutan CaCl 2 100 ppm selama 15 menit sebelum penanaman kembali. (-196 o C) dan sebagian lainnya tidak dibekukan. Hasil penelitian menunjukkan bahwa kriopreservasi secara enkapsulasi-vitrifikasi berpeluang diterapkan pada tanaman purwoceng. Perlakuan prakultur terbaik adalah 5 hari. Perlakuan loading terbaik adalah 30 menit dan perlakuan dehidrasi terbaik 90 menit. Tingkat keberhasilan ini masih rendah (10%) sehingga diperlukan optimasi metode.Kata kunci: Pimpinella pruatjan Molk., kriopreservasi, enkapsulasi-vitrifikasi.

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“…Diduga bahwa pemberian manitol sebagai sumber karbon tidak cukup mendukung pertumbuhan kultur purwoceng. Hal yang serupa juga terjadi pada transverse thin cell layers tanaman krisan di mana penggunaan manitol sebagai sumber karbon sangat tidak efektif jika dibandingkan dengan penggunaan sukrosa, glukosa, fruktosa, maltosa, manosa, galaktosa, rafinosa, sorbitol, furanosa, selulosa, silosa, dan laktosa (Da Silva 2004). Fenomena demikian sebenarnya bersifat positif bagi upaya penyimpanan in vitro kultur purwoceng karena penghambatan pertumbuhan berpeluang untuk memperpanjang masa simpan.…”

Section: Hasil Dan Pembahasanunclassified

“…Taraf sukrosa tampak berpengaruh terhadap pertumbuhan kultur. Menurut Da Silva (2004), sukrosa merupakan salah satu jenis gula yang siap dimetabolismekan selain glukosa dan fuktosa. Jenis gula demikian akan lebih mudah diuraikan dalam sel tanaman sehingga sangat mendukung pertumbuhan kultur.…”

Section: Hasil Dan Pembahasanunclassified

Pengaruh Sumber Karbon dan Kondisi Inkubasi terhadap Pertumbuhan Kultur In Vitro Purwoceng (Pimpinella pruatjan Molk.)

2016

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Pruatjan (Pimpinella pruatjan Molk.) is an Indonesian medicinal plant which is categorized as endangered plant and included in Appendix I based on CITES. The in vitro conservation techniques have been studied. However, the storage period was very short (4 months) when plant growth retardant and media dilution were applied. Beside that, the residual effect of growth retardant was strong enough so that it needed more than 4 months for recovery. Thus, the use of certain carbon source may prolong the preservation period with shorter time for recovery. The objective of the study was to know the effects of carbon sources (sucrose and mannitol) and culture conditions (culture room and growth chamber) to the growth of pruatjan cultures. This application was hoped to prolong preservation period of pruatjan longer than 4 months and to cut the recovery period after presservation. The study was conducted at Tissue Culture Laboratory in Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development from August 2006 to July 2007. The activities included propagation of in vitro shoot grown in vitro as explants source, preservation of in vitro shoots of pruatjan, and regeneration of the cultures after preservation. The experiment was designed as factorial in Randomized Completely Block Design with 6 replications. The DKW basal media containing 1 ppm BA, 0.2 ppm thidiazuron, and 100 ppm arginine were supplemented with mannitol or sucrose at the level of 1, 2, 3, 4, and 5%. The observed variables were total number of leaves, number of shoot, and number of wilt leaves. The result revealed that pruatjan cultures could be stored longer than 4 months. Generally, the effect of mannitol or sucrose was more dominant than that of cultures condition. The mannitol (1-5%) strongly inhibited the growth of pruatjan cultures so that only a few cultures survived at 7 months preservation period and needed about 1 month for recovery. On the contrary, the effect of sucrose (at the same level) was better than mannitol. The 2.5% sucrose optimally inhibited pruatjan cultures. At that condition, the cultures could be stored for 10 months without morphological changes so that they could recover spontaneously.

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The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layers

Cited by 26 publications

References 19 publications

In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers

In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers

Kriopreservasi Tanaman Obat Langka Purwoceng dengan Teknik Enkapsulasi-Vitrifikasi

Pengaruh Sumber Karbon dan Kondisi Inkubasi terhadap Pertumbuhan Kultur In Vitro Purwoceng (Pimpinella pruatjan Molk.)

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