Pengaruh Sumber Karbon dan Kondisi Inkubasi terhadap Pertumbuhan Kultur In Vitro Purwoceng (Pimpinella pruatjan Molk.)

Abstract

<p>Pruatjan (Pimpinella pruatjan Molk.) is an Indonesian<br />medicinal plant which is categorized as endangered<br />plant and included in Appendix I based on CITES. The in<br />vitro conservation techniques have been studied. However,<br />the storage period was very short (4 months) when plant<br />growth retardant and media dilution were applied. Beside<br />that, the residual effect of growth retardant was strong<br />enough so that it needed more than 4 months for recovery.<br />Thus, the use of certain carbon source may prolong the<br />preservation period with shorter time for recovery. The<br />objective of the study was to know the effects of carbon<br />sources (sucrose and mannitol) and culture conditions (culture<br />room and growth chamber) to the growth of pruatjan<br />cultures. This application was hoped to prolong preservation<br />period of pruatjan longer than 4 months and to cut the<br />recovery period after presservation. The study was conducted<br />at Tissue Culture Laboratory in Indonesian Center for<br />Agricultural Biotechnology and Genetic Resources Research<br />and Development from August 2006 to July 2007. The<br />activities included propagation of in vitro shoot grown in<br />vitro as explants source, preservation of in vitro shoots of<br />pruatjan, and regeneration of the cultures after preservation.<br />The experiment was designed as factorial in Randomized<br />Completely Block Design with 6 replications. The DKW basal<br />media containing 1 ppm BA, 0.2 ppm thidiazuron, and 100<br />ppm arginine were supplemented with mannitol or sucrose<br />at the level of 1, 2, 3, 4, and 5%. The observed variables were<br />total number of leaves, number of shoot, and number of wilt<br />leaves. The result revealed that pruatjan cultures could be<br />stored longer than 4 months. Generally, the effect of<br />mannitol or sucrose was more dominant than that of cultures<br />condition. The mannitol (1-5%) strongly inhibited the<br />growth of pruatjan cultures so that only a few cultures<br />survived at 7 months preservation period and needed about<br />1 month for recovery. On the contrary, the effect of sucrose<br />(at the same level) was better than mannitol. The 2.5%<br />sucrose optimally inhibited pruatjan cultures. At that condition,<br />the cultures could be stored for 10 months without<br />morphological changes so that they could recover spontaneously.</p>

Perbanyakan Tanaman Chrysanthemum pada Kondisi Fotoautotropik Secara in Vitro

“…Sukrosa yang ditambahkan ke dalam media kultur yang memiliki konsentrasi tinggi, dapat menurunkan potensial osmotik sehingga nutrisi yang menyebar ke dalam jaringan eksplan atau tanaman akan berjalan lambat. Aliran nutrisi lambat inilah yang menyebabkan pertumbuhan kultur atau munculnya tunas juga terhambat (Roostika et al 2017).…”

Development of Photomyxotrophic Culture Protocol of Lemongrass Through Sucrose Concentration Increase and Light Intensity Decrease

“…However, the addition of high concentrations Desy Irmawati & Enni Suwarsi Rahayu / Biosaintifika 15 (2) (2023): 212-219 of sucrose can cause inhibition of culture growth due to a decrease in the osmotic potential of the medium which causes the nutrient flow to run slowly (Roostika et al, 2017). The results of this study are in line with the research of Triyastuti et al (2018) that the sucrose concentration of 20 g/l was able to produce the highest shoots, the widest leaves, and to encourage the emergence of faster shoots in chrysanthemum plantlets.…”

Pembentukan Benih Sintetik Tanaman Nenas

“…Jika paklobutrazol menghambat pertumbuhan melalui pemblokiran biosintesis giberelin (Arteca 1996), manitol merupakan gula alkohol yang berdifusi di bagian ekstraseluler namun tidak dapat memasuki sel (Taiz & Zeiger 2003), sehingga berfungsi sebagai regulator osmotik. Penggunaan regulator osmotik terbukti dapat menyimpan biakan ubi jalar selama 10 bulan (Roostika et al 2001), biakan kentang hitam selama 3 bulan (Roostika et al 2005), biakan purwoceng selama 7 bulan (Roostika et al 2008), dan biakan jeruk besar selama 5 bulan (Dewi et al 2010).…”