The Importance of Rhamnolipid-Biosurfactant-Induced Changes in Bacterial Membrane Lipids of Bacillus subtilis for the Antimicrobial Activity of Thiosulfonates

Characterization and antifungal properties of rhamnolipids produced by mangrove sediment bacterium Pseudomonas aeruginosa strain KVD-HM52

“…It has been reported the mechanisms of the action of rhamnolipids on inhibition of pathogenic bacteria and fungi are mainly through damaging microbial cell membranes, reducing spore movement and leading to spore collapse (Sotirova et al, 2012). Earlier studies also reported that many strains from Pseudomonas sp.…”

Biocontrol of post‐harvest Alternaria alternata decay of cherry tomatoes with rhamnolipids and possible mechanisms of action

“…After incubation period, MIC, defined as the lowest concentration of laurel oil that inhibits development of visible microbial growth on PDB was determined. To determine the MFC, subcultures were grown on inhibitor-free nutrient agar plates from each clear tube in the MIC test series, followed by incubation at 27 ∘ C for 48 h. 20 In vivo assays Cherry tomatoes were treated as described above. Each wound was treated with 30 μL of (1) sterile distilled water as the control;…”

Use of rhamnolipid biosurfactant for membrane biofouling prevention and cleaning

“…In the current study rhamnolipids did not affect bacterial growth (Figure 1a and b), consistent with a previous study in which the rhamnolipid-PS, purified from Pseudomonas sp. PS-17, had no effect on growth of P. aeruginosa NBIMCC1390 at 10 mg l −1 (Sotirova et al 2012;Sodagari et al 2013). In the current study the percentage of dead cells increased, although no effect on bacterial growth was observed.…”

“…To measure the total cell number, the samples were stained with 0.1 mg ml −1 of 4′,6-diamidino-2-phenylindole (DAPI), a fluorescent dye used for staining double strand DNA, and viability was assessed using LIVE/DEAD BacLight bacterial viability kits (Molecular Probes, Eugene, OR, USA). The Live/ Dead ® viability kit utilized contained Syto ® 9 and propidium iodide (PI); bacteria with intact cell membranes fluoresce green, whereas bacteria with damaged membranes fluoresce red (Sotirova et al 2012). After staining, fluorescent images were captured using a LSM5 inverted confocal laser scanning microscope (CLSM; Zeiss, Oberkochen, Germany).…”