Biocontrol of post‐harvest Alternaria alternata decay of cherry tomatoes with rhamnolipids and possible mechanisms of action

Abstract

Rhamnolipids could be an alternative to chemicals for controlling post-harvest phytopathogenic fungi on fruits and vegetables.

Effects of cold plasma, UV‐C or aqueous ozone treatment on Botrytis cinerea and their potential application in preserving blueberry

“…The supernates were used for nucleic acid leakage determination according to Yan et al . (). The determined absorbance was 260 nm, and the unit was expressed with the absorbance.…”

“…Fungal hyphae were washed twice using distilled water and used for wet weight determination. The supernates were used for nucleic acid leakage determination according to Yan et al (2015). The determined absorbance was 260 nm, and the unit was expressed with the absorbance.…”

Efficacy of a rhamnolipid biosurfactant to inhibit Trichophyton rubrum in vitro and in a mice model of dermatophytosis

“…The effect of RL on mycelia of T. rubrum was observed using scanning electron microscopy (SEM), atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM). SEM was performed according to Yan et al with some modifications. Mycelia of T. rubrum were excised from an active culture grown in SDA plates and transferred aseptically to SDB prepared in RL solution (500 μg/mL) and incubated at 25°C for 7 days on a rotary shaker (200 rpm).…”

“…Absorbance at 260 nm was recorded to estimate the amount of nucleic acids released from the cytoplasm of the RL‐treated fungal mycelia. The experiment was performed according to Yan et al A 14‐day culture of T. rubrum in SDB with or without RL at concentrations of 250, 500 and 1000 μg/mL incubated at 25°C was centrifuged, and the supernatants were collected. The absorbance of the leaked nucleic acid in the supernatant was measured at 260 nm with a nucleic acid analyser (Nano Drop 2000C, Thermo Scientific).…”

Glycolipid biosurfactants: main properties and potential applications in agriculture and food industry

“…pisi in Pisum sativum. 49 Moreover, Yan et al 50,51 demonstrated the efficiency of rhamnolipids for treating the decay incidences of A. alternata on cherry tomatoes (Lycopersicon esculentum). Similarly, Sharma et al 52 demonstrated the involvement of Pseudomonas sp.…”