The importance of PU.1 concentration in hematopoietic lineage commitment and maturation

Ski can negatively regulates macrophage differentiation through its interaction with PU.1

“…Most importantly, graded changes in each factor's concentration could result in committing a hematopoietic progenitor cell fate (Dahl and Simon, 2003;Rosenbauer et al, 2004). Our findings have supported general important roles of Ski in the hematopoietic cell system by inhibiting the function of critical transcription factors including PU.1 and GATA1.…”

“…Since GATA1 and PU.1, a myeloid-and B-cell-specific E26 transformation-specific (ETS) transcription factor (Dahl and Simon, 2003), are known to antagonize each other's activity for specification of the hematopoietic cell system (Rekhtman et al, 1999;Zhang et al, 2000), we investigated the possibility of a functional link between Ski and PU.1. In this report, we show that Ski blocks PU.1-induced transcriptional activation by mediating interaction between PU.1 and HDAC3.…”

A cascade of Ca2+/calmodulin-dependent protein kinases regulates the differentiation and functional activation of murine neutrophils

“…Identifying the precise mechanism by which CKLiK interferes with neutrophil gene expression will require more detailed analyses, but CKLiK may target a repressor of neutrophil differentiation, such as the CCAAT displacement protein (CDP/cut) or the transcription factor PU.1. Both factors can repress neutrophil gene expression, and their activities have been suggested to be modulated by serine phosphorylation [24,[37][38][39][40][41]. Interestingly, CDP represses the expression of C/EBPε, lactoferrin and gp91 phox , all three of which were inhibited in cells expressing the constitutively active CKLiK.…”

Identification of IRF-8 and IRF-1 target genes in activated macrophages

“…Unlike other IRF members, IRF-8 is capable of binding to its target DNA sequence only following association with either IRF-1 and IRF-2 or non-IRF transcription factors, such as PU.1, an essential factor for hematopoiesis (Sharf et al 1997;Wang et al 2000;Dahl and Simon 2003) The domain essential for these protein-protein associations, termed IRF Association Domain (IAD), is conserved among all other IRF members excluding IRF-1 and IRF-2. The association modules of IRF-1 and IRF-2 with IRF-8 were identified as PEST domains, enriched with proline, glutamic acid, serine, and threonine, originally shown in PU.1 .The specific partner that interacts with IRF-8 dictates not only the DNA binding site but also the transcriptional activity e.g.…”