The Importance of Polarized Light Microscopy in the Analytical Setting

Sparenga, Sebastian B.

doi:10.1017/s1431927608083694

Cited by 4 publications

(3 citation statements)

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“…4 that two seams were formed at the boundary to the original plate morphology due to the outward melt flow, which is consistent with previous reports [20,21]. Two positions as denoted were chosen in each weld to study the orientation of the macromolecules.…”

Section: Resultssupporting

confidence: 84%

Measuring the orientation of chains in polypropylene welds by infrared microscopy: A tool to understand the impact of thermo-mechanical treatment and processing

Damodaran

Schuster

Rode

et al. 2015

Polymer

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Section: Resultssupporting

confidence: 84%

Measuring the orientation of chains in polypropylene welds by infrared microscopy: A tool to understand the impact of thermo-mechanical treatment and processing

Damodaran

Schuster

Rode

et al. 2015

Polymer

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“…Indeed, the ability to combine fluorescence, transmittance, and polarization microscopy measurements could be useful in obtaining new insights into the organization of molecules in biological specimens [3]. Similar to conventional, transmitted light microscopy, polarization microscopy instruments can employ polarized, transmitted light to study the structure of supramolecular aggregates within individual cells [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Detecting ordered small molecule drug aggregates in live macrophages: a multi-parameter microscope image data acquisition and analysis strategy

Rzeczycki

Yoon

Keswani

et al. 2017

Biomed. Opt. Express

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Abstract:Following prolonged administration, certain orally bioavailable but poorly soluble small molecule drugs are prone to precipitate out and form crystal-like drug inclusions (CLDIs) within the cells of living organisms. In this research, we present a quantitative multiparameter imaging platform for measuring the fluorescence and polarization diattenuation signals of cells harboring intracellular CLDIs. To validate the imaging system, the FDAapproved drug clofazimine (CFZ) was used as a model compound. Our results demonstrated that a quantitative multi-parameter microscopy image analysis platform can be used to study drug sequestering macrophages, and to detect the formation of ordered molecular aggregates formed by poorly soluble small molecule drugs in animals.

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“…The polarized light microscope has demonstrated its value as an indispensable analytical instrument with continuous usage and the development of new applications for more than 170 years (Weaver, 2003). It now plays an important role in physics (Kaminsky et al, 2010), petrography (Higgins, 2010), microchemistry (Sparenga, 2008), and biology (van Turnhout et al, 2009). The polarized light microscope makes use of the characteristics of anisotropic hyalosome that it will form a polarized light beam when nature light permeates.…”

Section: Introductionmentioning

confidence: 99%

Identification of powdered Chinese herbal medicines by fluorescence microscopy, Part 1: Fluorescent characteristics of mechanical tissues, conducting tissues, and ergastic substances

Wang

Liang

et al. 2011

Microscopy Res & Technique

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The light microscope has been successfully used in identification of Chinese herbal medicines (CHMs) for more than a century. However, positive identification is not always possible. Given the popularity of fluorescence microscopy in bioanalysis, researchers dedicated to finding new ways to identify CHMs more effectively are now turning to fluorescence microscopy for authentication purposes. Some studies on distinguishing confused species from the same genus and on exploring distributions of chemicals in tissues of CHMs by fluorescence microscopy have been reported; however, no systematic investigations on fluorescent characteristics of powdered CHMs have been reported. Here, 46 samples of 16 CHMs were investigated. Specifically, the mechanical tissues including stone cells and fibers, the conducting tissues including three types of vessels, and ergastic substances including crystals of calcium oxalate and secretions, in various powdered CHMs were investigated by both light microscope and fluorescence microscope. The results showed many microscopic features emit fluorescence that makes them easily observed, even against complex backgrounds. Under the fluorescence microscope, different microscopic features from the same powdered CHM or some same features from different powdered CHMs emitted the different fluorescence, making this information very helpful for the authentication of CHMs in powder form. Moreover, secretions with unique chemical profiles from different powdered CHMs showed different fluorescent characteristics. Hence, fluorescence microscopy could be a useful additional method for the authentication of powdered CHMs if the fluorescent characteristics of specific CHMs are known.

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The Importance of Polarized Light Microscopy in the Analytical Setting

Abstract: Extended abstract of a paper presented at Microscopy and Microanalysis 2008 in Albuquerque, New Mexico, USA, August 3 – August 7, 2008

Cited by 4 publications

References 0 publications

Measuring the orientation of chains in polypropylene welds by infrared microscopy: A tool to understand the impact of thermo-mechanical treatment and processing

Measuring the orientation of chains in polypropylene welds by infrared microscopy: A tool to understand the impact of thermo-mechanical treatment and processing

Detecting ordered small molecule drug aggregates in live macrophages: a multi-parameter microscope image data acquisition and analysis strategy

Identification of powdered Chinese herbal medicines by fluorescence microscopy, Part 1: Fluorescent characteristics of mechanical tissues, conducting tissues, and ergastic substances

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